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Monoclonal Behavior of Molecularly Imprinted Polymer Nanoparticles in Capillary Electrochromatography.

Priego-Capote, Feliciano LU ; Ye, Lei LU orcid ; Shakil, Sadia ; Shamsi, Shahab and Nilsson, Staffan LU (2008) In Analytical Chemistry 80. p.2881-2887
Abstract
A new approach based on miniemulsion polymerization is demonstrated for synthesis of molecularly imprinted nanoparticles (MIP-NP; 30-150 nm) with "monoclonal" binding behavior. The performance of the MIP nanoparticles is characterized with partial filling capillary electrochromatography, for the analysis of rac-propranolol, where (S)-propranolol is used as a template. In contrast to previous HPLC and CEC methods based on the use of MIPs, there is no apparent tailing for the enantiomer peaks, and baseline separation with 25 000-60 000 plate number is achieved. These effects are attributed to reduction of the MIP site heterogeneity by means of peripheral location of the core cross-linked NP and to MIP-binding sites with the same ordered... (More)
A new approach based on miniemulsion polymerization is demonstrated for synthesis of molecularly imprinted nanoparticles (MIP-NP; 30-150 nm) with "monoclonal" binding behavior. The performance of the MIP nanoparticles is characterized with partial filling capillary electrochromatography, for the analysis of rac-propranolol, where (S)-propranolol is used as a template. In contrast to previous HPLC and CEC methods based on the use of MIPs, there is no apparent tailing for the enantiomer peaks, and baseline separation with 25 000-60 000 plate number is achieved. These effects are attributed to reduction of the MIP site heterogeneity by means of peripheral location of the core cross-linked NP and to MIP-binding sites with the same ordered radial orientation. This new MIP approach is based on the substitution of the functional monomers with a surfactant monomer, sodium N-undecenoyl glycinate (SUG) for improved inclusion in the MIP-NP structure and to the use of a miniemulsion in the MIP-NP synthesis. The feasibility of working primarily with aqueous electrolytes (10 mM phosphate with a 20% acetonitrile at pH 7) is attributable to the micellar character of the MIP-NPs, provided by the inclusion of the SUG monomers in the structure. To our knowledge this is the first example of "monoclonal" MIP-NPs incorporated in CEC separations of drug enantiomers. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
80
pages
2881 - 2887
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:18336010
  • wos:000254969100031
  • scopus:42349106292
ISSN
1520-6882
DOI
10.1021/ac070038v
language
English
LU publication?
yes
id
dc4f75bd-8666-4190-bfb2-859c7d90f89d (old id 1052535)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18336010?dopt=Abstract
date added to LUP
2016-04-04 07:54:33
date last changed
2022-04-15 19:36:29
@article{dc4f75bd-8666-4190-bfb2-859c7d90f89d,
  abstract     = {{A new approach based on miniemulsion polymerization is demonstrated for synthesis of molecularly imprinted nanoparticles (MIP-NP; 30-150 nm) with "monoclonal" binding behavior. The performance of the MIP nanoparticles is characterized with partial filling capillary electrochromatography, for the analysis of rac-propranolol, where (S)-propranolol is used as a template. In contrast to previous HPLC and CEC methods based on the use of MIPs, there is no apparent tailing for the enantiomer peaks, and baseline separation with 25 000-60 000 plate number is achieved. These effects are attributed to reduction of the MIP site heterogeneity by means of peripheral location of the core cross-linked NP and to MIP-binding sites with the same ordered radial orientation. This new MIP approach is based on the substitution of the functional monomers with a surfactant monomer, sodium N-undecenoyl glycinate (SUG) for improved inclusion in the MIP-NP structure and to the use of a miniemulsion in the MIP-NP synthesis. The feasibility of working primarily with aqueous electrolytes (10 mM phosphate with a 20% acetonitrile at pH 7) is attributable to the micellar character of the MIP-NPs, provided by the inclusion of the SUG monomers in the structure. To our knowledge this is the first example of "monoclonal" MIP-NPs incorporated in CEC separations of drug enantiomers.}},
  author       = {{Priego-Capote, Feliciano and Ye, Lei and Shakil, Sadia and Shamsi, Shahab and Nilsson, Staffan}},
  issn         = {{1520-6882}},
  language     = {{eng}},
  pages        = {{2881--2887}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Analytical Chemistry}},
  title        = {{Monoclonal Behavior of Molecularly Imprinted Polymer Nanoparticles in Capillary Electrochromatography.}},
  url          = {{http://dx.doi.org/10.1021/ac070038v}},
  doi          = {{10.1021/ac070038v}},
  volume       = {{80}},
  year         = {{2008}},
}