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Association between polymorphisms in the prostate-specific antigen (PSA) promoter and release of PSA.

Sävblom, Charlotta LU ; Giwercman, Aleksander LU ; Malm, Johan LU ; Halldén, Christer LU ; Lundin, Kristina LU ; Lilja, Hans LU and Giwercman, Yvonne LU (2009) In International journal of andrology 32. p.479-485
Abstract
Variations in serum prostate-specific antigen (PSA) have been ascribed to A/G nucleotide polymorphisms located at -158 bp (rs266882) and -4643 bp (rs925013), relative to the transcription start site within the promoter of the PSA gene. PSA is also an androgen receptor target (AR) gene and polymorphisms in AR gene are known to affect AR function. Our objective was to compare the impact of these A/G polymorphisms separately or in combination with AR CAG micro satellite on regulation of PSA secretion into seminal plasma and blood in young men. Leukocyte DNA was extracted from 291 conscripts and genotyping performed with the Sequenom Mass Array System. PSA was measured with an immunofluorometric assay. Linear regression analysis was used to... (More)
Variations in serum prostate-specific antigen (PSA) have been ascribed to A/G nucleotide polymorphisms located at -158 bp (rs266882) and -4643 bp (rs925013), relative to the transcription start site within the promoter of the PSA gene. PSA is also an androgen receptor target (AR) gene and polymorphisms in AR gene are known to affect AR function. Our objective was to compare the impact of these A/G polymorphisms separately or in combination with AR CAG micro satellite on regulation of PSA secretion into seminal plasma and blood in young men. Leukocyte DNA was extracted from 291 conscripts and genotyping performed with the Sequenom Mass Array System. PSA was measured with an immunofluorometric assay. Linear regression analysis was used to test the association of polymorphism frequencies with serum and seminal plasma levels of PSA. PSA gene polymorphisms at -158 bp or -4643 bp did not alone influence total PSA (tPSA) levels in seminal plasma or in blood. Homozygotes for the A-allele at -158 bp in combination with CAG > 22 had significantly higher serum levels of tPSA than subjects carrying the G-allele (p = 0.01). In conclusion, the PSA gene polymorphisms did not importantly influence the levels of tPSA in seminal plasma or in blood. tPSA in serum was influenced by interactions between PSA promoter variants and AR CAG polymorphism. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International journal of andrology
volume
32
pages
479 - 485
publisher
Wiley-Blackwell
external identifiers
  • pmid:18336535
  • wos:000269591000007
  • scopus:70049118833
ISSN
1365-2605
DOI
10.1111/j.1365-2605.2008.00882.x
language
English
LU publication?
yes
id
2cf27354-3378-4b0c-b8dd-5507bc9e223d (old id 1052520)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18336535?dopt=Abstract
date added to LUP
2008-04-03 07:48:42
date last changed
2017-01-01 07:50:37
@article{2cf27354-3378-4b0c-b8dd-5507bc9e223d,
  abstract     = {Variations in serum prostate-specific antigen (PSA) have been ascribed to A/G nucleotide polymorphisms located at -158 bp (rs266882) and -4643 bp (rs925013), relative to the transcription start site within the promoter of the PSA gene. PSA is also an androgen receptor target (AR) gene and polymorphisms in AR gene are known to affect AR function. Our objective was to compare the impact of these A/G polymorphisms separately or in combination with AR CAG micro satellite on regulation of PSA secretion into seminal plasma and blood in young men. Leukocyte DNA was extracted from 291 conscripts and genotyping performed with the Sequenom Mass Array System. PSA was measured with an immunofluorometric assay. Linear regression analysis was used to test the association of polymorphism frequencies with serum and seminal plasma levels of PSA. PSA gene polymorphisms at -158 bp or -4643 bp did not alone influence total PSA (tPSA) levels in seminal plasma or in blood. Homozygotes for the A-allele at -158 bp in combination with CAG > 22 had significantly higher serum levels of tPSA than subjects carrying the G-allele (p = 0.01). In conclusion, the PSA gene polymorphisms did not importantly influence the levels of tPSA in seminal plasma or in blood. tPSA in serum was influenced by interactions between PSA promoter variants and AR CAG polymorphism.},
  author       = {Sävblom, Charlotta and Giwercman, Aleksander and Malm, Johan and Halldén, Christer and Lundin, Kristina and Lilja, Hans and Giwercman, Yvonne},
  issn         = {1365-2605},
  language     = {eng},
  pages        = {479--485},
  publisher    = {Wiley-Blackwell},
  series       = {International journal of andrology},
  title        = {Association between polymorphisms in the prostate-specific antigen (PSA) promoter and release of PSA.},
  url          = {http://dx.doi.org/10.1111/j.1365-2605.2008.00882.x},
  volume       = {32},
  year         = {2009},
}