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Induction of Vascular Remodeling in the Lung by Chronic House Dust Mite Exposure.

Rydell-Törmänen, Kristina LU ; Johnson, Jill R; Fattouh, Ramzi; Jordana, Manel and Erjefält, Jonas LU (2008) In American Journal of Respiratory Cell and Molecular Biology 39. p.61-67
Abstract
Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were... (More)
Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were exposed to HDM intranasally for 7 or 20 consecutive weeks, and resolution of the inflammatory and remodeling response to allergen was investigated 4 weeks following the end of a 7-week exposure protocol. Measures of vascular remodeling, including total collagen deposition, procollagen I-production, endothelial and smooth muscle cell proliferation, smooth muscle area and presence of myofibroblasts were investigated histologically in lung vessels of different sizes and locations. We observed an increase in total collagen content which did not resolve upon cessation of allergen exposure. Other parameters were significantly increased following 7 and/or 20 weeks of allergen exposure, but returned to baseline following allergen withdrawal. We conclude that respiratory HDM exposure induces not only airway remodeling, but also pulmonary vascular remodeling, and in accordance with airway remodeling, some components of these structural changes may be irreversible. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Respiratory Cell and Molecular Biology
volume
39
pages
61 - 67
publisher
American Thoracic Society
external identifiers
  • pmid:18314535
  • wos:000257037600008
  • scopus:45849114330
ISSN
1535-4989
DOI
10.1165/rcmb.2007-0441OC
language
English
LU publication?
yes
id
4cacf7c1-ebc7-4b2b-ada3-8b8641dc3a06 (old id 1052861)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/18314535?dopt=Abstract
date added to LUP
2008-04-02 16:54:03
date last changed
2017-09-17 08:17:31
@article{4cacf7c1-ebc7-4b2b-ada3-8b8641dc3a06,
  abstract     = {Structural changes to the lung are known to be associated with chronic asthma. In addition to the well-described alterations to the airway wall, asthma is also associated with vascular modifications, although this aspect of remodeling is poorly understood. We therefore sought to evaluate the character and kinetics of vascular remodeling in response to chronic aeroallergen exposure. However, since many OVA-driven models used to investigate allergic airway disease do so in the absence of persistent airway inflammation, we chose instead to employ a protocol of chronic respiratory exposure to house dust mite extract (HDM), which has been shown to induce persistent airway inflammation consistent with that seen in human asthmatics. Mice were exposed to HDM intranasally for 7 or 20 consecutive weeks, and resolution of the inflammatory and remodeling response to allergen was investigated 4 weeks following the end of a 7-week exposure protocol. Measures of vascular remodeling, including total collagen deposition, procollagen I-production, endothelial and smooth muscle cell proliferation, smooth muscle area and presence of myofibroblasts were investigated histologically in lung vessels of different sizes and locations. We observed an increase in total collagen content which did not resolve upon cessation of allergen exposure. Other parameters were significantly increased following 7 and/or 20 weeks of allergen exposure, but returned to baseline following allergen withdrawal. We conclude that respiratory HDM exposure induces not only airway remodeling, but also pulmonary vascular remodeling, and in accordance with airway remodeling, some components of these structural changes may be irreversible.},
  author       = {Rydell-Törmänen, Kristina and Johnson, Jill R and Fattouh, Ramzi and Jordana, Manel and Erjefält, Jonas},
  issn         = {1535-4989},
  language     = {eng},
  pages        = {61--67},
  publisher    = {American Thoracic Society},
  series       = {American Journal of Respiratory Cell and Molecular Biology},
  title        = {Induction of Vascular Remodeling in the Lung by Chronic House Dust Mite Exposure.},
  url          = {http://dx.doi.org/10.1165/rcmb.2007-0441OC},
  volume       = {39},
  year         = {2008},
}