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NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae.

Almeida, Joao LU ; Röder, Anja; Modig, Tobias LU ; Laadan, Boaz LU ; Lidén, Gunnar LU and Gorwa-Grauslund, Marie-Francoise LU (2008) In Applied Microbiology and Biotechnology 78(6). p.939-945
Abstract
Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed... (More)
Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate. (Less)
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author
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type
Contribution to journal
publication status
published
subject
keywords
ADH1 - ADH6 - Hydroxymethyl furfural - Saccharomyces cerevisiae - Lignocellulosic hydrolysate
in
Applied Microbiology and Biotechnology
volume
78
issue
6
pages
939 - 945
publisher
Springer
external identifiers
  • pmid:18330568
  • wos:000254258500003
  • scopus:43349084131
ISSN
1432-0614
DOI
10.1007/s00253-008-1364-y
language
English
LU publication?
yes
id
d4260d05-60e6-4839-8828-b4a6d93c6c18 (old id 1052694)
date added to LUP
2008-04-29 09:46:36
date last changed
2017-11-19 03:54:12
@article{d4260d05-60e6-4839-8828-b4a6d93c6c18,
  abstract     = {Saccharomyces cerevisiae alcohol dehydrogenases responsible for NADH-, and NADPH-specific reduction of the furaldehydes 5-hydroxymethyl-furfural (HMF) and furfural have previously been identified. In the present study, strains overexpressing the corresponding genes (mut-ADH1 and ADH6), together with a control strain, were compared in defined medium for anaerobic fermentation of glucose in the presence and absence of HMF. All strains showed a similar fermentation pattern in the absence of HMF. In the presence of HMF, the strain overexpressing ADH6 showed the highest HMF reduction rate and the highest specific ethanol productivity, followed by the strain overexpressing mut-ADH1. This correlated with in vitro HMF reduction capacity observed in the ADH6 overexpressing strain. Acetate and glycerol yields per biomass increased considerably in the ADH6 strain. In the other two strains, only the overall acetate yield per biomass was affected. When compared in batch fermentation of spruce hydrolysate, strains overexpressing ADH6 and mut-ADH1 had five times higher HMF uptake rate than the control strain and improved specific ethanol productivity. Overall, our results demonstrate that (1) the cofactor usage in the HMF reduction affects the product distribution, and (2) increased HMF reduction activity results in increased specific ethanol productivity in defined mineral medium and in spruce hydrolysate.},
  author       = {Almeida, Joao and Röder, Anja and Modig, Tobias and Laadan, Boaz and Lidén, Gunnar and Gorwa-Grauslund, Marie-Francoise},
  issn         = {1432-0614},
  keyword      = {ADH1 -  ADH6  - Hydroxymethyl furfural -  Saccharomyces cerevisiae  - Lignocellulosic hydrolysate},
  language     = {eng},
  number       = {6},
  pages        = {939--945},
  publisher    = {Springer},
  series       = {Applied Microbiology and Biotechnology},
  title        = {NADH- vs NADPH-coupled reduction of 5-hydroxymethyl furfural (HMF) and its implications on product distribution in Saccharomyces cerevisiae.},
  url          = {http://dx.doi.org/10.1007/s00253-008-1364-y},
  volume       = {78},
  year         = {2008},
}