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Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization.

Hedström, Martin LU ; Plieva, Fatima LU ; Galaev, Igor LU and Mattiasson, Bo LU (2008) In Analytical and Bioanalytical Chemistry 390(3). p.907-912
Abstract
A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the... (More)
A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the matrix allowed for the development of a generic, high-performance online protein digestion system utilizing the wall-bound proteases. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chitosan, Macroporous, Cryogel, Scaffold, Staphylococcal enterotoxin B
in
Analytical and Bioanalytical Chemistry
volume
390
issue
3
pages
907 - 912
publisher
Springer
external identifiers
  • pmid:18066683
  • wos:000252417800015
  • scopus:38348999498
ISSN
1618-2642
DOI
10.1007/s00216-007-1745-6
language
English
LU publication?
yes
id
adda712b-23e1-4663-b094-8f37581d1a28 (old id 1035491)
date added to LUP
2008-05-23 09:35:33
date last changed
2017-05-14 03:34:15
@article{adda712b-23e1-4663-b094-8f37581d1a28,
  abstract     = {A novel monolithic macroporous material was developed by cross-linking hen egg albumin (HEA) and chitosan with glutaraldehyde at subzero temperatures. A macroporous cryogel structure allowed efficient mass transport of solutes within the material. In one application, albumin was partially replaced with active enzymes (glucose oxidase and horseradish peroxidase) resulting in the production of macroporous biocatalyst preparations suitable for flow-injection analysis of glucose in the low millimolar range. In another application, the proteolytic enzymes savinase and esperase were coupled to the macroporous structure via free amino groups on the pore walls using glutaraldehyde as cross-linker/spacer agent. The low hydraulic resistance of the matrix allowed for the development of a generic, high-performance online protein digestion system utilizing the wall-bound proteases.},
  author       = {Hedström, Martin and Plieva, Fatima and Galaev, Igor and Mattiasson, Bo},
  issn         = {1618-2642},
  keyword      = {Chitosan,Macroporous,Cryogel,Scaffold,Staphylococcal enterotoxin B},
  language     = {eng},
  number       = {3},
  pages        = {907--912},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization.},
  url          = {http://dx.doi.org/10.1007/s00216-007-1745-6},
  volume       = {390},
  year         = {2008},
}