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Influence of Ethanol on Lipid Membranes: From Lateral Pressure Profiles to Dynamics and Partitioning.

Terama, Emma; Ollila, O; Salonen, Emppu; Rowat, Amy; Trandum, Christa; Westh, Peter; Patra, Michael LU ; Karttunen, Mikko and Vattulainen, Ilpo (2008) In The Journal of Physical Chemistry Part B 112(13). p.4131-4139
Abstract
We have combined experiments with atomic-scale molecular dynamics simulations to consider the influence of ethanol on a variety of lipid membrane properties. We first employed isothermal titration calorimetry together with the solvent-null method to study the partitioning of ethanol molecules into saturated and unsaturated membrane systems. The results show that ethanol partitioning is considerably more favorable in unsaturated bilayers, which are characterized by their more disordered nature compared to their saturated counterparts. Simulation studies at varying ethanol concentrations propose that the partitioning of ethanol depends on its concentration, implying that the partitioning is a nonideal process. To gain further insight into... (More)
We have combined experiments with atomic-scale molecular dynamics simulations to consider the influence of ethanol on a variety of lipid membrane properties. We first employed isothermal titration calorimetry together with the solvent-null method to study the partitioning of ethanol molecules into saturated and unsaturated membrane systems. The results show that ethanol partitioning is considerably more favorable in unsaturated bilayers, which are characterized by their more disordered nature compared to their saturated counterparts. Simulation studies at varying ethanol concentrations propose that the partitioning of ethanol depends on its concentration, implying that the partitioning is a nonideal process. To gain further insight into the permeation of alcohols and their influence on lipid dynamics, we also employed molecular dynamics simulations to quantify kinetic events associated with the permeation of alcohols across a membrane, and to characterize the rotational and lateral diffusion of lipids and alcohols in these systems. The simulation results are in agreement with available experimental data and further show that alcohols have a small but non-vanishing effect on the dynamics of lipids in a membrane. The influence of ethanol on the lateral pressure profile of a lipid bilayer is found to be prominent: ethanol reduces the tension at the membrane-water interface and reduces the peaks in the lateral pressure profile close to the membrane-water interface. The changes in the lateral pressure profile are several hundred atmospheres. This supports the hypothesis that anesthetics may act by changing the lateral pressure profile exerted on proteins embedded in membranes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Part B
volume
112
issue
13
pages
4131 - 4139
publisher
The American Chemical Society
external identifiers
  • pmid:18341314
  • wos:000254540900032
  • scopus:42649132203
ISSN
1520-5207
DOI
10.1021/jp0750811
language
English
LU publication?
yes
id
4f4a7ec1-7c76-45a1-a637-b2068ed1aa77 (old id 1052465)
date added to LUP
2008-04-28 21:15:41
date last changed
2017-08-13 04:06:23
@article{4f4a7ec1-7c76-45a1-a637-b2068ed1aa77,
  abstract     = {We have combined experiments with atomic-scale molecular dynamics simulations to consider the influence of ethanol on a variety of lipid membrane properties. We first employed isothermal titration calorimetry together with the solvent-null method to study the partitioning of ethanol molecules into saturated and unsaturated membrane systems. The results show that ethanol partitioning is considerably more favorable in unsaturated bilayers, which are characterized by their more disordered nature compared to their saturated counterparts. Simulation studies at varying ethanol concentrations propose that the partitioning of ethanol depends on its concentration, implying that the partitioning is a nonideal process. To gain further insight into the permeation of alcohols and their influence on lipid dynamics, we also employed molecular dynamics simulations to quantify kinetic events associated with the permeation of alcohols across a membrane, and to characterize the rotational and lateral diffusion of lipids and alcohols in these systems. The simulation results are in agreement with available experimental data and further show that alcohols have a small but non-vanishing effect on the dynamics of lipids in a membrane. The influence of ethanol on the lateral pressure profile of a lipid bilayer is found to be prominent: ethanol reduces the tension at the membrane-water interface and reduces the peaks in the lateral pressure profile close to the membrane-water interface. The changes in the lateral pressure profile are several hundred atmospheres. This supports the hypothesis that anesthetics may act by changing the lateral pressure profile exerted on proteins embedded in membranes.},
  author       = {Terama, Emma and Ollila, O and Salonen, Emppu and Rowat, Amy and Trandum, Christa and Westh, Peter and Patra, Michael and Karttunen, Mikko and Vattulainen, Ilpo},
  issn         = {1520-5207},
  language     = {eng},
  number       = {13},
  pages        = {4131--4139},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Part B},
  title        = {Influence of Ethanol on Lipid Membranes: From Lateral Pressure Profiles to Dynamics and Partitioning.},
  url          = {http://dx.doi.org/10.1021/jp0750811},
  volume       = {112},
  year         = {2008},
}