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A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.

Leandersson, Karin LU ; Mårtensson, A; Mertsching, E; Winkler, T; Ceredig, R; Mårtensson, I-L and Ivars, Fredrik LU (2002) In Scandinavian Journal of Immunology 55(2). p.119-128
Abstract
We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction,... (More)
We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Antigen, T-Cell, gamma-delta/metabolism, Receptors, Interleukin-2/*genetics/*metabolism, alpha-beta/metabolism, Transgenic, Mice, Membrane Glycoproteins/*genetics, Liver/cytology/immunology, Hematopoietic Stem Cells/immunology, Genetic Markers, Reporter, Genes, Gene Expression, Fetus/cytology/immunology, B-Lymphocytes/immunology, CD44/metabolism, Animal, Antigens, T-Lymphocyte Subsets/*immunology, Thymus Gland/cytology/immunology
in
Scandinavian Journal of Immunology
volume
55
issue
2
pages
119 - 128
publisher
Wiley-Blackwell
external identifiers
  • wos:000173865700002
  • pmid:11896928
  • scopus:0036010568
ISSN
1365-3083
DOI
10.1046/j.1365-3083.2002.01022.x
language
English
LU publication?
yes
id
be0f0117-3ea6-49b2-9aee-e5fa1d096022 (old id 105980)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11896928&dopt=Abstract
date added to LUP
2007-07-18 16:10:49
date last changed
2017-01-01 06:49:31
@article{be0f0117-3ea6-49b2-9aee-e5fa1d096022,
  abstract     = {We have recently generated 5'lambda5-huTAC mice, which express the human CD25 (huTAC) gene under the control of the 5'-flanking region of the mouse lambda5-gene. The huTAC-transgene was expressed in pre-B cells but neither in mature B cells nor in T cells of these mice. In this report we demonstrate that the transgene is also transiently expressed by adult CD25+ CD3-CD4-CD8- (triple negative, TN) thymocytes and in fetal thymocytes. The huTAC+, in contrast to the huTAC- subpopulation of the CD44+CD25+ TN cells, was unexpectedly found not to express the pTalpha-gene. Still the huTAC+CD44+CD25+ TN cells reconstituted the development of both alphabeta and gammadelta lineage cells equally efficiently as the pTalpha-expressing huTAC- fraction, demonstrating that this pTalpha-negative subpopulation contained precursors for both T-cell lineages. Single cell reverse transcription-polymerase chain reaction (RT-PCR) experiments demonstrated that also in normal mice only a fraction of CD44+CD25+ and CD44-CD25+ TN cells expressed this gene. Taken together, these data indicate that huTAC transgene expression revealed a truly pTalpha-negative fraction of the CD44+CD25+ TN cells. The observation that not all precursors in the CD25+ TN population express the pTalpha-gene has important implications for the understanding of early T-cell development and T-cell lineage commitment.},
  author       = {Leandersson, Karin and Mårtensson, A and Mertsching, E and Winkler, T and Ceredig, R and Mårtensson, I-L and Ivars, Fredrik},
  issn         = {1365-3083},
  keyword      = {Antigen,T-Cell,gamma-delta/metabolism,Receptors,Interleukin-2/*genetics/*metabolism,alpha-beta/metabolism,Transgenic,Mice,Membrane Glycoproteins/*genetics,Liver/cytology/immunology,Hematopoietic Stem Cells/immunology,Genetic Markers,Reporter,Genes,Gene Expression,Fetus/cytology/immunology,B-Lymphocytes/immunology,CD44/metabolism,Animal,Antigens,T-Lymphocyte Subsets/*immunology,Thymus Gland/cytology/immunology},
  language     = {eng},
  number       = {2},
  pages        = {119--128},
  publisher    = {Wiley-Blackwell},
  series       = {Scandinavian Journal of Immunology},
  title        = {A pTalpha-negative subpopulation of CD25+ TN thymocytes revealed by a transgenic marker.},
  url          = {http://dx.doi.org/10.1046/j.1365-3083.2002.01022.x},
  volume       = {55},
  year         = {2002},
}