Different affinity of galectins for human serum glycoproteins: galectin-3 binds many protease inhibitors and acute phase proteins.
(2008) In Glycobiology 18(5). p.384-394- Abstract
- Here we report the first survey of galectin binding to glycoproteins of human serum. Serum was subjected to affinity chromatography using immobilized galectins, and the bound glycoproteins were analyzed by electrophoresis, Western blotting, and mass spectrometry. Galectins-3, -8 and -9 bound a much broader range of ligands in serum than previously known, galectin-1 bound less, and galectins-2, -4 and -7 bound only traces or no serum ligands. Galectin-3 bound most major glycoproteins, including alpha-2 macroglobulin and acute phase proteins such as haptoglobin. It bound only a selected minor fraction of transferrin, and bound none or little of IgG. Galectin-8 and -9 bound a similar range of glycoproteins as galectin-3, but in lower amounts,... (More)
- Here we report the first survey of galectin binding to glycoproteins of human serum. Serum was subjected to affinity chromatography using immobilized galectins, and the bound glycoproteins were analyzed by electrophoresis, Western blotting, and mass spectrometry. Galectins-3, -8 and -9 bound a much broader range of ligands in serum than previously known, galectin-1 bound less, and galectins-2, -4 and -7 bound only traces or no serum ligands. Galectin-3 bound most major glycoproteins, including alpha-2 macroglobulin and acute phase proteins such as haptoglobin. It bound only a selected minor fraction of transferrin, and bound none or little of IgG. Galectin-8 and -9 bound a similar range of glycoproteins as galectin-3, but in lower amounts, and galectin-8 had a relative preference for IgA. Galectin-1 bound mainly a fraction of alpha-2 macroglobulin and only traces of other glycoproteins. The binding of galectin-3 to serum glycoproteins requires affinity for LacNAc, since a mutant (R186S), which has lost this affinity, did not bind any serum glycoproteins. The average affinity of galectin-3 for serum glycoproteins was estimated to correspond to K(d) approximately 1-5 muM by modelling of the affinity chromatography and a fluorescence anisotropy assay. Since galectins are expressed on endothelial cells and other cells exposed to serum components, this report gives new insight into function of galectins and the role of their different fine specificity giving differential binding to the serum glycoproteins. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1042086
- author
- Cederfur, Cecilia LU ; Salomonsson, Emma LU ; Nilsson, Jonas ; Halim, Adnan ; Öberg, Christopher LU ; Larson, Göran ; Nilsson, Ulf LU and Leffler, Hakon LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Glycobiology
- volume
- 18
- issue
- 5
- pages
- 384 - 394
- publisher
- Oxford University Press
- external identifiers
-
- pmid:18263896
- wos:000255151000004
- scopus:42449153703
- pmid:18263896
- ISSN
- 1460-2423
- DOI
- 10.1093/glycob/cwn015
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Microbiology, Immunology and Glycobiology - MIG (013025200), Organic chemistry (S/LTH) (011001240)
- id
- 6facd44b-296d-4bcc-a843-d6e1c595163f (old id 1042086)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18263896?dopt=Abstract
- date added to LUP
- 2016-04-01 13:10:09
- date last changed
- 2022-01-27 17:45:22
@article{6facd44b-296d-4bcc-a843-d6e1c595163f,
abstract = {{Here we report the first survey of galectin binding to glycoproteins of human serum. Serum was subjected to affinity chromatography using immobilized galectins, and the bound glycoproteins were analyzed by electrophoresis, Western blotting, and mass spectrometry. Galectins-3, -8 and -9 bound a much broader range of ligands in serum than previously known, galectin-1 bound less, and galectins-2, -4 and -7 bound only traces or no serum ligands. Galectin-3 bound most major glycoproteins, including alpha-2 macroglobulin and acute phase proteins such as haptoglobin. It bound only a selected minor fraction of transferrin, and bound none or little of IgG. Galectin-8 and -9 bound a similar range of glycoproteins as galectin-3, but in lower amounts, and galectin-8 had a relative preference for IgA. Galectin-1 bound mainly a fraction of alpha-2 macroglobulin and only traces of other glycoproteins. The binding of galectin-3 to serum glycoproteins requires affinity for LacNAc, since a mutant (R186S), which has lost this affinity, did not bind any serum glycoproteins. The average affinity of galectin-3 for serum glycoproteins was estimated to correspond to K(d) approximately 1-5 muM by modelling of the affinity chromatography and a fluorescence anisotropy assay. Since galectins are expressed on endothelial cells and other cells exposed to serum components, this report gives new insight into function of galectins and the role of their different fine specificity giving differential binding to the serum glycoproteins.}},
author = {{Cederfur, Cecilia and Salomonsson, Emma and Nilsson, Jonas and Halim, Adnan and Öberg, Christopher and Larson, Göran and Nilsson, Ulf and Leffler, Hakon}},
issn = {{1460-2423}},
language = {{eng}},
number = {{5}},
pages = {{384--394}},
publisher = {{Oxford University Press}},
series = {{Glycobiology}},
title = {{Different affinity of galectins for human serum glycoproteins: galectin-3 binds many protease inhibitors and acute phase proteins.}},
url = {{http://dx.doi.org/10.1093/glycob/cwn015}},
doi = {{10.1093/glycob/cwn015}},
volume = {{18}},
year = {{2008}},
}