Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.
(2008) In Applied Microbiology and Biotechnology 78(2). p.227-232- Abstract
- High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch... (More)
- High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1035009
- author
- Quillaguaman, Jorge LU ; Doan Van, Thuoc LU ; Guzmán, Hector LU ; Guzmán, Daniel LU ; Martín, Javier ; Everest, Akaraonye and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Microbiology and Biotechnology
- volume
- 78
- issue
- 2
- pages
- 227 - 232
- publisher
- Springer
- external identifiers
-
- pmid:18097660
- wos:000252614200005
- scopus:38649122072
- pmid:18097660
- ISSN
- 1432-0614
- DOI
- 10.1007/s00253-007-1297-x
- language
- English
- LU publication?
- yes
- id
- 2fd5ad70-6ce2-4051-8ae6-3e5a8e331448 (old id 1035009)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18097660?dopt=Abstract
- date added to LUP
- 2016-04-01 14:43:32
- date last changed
- 2022-03-06 20:52:05
@article{2fd5ad70-6ce2-4051-8ae6-3e5a8e331448,
abstract = {{High poly(3-hydroxybutyrate) (PHB) content and volumetric productivity were achieved by fed-batch culture of Halomonas boliviensis using a defined medium. Initial shake flask cultivations in a minimal medium revealed that the growth of H. boliviensis was supported only when the medium was supplemented with aspartic acid, glycine, or glutamine. Addition of 0.1% (w/v) glutamine in the medium resulted in the highest cell dry weight (CDW; 3.9 g l(-1)). Glutamine was replaced by the less expensive monosodium glutamate (MSG) in the medium without any notable change in the final cell density. Effect of initial concentrations of NH(4)Cl and K(2)HPO(4) on cell growth and PHB accumulation by H. boliviensis was then analyzed using a fed-batch fermentation system. The best conditions for PHB production by H. boliviensis were attained using 0.4% (w/v) NH(4)Cl and 0.22% (w/v) K(2)HPO(4) and adding MSG intermittently to the fermentor. Poly(3-hydroxybutyrate) content and CDW reached 90 wt.% and 23 g l(-1), respectively, after 18 h of cultivation. In order to increase CDW and PHB content, MSG, NH(4)Cl, and K(2)HPO(4) were initially fed to the fermentor to maintain their concentrations at 2%, 0.4%, and 0.22% (w/v), respectively, and subsequently their feed was suppressed. This resulted in a CDW of 44 g l(-1), PHB content of 81 wt.%, and PHB volumetric productivity of 1.1 g l(-1) h(-1).}},
author = {{Quillaguaman, Jorge and Doan Van, Thuoc and Guzmán, Hector and Guzmán, Daniel and Martín, Javier and Everest, Akaraonye and Hatti-Kaul, Rajni}},
issn = {{1432-0614}},
language = {{eng}},
number = {{2}},
pages = {{227--232}},
publisher = {{Springer}},
series = {{Applied Microbiology and Biotechnology}},
title = {{Poly(3-hydroxybutyrate) production by Halomonas boliviensis in fed-batch culture.}},
url = {{http://dx.doi.org/10.1007/s00253-007-1297-x}},
doi = {{10.1007/s00253-007-1297-x}},
volume = {{78}},
year = {{2008}},
}