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Light-induced immobilization of biomolecules as an attractive alternative to micro-droplet dispensing-based arraying technologies

Duroux, Meg; Skovsen, Esben; Neves-Petersen, Maria Teresa; Duroux, Laurent; Gurevich, Lenoid; Borrebaeck, Carl LU ; Wingren, Christer LU and Petersen, Steffen (2008) In Proteomics 7(19). p.1113-1113
Abstract
The present work shows how UV light-induced molecular immobilisation (LIMI) of biomolecules onto thiol reactive surfaces can be used to make biosensors, without the need for traditional microdispensing technologies. Using LIMI, arrays of biomolecules can be created with a high degree of reproducibility. This technology can be used to circumvent the need for often expensive nano/microdispensing technologies. The ultimate size of the immobilised spots is defined by the focal area of the UV beam, which for a diffraction-limited beam can be less than 1 m in diameter. LIMI has the added benefit that the immobilised molecules will be spatially oriented and covalently bound to the surface. The activity of the sensor molecules is retained.... (More)
The present work shows how UV light-induced molecular immobilisation (LIMI) of biomolecules onto thiol reactive surfaces can be used to make biosensors, without the need for traditional microdispensing technologies. Using LIMI, arrays of biomolecules can be created with a high degree of reproducibility. This technology can be used to circumvent the need for often expensive nano/microdispensing technologies. The ultimate size of the immobilised spots is defined by the focal area of the UV beam, which for a diffraction-limited beam can be less than 1 m in diameter. LIMI has the added benefit that the immobilised molecules will be spatially oriented and covalently bound to the surface. The activity of the sensor molecules is retained. Antibody sensor arrays made using LIMI demonstrated successful antigen binding. In addition, the pattern of immobilised molecules on the surface is not restricted to conventional array formats. The ultimate consequence of the LIMI is that it is possible to write complex protein patterns using bitmaps at high resolution onto substrates. Thus, LIMI of biomolecules provides a new technological platform for biomolecular immobilisation and the potential for replacing present microdispensing arraying technologies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Proteomics
volume
7
issue
19
pages
1113 - 1113
publisher
John Wiley & Sons
external identifiers
  • wos:000254262500017
  • scopus:35348976064
ISSN
1615-9861
DOI
10.1002/pmic.200700472
language
English
LU publication?
yes
id
52e2350e-d4fe-42bf-a94d-27a8e070366a (old id 1040244)
date added to LUP
2008-02-28 14:08:59
date last changed
2017-06-25 04:33:39
@article{52e2350e-d4fe-42bf-a94d-27a8e070366a,
  abstract     = {The present work shows how UV light-induced molecular immobilisation (LIMI) of biomolecules onto thiol reactive surfaces can be used to make biosensors, without the need for traditional microdispensing technologies. Using LIMI, arrays of biomolecules can be created with a high degree of reproducibility. This technology can be used to circumvent the need for often expensive nano/microdispensing technologies. The ultimate size of the immobilised spots is defined by the focal area of the UV beam, which for a diffraction-limited beam can be less than 1 m in diameter. LIMI has the added benefit that the immobilised molecules will be spatially oriented and covalently bound to the surface. The activity of the sensor molecules is retained. Antibody sensor arrays made using LIMI demonstrated successful antigen binding. In addition, the pattern of immobilised molecules on the surface is not restricted to conventional array formats. The ultimate consequence of the LIMI is that it is possible to write complex protein patterns using bitmaps at high resolution onto substrates. Thus, LIMI of biomolecules provides a new technological platform for biomolecular immobilisation and the potential for replacing present microdispensing arraying technologies.},
  author       = {Duroux, Meg and Skovsen, Esben and Neves-Petersen, Maria Teresa and Duroux, Laurent and Gurevich, Lenoid and Borrebaeck, Carl and Wingren, Christer and Petersen, Steffen},
  issn         = {1615-9861},
  language     = {eng},
  number       = {19},
  pages        = {1113--1113},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics},
  title        = {Light-induced immobilization of biomolecules as an attractive alternative to micro-droplet dispensing-based arraying technologies},
  url          = {http://dx.doi.org/10.1002/pmic.200700472},
  volume       = {7},
  year         = {2008},
}