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Early development of retinal subtypes in long-term cultures of human embryonic retina.

Engelsberg, Karl LU ; Ehinger, Berndt LU and Ghosh, Fredrik LU (2008) In Current Eye Research 33(2). p.185-191
Abstract
Purpose: To study early signs of neuronal and glial differentiation in the human embryonic retina. Materials and Methods: 6.5-to 8-week-old human embryos were obtained from elective abortions. The neuroretinas were kept in culture as full-thickness sheets for 7-42 days. Results: The control retinas consisted of a neuroblast cell layer and a thin marginal zone. Most explants displayed presence of retinal lamination, but also contained regions of disorganization. Vimentin labeling showed vertically arranged Müller cells in all explants. Recoverin-labeled photoreceptors appeared in explants kept 14 days and longer. By labeling with antibodies against PKC and parvalbumin, rod bipolar cells and amacrine cells could be seen in explants kept for... (More)
Purpose: To study early signs of neuronal and glial differentiation in the human embryonic retina. Materials and Methods: 6.5-to 8-week-old human embryos were obtained from elective abortions. The neuroretinas were kept in culture as full-thickness sheets for 7-42 days. Results: The control retinas consisted of a neuroblast cell layer and a thin marginal zone. Most explants displayed presence of retinal lamination, but also contained regions of disorganization. Vimentin labeling showed vertically arranged Müller cells in all explants. Recoverin-labeled photoreceptors appeared in explants kept 14 days and longer. By labeling with antibodies against PKC and parvalbumin, rod bipolar cells and amacrine cells could be seen in explants kept for 42 days in culture.Conclusions: We have shown that the embryonic full-thickness neuroretina can survive in a culture environment for at least 6 weeks, and can develop several types of the retina-specific neuronal and glial cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Eye Research
volume
33
issue
2
pages
185 - 191
publisher
Taylor & Francis
external identifiers
  • pmid:18293190
  • wos:000253509500009
  • scopus:39749147647
ISSN
0271-3683
DOI
10.1080/02713680701843784
language
English
LU publication?
yes
id
8169fa87-cc36-4113-aacd-dcdad771003b (old id 1041610)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18293190?dopt=Abstract
date added to LUP
2008-03-04 10:51:58
date last changed
2017-01-01 07:37:10
@article{8169fa87-cc36-4113-aacd-dcdad771003b,
  abstract     = {Purpose: To study early signs of neuronal and glial differentiation in the human embryonic retina. Materials and Methods: 6.5-to 8-week-old human embryos were obtained from elective abortions. The neuroretinas were kept in culture as full-thickness sheets for 7-42 days. Results: The control retinas consisted of a neuroblast cell layer and a thin marginal zone. Most explants displayed presence of retinal lamination, but also contained regions of disorganization. Vimentin labeling showed vertically arranged Müller cells in all explants. Recoverin-labeled photoreceptors appeared in explants kept 14 days and longer. By labeling with antibodies against PKC and parvalbumin, rod bipolar cells and amacrine cells could be seen in explants kept for 42 days in culture.Conclusions: We have shown that the embryonic full-thickness neuroretina can survive in a culture environment for at least 6 weeks, and can develop several types of the retina-specific neuronal and glial cells.},
  author       = {Engelsberg, Karl and Ehinger, Berndt and Ghosh, Fredrik},
  issn         = {0271-3683},
  language     = {eng},
  number       = {2},
  pages        = {185--191},
  publisher    = {Taylor & Francis},
  series       = {Current Eye Research},
  title        = {Early development of retinal subtypes in long-term cultures of human embryonic retina.},
  url          = {http://dx.doi.org/10.1080/02713680701843784},
  volume       = {33},
  year         = {2008},
}