A common idiotype in IgE and its relation to recognition of the grass pollen allergen Phl p 2.
Persson, Helena ; Flicker, Sabine ; Sadegh, Mardjaneh Karbalaei ; Greiff, Lennart LU ; Valenta, Rudolf and Ohlin, Mats LU
(2008)
In Molecular Immunology
45(9).
p.2715-2720
- Abstract
- The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a... (More)
- The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a common idiotype is used to establish specific antibodies with a potential to mediate allergic responses to Phl p 2. Such a restriction may contribute to the limited molecular diversity observed in some IgE populations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1041685
- author
- Persson, Helena
; Flicker, Sabine
; Sadegh, Mardjaneh Karbalaei
; Greiff, Lennart
LU
; Valenta, Rudolf
and Ohlin, Mats
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Immunology
- volume
- 45
- issue
- 9
- pages
- 2715 - 2720
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- pmid:18289681
- wos:000255528400029
- scopus:40849087700
- pmid:18289681
- ISSN
- 1872-9142
- DOI
- 10.1016/j.molimm.2008.01.004
- project
- Human IgE repertoires and an anti-allergome resource
- language
- English
- LU publication?
- yes
- id
- 0d249a7d-9e2f-4501-89a2-16312285e639 (old id 1041685)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18289681?dopt=Abstract
- date added to LUP
- 2016-04-01 13:26:10
- date last changed
- 2022-01-27 19:08:46
@article{0d249a7d-9e2f-4501-89a2-16312285e639,
abstract = {{The variable regions of allergen-specific IgE, the isotype mediating allergic responses, are poorly defined to date. In this study we define the character of human antibody binding sites recognizing Phl p 2, a major allergen from timothy grass pollen. Independently raised specificities developed by phage display technology tended to have common sequence motifs (idiotypes), such as IGHV4-31 germline gene origin and heavy chain complementarity-determining region (CDR) 3 length and sequence. They also combined with highly related light chain sequences. Such heavy chain variable domain-encoding transcripts have also been found in the IgE-encoding transcriptome of yet other grass pollen allergic subjects. Altogether these data argue that a common idiotype is used to establish specific antibodies with a potential to mediate allergic responses to Phl p 2. Such a restriction may contribute to the limited molecular diversity observed in some IgE populations.}},
author = {{Persson, Helena and Flicker, Sabine and Sadegh, Mardjaneh Karbalaei and Greiff, Lennart and Valenta, Rudolf and Ohlin, Mats}},
issn = {{1872-9142}},
language = {{eng}},
number = {{9}},
pages = {{2715--2720}},
publisher = {{Pergamon Press Ltd.}},
series = {{Molecular Immunology}},
title = {{A common idiotype in IgE and its relation to recognition of the grass pollen allergen Phl p 2.}},
url = {{http://dx.doi.org/10.1016/j.molimm.2008.01.004}},
doi = {{10.1016/j.molimm.2008.01.004}},
volume = {{45}},
year = {{2008}},
}