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A common idiotype in IgE and its relation to recognition of the grass pollen allergen Phl p 2.

Persson, Helena; Flicker, Sabine; Sadegh, Mardjaneh Karbalaei; Greiff, Lennart LU ; Valenta, Rudolf and Ohlin, Mats LU (2008) In Molecular Immunology 45(9). p.2715-2720
Abstract
The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a... (More)
The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a common idiotype is used to establish specific antibodies with a potential to mediate allergic responses to Phl p 2. Such a restriction may contribute to the limited molecular diversity observed in some IgE populations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Immunology
volume
45
issue
9
pages
2715 - 2720
publisher
Pergamon
external identifiers
  • pmid:18289681
  • wos:000255528400029
  • scopus:40849087700
ISSN
1872-9142
DOI
10.1016/j.molimm.2008.01.004
language
English
LU publication?
yes
id
0d249a7d-9e2f-4501-89a2-16312285e639 (old id 1041685)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18289681?dopt=Abstract
date added to LUP
2008-03-06 13:56:59
date last changed
2017-08-06 03:59:22
@article{0d249a7d-9e2f-4501-89a2-16312285e639,
  abstract     = {The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a common idiotype is used to establish specific antibodies with a potential to mediate allergic responses to Phl p 2. Such a restriction may contribute to the limited molecular diversity observed in some IgE populations.},
  author       = {Persson, Helena and Flicker, Sabine and Sadegh, Mardjaneh Karbalaei and Greiff, Lennart and Valenta, Rudolf and Ohlin, Mats},
  issn         = {1872-9142},
  language     = {eng},
  number       = {9},
  pages        = {2715--2720},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {A common idiotype in IgE and its relation to recognition of the grass pollen allergen Phl p 2.},
  url          = {http://dx.doi.org/10.1016/j.molimm.2008.01.004},
  volume       = {45},
  year         = {2008},
}