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Nonimmunodominant regions are effective as building blocks in a streptococcal fusion protein vaccine.

Stålhammar-Carlemalm, Margaretha LU ; Waldemarsson, Johan LU ; Johnsson, Eskil LU ; Areschoug, Thomas LU and Lindahl, Gunnar LU (2007) In Cell Host and Microbe 2(6). p.427-434
Abstract
Identification of antigens that elicit protective immunity is essential for effective vaccine development. We investigated the related surface proteins of group B Streptococcus, Rib and alpha, as potential vaccine candidates. Paradoxically, nonimmunodominant regions proved to be of particular interest as vaccine components. Mouse antibodies elicited by Rib and alpha were directed almost exclusively against the C-terminal repeats and not against the N-terminal regions. However, a fusion protein derived from the nonimmunodominant N-terminal regions of Rib and alpha was much more immunogenic than one derived from the repeats and was immunogenic even without adjuvant. Moreover, antibodies to the N-terminal fusion protein protected against... (More)
Identification of antigens that elicit protective immunity is essential for effective vaccine development. We investigated the related surface proteins of group B Streptococcus, Rib and alpha, as potential vaccine candidates. Paradoxically, nonimmunodominant regions proved to be of particular interest as vaccine components. Mouse antibodies elicited by Rib and alpha were directed almost exclusively against the C-terminal repeats and not against the N-terminal regions. However, a fusion protein derived from the nonimmunodominant N-terminal regions of Rib and alpha was much more immunogenic than one derived from the repeats and was immunogenic even without adjuvant. Moreover, antibodies to the N-terminal fusion protein protected against infection and inhibited bacterial invasion of epithelial cells. Similarly, the N-terminal region of Streptococcus pyogenes M22 protein, which is targeted by opsonic antibodies, is nonimmunodominant. These data indicate that nonimmunodominant regions of bacterial antigens could be valuable for vaccine development. (Less)
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published
subject
keywords
Epithelial Cells: microbiology, Epitopes: immunology, ISCOMs: immunology, Membrane Proteins: immunology, Streptococcal Infections: blood, Streptococcal Infections: prevention & control, Streptococcal Vaccines: immunology, Streptococcus pyogenes: immunology, Streptococcus agalactiae: immunology, Bacterial Proteins: immunology, Antigens, Surface: immunology, Bacterial: immunology, Antibodies, Bacterial: blood
in
Cell Host and Microbe
volume
2
issue
6
pages
427 - 434
publisher
Elsevier
external identifiers
  • pmid:18078694
  • wos:000251855600008
  • scopus:36749079247
ISSN
1934-6069
DOI
10.1016/j.chom.2007.10.003
language
English
LU publication?
yes
id
0988d4a0-6b61-4126-b847-5b8f0f7de5b2 (old id 1035302)
date added to LUP
2008-08-20 13:56:07
date last changed
2017-08-20 03:37:15
@article{0988d4a0-6b61-4126-b847-5b8f0f7de5b2,
  abstract     = {Identification of antigens that elicit protective immunity is essential for effective vaccine development. We investigated the related surface proteins of group B Streptococcus, Rib and alpha, as potential vaccine candidates. Paradoxically, nonimmunodominant regions proved to be of particular interest as vaccine components. Mouse antibodies elicited by Rib and alpha were directed almost exclusively against the C-terminal repeats and not against the N-terminal regions. However, a fusion protein derived from the nonimmunodominant N-terminal regions of Rib and alpha was much more immunogenic than one derived from the repeats and was immunogenic even without adjuvant. Moreover, antibodies to the N-terminal fusion protein protected against infection and inhibited bacterial invasion of epithelial cells. Similarly, the N-terminal region of Streptococcus pyogenes M22 protein, which is targeted by opsonic antibodies, is nonimmunodominant. These data indicate that nonimmunodominant regions of bacterial antigens could be valuable for vaccine development.},
  author       = {Stålhammar-Carlemalm, Margaretha and Waldemarsson, Johan and Johnsson, Eskil and Areschoug, Thomas and Lindahl, Gunnar},
  issn         = {1934-6069},
  keyword      = {Epithelial Cells: microbiology,Epitopes: immunology,ISCOMs: immunology,Membrane Proteins: immunology,Streptococcal Infections: blood,Streptococcal Infections: prevention & control,Streptococcal Vaccines: immunology,Streptococcus pyogenes: immunology,Streptococcus agalactiae: immunology,Bacterial Proteins: immunology,Antigens,Surface: immunology,Bacterial: immunology,Antibodies,Bacterial: blood},
  language     = {eng},
  number       = {6},
  pages        = {427--434},
  publisher    = {Elsevier},
  series       = {Cell Host and Microbe},
  title        = {Nonimmunodominant regions are effective as building blocks in a streptococcal fusion protein vaccine.},
  url          = {http://dx.doi.org/10.1016/j.chom.2007.10.003},
  volume       = {2},
  year         = {2007},
}