Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.
(2016) In Methods in Molecular Biology 1396. p.51-65- Abstract
- Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8504444
- author
- Rödström, Karin LU and Lindkvist, Karin LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 1396
- pages
- 51 - 65
- publisher
- Springer
- external identifiers
-
- pmid:26676036
- scopus:84950279250
- pmid:26676036
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-4939-3344-0_4
- language
- English
- LU publication?
- yes
- id
- 1037d161-ce36-41ce-b905-06396a6fe4c3 (old id 8504444)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26676036?dopt=Abstract
- date added to LUP
- 2016-04-04 08:39:25
- date last changed
- 2022-01-29 03:48:18
@article{1037d161-ce36-41ce-b905-06396a6fe4c3,
abstract = {{Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.}},
author = {{Rödström, Karin and Lindkvist, Karin}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{51--65}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.}},
url = {{http://dx.doi.org/10.1007/978-1-4939-3344-0_4}},
doi = {{10.1007/978-1-4939-3344-0_4}},
volume = {{1396}},
year = {{2016}},
}