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Effect of divalent cations on pore formation and degradation of poly(D,L-lactide-co-glycolide).

Fredenberg, Susanne LU ; Reslow, Mats and Axelsson, Anders LU (2007) In Pharmaceutical Development and Technology 12(6). p.563-572
Abstract
Poly(D,L-lactide-co-glycolide) (PLG) is probably the biodegradable polymer most often used for polymeric controlled-release formulations. Different salts have been shown to affect the swelling and degradation of PLG, which, in turn, affect the release of encapsulated drugs. In this investigation the effect of divalent cations was especially investigated. Films of PLG were incubated in phosphate buffer saline (PBS), a buffer containing salts similar to plasma, Hepes buffer, and Hepes buffer with ZnCl2, CaCl2, MgCl2, or Na2CO3 added. Pore formation at the surface and inside the film was analyzed by scanning electron microscopy. The samples were also analyzed gravimetrically at predetermined intervals to determine the mass loss, and for some... (More)
Poly(D,L-lactide-co-glycolide) (PLG) is probably the biodegradable polymer most often used for polymeric controlled-release formulations. Different salts have been shown to affect the swelling and degradation of PLG, which, in turn, affect the release of encapsulated drugs. In this investigation the effect of divalent cations was especially investigated. Films of PLG were incubated in phosphate buffer saline (PBS), a buffer containing salts similar to plasma, Hepes buffer, and Hepes buffer with ZnCl2, CaCl2, MgCl2, or Na2CO3 added. Pore formation at the surface and inside the film was analyzed by scanning electron microscopy. The samples were also analyzed gravimetrically at predetermined intervals to determine the mass loss, and for some samples the pH within the PLG films was determined by confocal microscopy. Pores were formed faster in the presence of all divalent cations, and the results indicated a greater degradation rate in the presence of Zn2+. The catalyzing effect of the divalent cations on degradation was attributed to their ability to act as Lewis acids. Pores were formed more slowly in PBS than in a buffer containing salts similar to plasma, which should be considered when choosing the in vitro release medium. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Lactic Acid: chemistry, Polyglycolic Acid: chemistry, Polymers: chemistry
in
Pharmaceutical Development and Technology
volume
12
issue
6
pages
563 - 572
publisher
Taylor & Francis
external identifiers
  • pmid:18161629
  • wos:000252090500003
  • scopus:37549021155
ISSN
1083-7450
DOI
10.1080/10837450701560588
language
English
LU publication?
yes
id
b356c952-4255-4650-9878-7df56f70ff73 (old id 1034912)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18161629?dopt=Abstract
date added to LUP
2009-07-08 17:02:11
date last changed
2017-07-30 04:26:56
@article{b356c952-4255-4650-9878-7df56f70ff73,
  abstract     = {Poly(D,L-lactide-co-glycolide) (PLG) is probably the biodegradable polymer most often used for polymeric controlled-release formulations. Different salts have been shown to affect the swelling and degradation of PLG, which, in turn, affect the release of encapsulated drugs. In this investigation the effect of divalent cations was especially investigated. Films of PLG were incubated in phosphate buffer saline (PBS), a buffer containing salts similar to plasma, Hepes buffer, and Hepes buffer with ZnCl2, CaCl2, MgCl2, or Na2CO3 added. Pore formation at the surface and inside the film was analyzed by scanning electron microscopy. The samples were also analyzed gravimetrically at predetermined intervals to determine the mass loss, and for some samples the pH within the PLG films was determined by confocal microscopy. Pores were formed faster in the presence of all divalent cations, and the results indicated a greater degradation rate in the presence of Zn2+. The catalyzing effect of the divalent cations on degradation was attributed to their ability to act as Lewis acids. Pores were formed more slowly in PBS than in a buffer containing salts similar to plasma, which should be considered when choosing the in vitro release medium.},
  author       = {Fredenberg, Susanne and Reslow, Mats and Axelsson, Anders},
  issn         = {1083-7450},
  keyword      = {Lactic Acid: chemistry,Polyglycolic Acid: chemistry,Polymers: chemistry},
  language     = {eng},
  number       = {6},
  pages        = {563--572},
  publisher    = {Taylor & Francis},
  series       = {Pharmaceutical Development and Technology},
  title        = {Effect of divalent cations on pore formation and degradation of poly(D,L-lactide-co-glycolide).},
  url          = {http://dx.doi.org/10.1080/10837450701560588},
  volume       = {12},
  year         = {2007},
}