PITX2 gain-of-function induced defects in mouse forelimb development.
(2008) In BMC Developmental Biology 8.- Abstract
- BACKGROUND: Limb development and patterning originate from a complex interplay between the skeletal elements, tendons, and muscles of the limb. One of the genes involved in patterning of limb muscles is the homeobox transcription factor Pitx2 but its role in forelimb development is uncharacterized. Pitx2 is expressed in the majority of premature presumptive forelimb musculature at embryonic day 12.5 and then maintained throughout embryogenesis to adult skeletal muscle. RESULTS: To further study the role of Pitx2 in forelimb development we have generated transgenic mice that exhibit a pulse of PITX2 over-expression at embryonic day 13.5 and 14.5 in the developing forelimb mesenchyme. These mice exhibit a distal misplacement of the biceps... (More)
- BACKGROUND: Limb development and patterning originate from a complex interplay between the skeletal elements, tendons, and muscles of the limb. One of the genes involved in patterning of limb muscles is the homeobox transcription factor Pitx2 but its role in forelimb development is uncharacterized. Pitx2 is expressed in the majority of premature presumptive forelimb musculature at embryonic day 12.5 and then maintained throughout embryogenesis to adult skeletal muscle. RESULTS: To further study the role of Pitx2 in forelimb development we have generated transgenic mice that exhibit a pulse of PITX2 over-expression at embryonic day 13.5 and 14.5 in the developing forelimb mesenchyme. These mice exhibit a distal misplacement of the biceps brachii insertion during embryogenesis, which twists the forelimb musculature resulting in severe skeletal malformations. The skeletal malformations have some similarities to the forearm deformities present in Leri-Weill dyschondrosteosis. CONCLUSION: Taken together, the tendon, muscle, and bone anomalies further support a role of Pitx2 in forelimb development and may also shed light on the interaction between the skeletal elements and muscles of the limb during embryogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1052888
- author
- Holmberg, Johan K LU ; Ingner, Gorel ; Johansson, Curt R ; Leander, Peter LU and Hjalt, Tord LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BMC Developmental Biology
- volume
- 8
- article number
- 25
- publisher
- BioMed Central (BMC)
- external identifiers
-
- pmid:18312615
- wos:000254282200001
- scopus:43149111060
- ISSN
- 1471-213X
- DOI
- 10.1186/1471-213X-8-25
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Cell and Matrix Biology (LUR000002), Neuronal Survival (013212041), Department of Clinical Sciences, Malmö (013240000), Muscle biology (013212015), Medical Radiology Unit (013241410)
- id
- 3e002902-cfba-4b7a-b3cd-62b7878eed35 (old id 1052888)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18312615?dopt=Abstract
- date added to LUP
- 2016-04-04 09:18:45
- date last changed
- 2022-01-29 17:16:56
@article{3e002902-cfba-4b7a-b3cd-62b7878eed35, abstract = {{BACKGROUND: Limb development and patterning originate from a complex interplay between the skeletal elements, tendons, and muscles of the limb. One of the genes involved in patterning of limb muscles is the homeobox transcription factor Pitx2 but its role in forelimb development is uncharacterized. Pitx2 is expressed in the majority of premature presumptive forelimb musculature at embryonic day 12.5 and then maintained throughout embryogenesis to adult skeletal muscle. RESULTS: To further study the role of Pitx2 in forelimb development we have generated transgenic mice that exhibit a pulse of PITX2 over-expression at embryonic day 13.5 and 14.5 in the developing forelimb mesenchyme. These mice exhibit a distal misplacement of the biceps brachii insertion during embryogenesis, which twists the forelimb musculature resulting in severe skeletal malformations. The skeletal malformations have some similarities to the forearm deformities present in Leri-Weill dyschondrosteosis. CONCLUSION: Taken together, the tendon, muscle, and bone anomalies further support a role of Pitx2 in forelimb development and may also shed light on the interaction between the skeletal elements and muscles of the limb during embryogenesis.}}, author = {{Holmberg, Johan K and Ingner, Gorel and Johansson, Curt R and Leander, Peter and Hjalt, Tord}}, issn = {{1471-213X}}, language = {{eng}}, publisher = {{BioMed Central (BMC)}}, series = {{BMC Developmental Biology}}, title = {{PITX2 gain-of-function induced defects in mouse forelimb development.}}, url = {{http://dx.doi.org/10.1186/1471-213X-8-25}}, doi = {{10.1186/1471-213X-8-25}}, volume = {{8}}, year = {{2008}}, }