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Mixed carbon sources for nitrate reduction in activated sludge-identification of bacteria and process activity studies.

Hagman, Marinette LU ; Nielsen, J L; Nielsen, P H and la Cour Jansen, Jes LU (2008) In Water Research 42(6-7). p.1539-1546
Abstract
Mixtures of methanol and acetate as carbon source were investigated in order to determine their capacity to enhance denitrification and for analysis of the microbial composition and carbon degradation activity in activated sludge from wastewater treatment plants. Laboratory batch reactors at 20 degrees C were used for nitrate uptake rate (NUR) measurements in order to investigate the anoxic activity, while single and mixed carbon substrates were added to activated sludge. Microautoradiography (MAR) in combination with fluorescence in situ hybridisation (FISH) were applied for microbial analysis during exposure to different carbon sources. The NUR increased with additions of a mixture of acetate and methanol compared with additions of a... (More)
Mixtures of methanol and acetate as carbon source were investigated in order to determine their capacity to enhance denitrification and for analysis of the microbial composition and carbon degradation activity in activated sludge from wastewater treatment plants. Laboratory batch reactors at 20 degrees C were used for nitrate uptake rate (NUR) measurements in order to investigate the anoxic activity, while single and mixed carbon substrates were added to activated sludge. Microautoradiography (MAR) in combination with fluorescence in situ hybridisation (FISH) were applied for microbial analysis during exposure to different carbon sources. The NUR increased with additions of a mixture of acetate and methanol compared with additions of a single carbon source. MAR-FISH measurements demonstrated that the probe-defined group of Azoarcus was the main group of bacteria utilising acetate and the only active group utilising methanol under anoxic conditions. The present study indicated an improved denitrification potential by additions of a mixed carbon source compared with commonly used single-carbon additions. It is also established that Azoarcus bacteria are involved in the degradation of both acetate and methanol in the anoxic activated sludge. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Water Research
volume
42
issue
6-7
pages
1539 - 1546
publisher
Elsevier
external identifiers
  • pmid:18061233
  • wos:000255166600020
  • scopus:40749141614
ISSN
1879-2448
DOI
10.1016/j.watres.2007.10.034
language
English
LU publication?
yes
id
399dfa6f-cd6d-4dee-be2a-7e5dab3f291d (old id 1035598)
date added to LUP
2008-04-03 14:35:25
date last changed
2017-09-24 04:01:34
@article{399dfa6f-cd6d-4dee-be2a-7e5dab3f291d,
  abstract     = {Mixtures of methanol and acetate as carbon source were investigated in order to determine their capacity to enhance denitrification and for analysis of the microbial composition and carbon degradation activity in activated sludge from wastewater treatment plants. Laboratory batch reactors at 20 degrees C were used for nitrate uptake rate (NUR) measurements in order to investigate the anoxic activity, while single and mixed carbon substrates were added to activated sludge. Microautoradiography (MAR) in combination with fluorescence in situ hybridisation (FISH) were applied for microbial analysis during exposure to different carbon sources. The NUR increased with additions of a mixture of acetate and methanol compared with additions of a single carbon source. MAR-FISH measurements demonstrated that the probe-defined group of Azoarcus was the main group of bacteria utilising acetate and the only active group utilising methanol under anoxic conditions. The present study indicated an improved denitrification potential by additions of a mixed carbon source compared with commonly used single-carbon additions. It is also established that Azoarcus bacteria are involved in the degradation of both acetate and methanol in the anoxic activated sludge.},
  author       = {Hagman, Marinette and Nielsen, J L and Nielsen, P H and la Cour Jansen, Jes},
  issn         = {1879-2448},
  language     = {eng},
  number       = {6-7},
  pages        = {1539--1546},
  publisher    = {Elsevier},
  series       = {Water Research},
  title        = {Mixed carbon sources for nitrate reduction in activated sludge-identification of bacteria and process activity studies.},
  url          = {http://dx.doi.org/10.1016/j.watres.2007.10.034},
  volume       = {42},
  year         = {2008},
}