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DRhoGEF2 and Diaphanous Regulate Contractile Force during Segmental Groove Morphogenesis in the Drosophila Embryo.

Mulinari, Shai LU ; Padash, Mojgan LU and Häcker, Udo LU (2008) In Molecular Biology of the Cell 19. p.1883-1892
Abstract
Monitoring Editor: Marianne Bronner-Fraser Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement of the Actin cytoskeleton mediated by small GTPases of the Rho family. These GTPases act as molecular switches that are activated by guanine nucleotide exchange factors. One of these factors, DRhoGEF2, plays an important role in the constriction of Actin filaments during pole cell formation, blastoderm cellularization and invagination of the germlayers. Here we show that DRhoGEF2 is equally important during morphogenesis of segmental grooves, which become apparent as tissue infoldings during midembryogenesis. Examination of DRhoGEF2-mutant embryos indicates a role for DRhoGEF2 in the control of cell shape changes... (More)
Monitoring Editor: Marianne Bronner-Fraser Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement of the Actin cytoskeleton mediated by small GTPases of the Rho family. These GTPases act as molecular switches that are activated by guanine nucleotide exchange factors. One of these factors, DRhoGEF2, plays an important role in the constriction of Actin filaments during pole cell formation, blastoderm cellularization and invagination of the germlayers. Here we show that DRhoGEF2 is equally important during morphogenesis of segmental grooves, which become apparent as tissue infoldings during midembryogenesis. Examination of DRhoGEF2-mutant embryos indicates a role for DRhoGEF2 in the control of cell shape changes during segmental groove morphogenesis. Overexpression of DRhoGEF2 in the ectoderm recruits Myosin II to the cell cortex and induces cell contraction. At groove regression DRhoGEF2 is enriched in cells posterior to the groove that undergo apical constriction indicating that groove regression is an active process. We further show that the Formin Diaphanous is required for groove formation and strengthens cell junctions in the epidermis. Morphological analysis suggests that Dia regulates cell shape in a way distinct from DRhoGEF2. We propose that DRhoGEF2 acts through Rho1 to regulate acto-myosin constriction but not Diaphanous-mediated F-Actin nucleation during segmental groove morphogenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Biology of the Cell
volume
19
pages
1883 - 1892
publisher
American Society for Cell Biology
external identifiers
  • pmid:18287521
  • wos:000258952000007
  • scopus:48249086098
ISSN
1939-4586
DOI
10.1091/mbc.E07-12-1230
language
English
LU publication?
yes
id
dc63ae5f-d04c-41b3-9ccb-7adc7351b80f (old id 1041694)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18287521?dopt=Abstract
date added to LUP
2008-03-03 14:55:04
date last changed
2017-01-01 07:41:33
@article{dc63ae5f-d04c-41b3-9ccb-7adc7351b80f,
  abstract     = {Monitoring Editor: Marianne Bronner-Fraser Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement of the Actin cytoskeleton mediated by small GTPases of the Rho family. These GTPases act as molecular switches that are activated by guanine nucleotide exchange factors. One of these factors, DRhoGEF2, plays an important role in the constriction of Actin filaments during pole cell formation, blastoderm cellularization and invagination of the germlayers. Here we show that DRhoGEF2 is equally important during morphogenesis of segmental grooves, which become apparent as tissue infoldings during midembryogenesis. Examination of DRhoGEF2-mutant embryos indicates a role for DRhoGEF2 in the control of cell shape changes during segmental groove morphogenesis. Overexpression of DRhoGEF2 in the ectoderm recruits Myosin II to the cell cortex and induces cell contraction. At groove regression DRhoGEF2 is enriched in cells posterior to the groove that undergo apical constriction indicating that groove regression is an active process. We further show that the Formin Diaphanous is required for groove formation and strengthens cell junctions in the epidermis. Morphological analysis suggests that Dia regulates cell shape in a way distinct from DRhoGEF2. We propose that DRhoGEF2 acts through Rho1 to regulate acto-myosin constriction but not Diaphanous-mediated F-Actin nucleation during segmental groove morphogenesis.},
  author       = {Mulinari, Shai and Padash, Mojgan and Häcker, Udo},
  issn         = {1939-4586},
  language     = {eng},
  pages        = {1883--1892},
  publisher    = {American Society for Cell Biology},
  series       = {Molecular Biology of the Cell},
  title        = {DRhoGEF2 and Diaphanous Regulate Contractile Force during Segmental Groove Morphogenesis in the Drosophila Embryo.},
  url          = {http://dx.doi.org/10.1091/mbc.E07-12-1230},
  volume       = {19},
  year         = {2008},
}