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Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.

Quillaguaman, Jorge LU ; Doan Van, Thuoc LU ; Guzmán, Hector LU ; Guzmán, Daniel LU ; Martín, Javier; Everest, Akaraonye and Hatti-Kaul, Rajni LU (2008) In Applied Microbiology and Biotechnology 78(2). p.227-232
Abstract
High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch... (More)
High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Microbiology and Biotechnology
volume
78
issue
2
pages
227 - 232
publisher
Springer
external identifiers
  • pmid:18097660
  • wos:000252614200005
  • scopus:38649122072
ISSN
1432-0614
DOI
10.1007/s00253-007-1297-x
language
English
LU publication?
yes
id
2fd5ad70-6ce2-4051-8ae6-3e5a8e331448 (old id 1035009)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18097660?dopt=Abstract
date added to LUP
2009-07-10 14:20:57
date last changed
2017-11-05 04:13:18
@article{2fd5ad70-6ce2-4051-8ae6-3e5a8e331448,
  abstract     = {High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1).},
  author       = {Quillaguaman, Jorge and Doan Van, Thuoc and Guzmán, Hector and Guzmán, Daniel and Martín, Javier and Everest, Akaraonye and Hatti-Kaul, Rajni},
  issn         = {1432-0614},
  language     = {eng},
  number       = {2},
  pages        = {227--232},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.},
  url          = {http://dx.doi.org/10.1007/s00253-007-1297-x},
  volume       = {78},
  year         = {2008},
}