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Molecularly imprinted CEC sorbents: investigations into polymer preparation and electrolyte composition.

Schweitz, Leif LU ; Andersson, Lars I LU and Nilsson, Staffan LU (2002) In Analyst 127(1). p.22-28
Abstract
The influence of the sorbent preparation protocol and separation parameters on the selectivity and chromatographic efficiency of super-porous molecularly imprinted polymer (MIP) monoliths in capillary electrochromatography (CEC) was studied. Chiral templates were employed and enantiomer separation and resolution were used as measures of imprint selectivity and column efficiency, respectively; the latter was in addition studied by chromatography of non-related aromatic structures. The polymer preparation was varied with respect to monomer composition in the pre-polymerisation mixture and also the use of single versus multiple template(s). The separation parameters investigated were type and content of organic solvent and surfactant modifier... (More)
The influence of the sorbent preparation protocol and separation parameters on the selectivity and chromatographic efficiency of super-porous molecularly imprinted polymer (MIP) monoliths in capillary electrochromatography (CEC) was studied. Chiral templates were employed and enantiomer separation and resolution were used as measures of imprint selectivity and column efficiency, respectively; the latter was in addition studied by chromatography of non-related aromatic structures. The polymer preparation was varied with respect to monomer composition in the pre-polymerisation mixture and also the use of single versus multiple template(s). The separation parameters investigated were type and content of organic solvent and surfactant modifier in the electrolyte. It was found that acetone and acetonitrile in buffer mixtures provided enantiomer separation of enantiomers of the template and also structural analogues; however, the degree of separation was greatly influenced by the content in the electrolyte. Three surfactants, sodium dodecylsulfate (SDS), cetyltrimethylammonium bromide (CTAB) and polyoxyethylene sorbitanmonolaurate (Tween 20), were examined as electrolyte modifiers. It was found that addition of SDS decreased and CTAB and Tween 20 increased the enantiomer separation. SDS and CTAB could be used up to 1 mM concentration whereas Tween could be used up to 90 mM concentration without causing baseline disturbances. The effects found and demonstrated strongly suggest that these parameters are to be considered during optimisation of an MIP-CEC system. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analyst
volume
127
issue
1
pages
22 - 28
publisher
Royal Society of Chemistry
external identifiers
  • wos:000173475200007
  • pmid:11827391
  • scopus:0036141893
ISSN
1364-5528
DOI
10.1039/b105104k
language
English
LU publication?
yes
id
462f0fb2-6b85-46a6-bfff-9b5a9cdbf8ff (old id 106196)
date added to LUP
2007-06-27 15:35:26
date last changed
2017-01-29 04:12:04
@article{462f0fb2-6b85-46a6-bfff-9b5a9cdbf8ff,
  abstract     = {The influence of the sorbent preparation protocol and separation parameters on the selectivity and chromatographic efficiency of super-porous molecularly imprinted polymer (MIP) monoliths in capillary electrochromatography (CEC) was studied. Chiral templates were employed and enantiomer separation and resolution were used as measures of imprint selectivity and column efficiency, respectively; the latter was in addition studied by chromatography of non-related aromatic structures. The polymer preparation was varied with respect to monomer composition in the pre-polymerisation mixture and also the use of single versus multiple template(s). The separation parameters investigated were type and content of organic solvent and surfactant modifier in the electrolyte. It was found that acetone and acetonitrile in buffer mixtures provided enantiomer separation of enantiomers of the template and also structural analogues; however, the degree of separation was greatly influenced by the content in the electrolyte. Three surfactants, sodium dodecylsulfate (SDS), cetyltrimethylammonium bromide (CTAB) and polyoxyethylene sorbitanmonolaurate (Tween 20), were examined as electrolyte modifiers. It was found that addition of SDS decreased and CTAB and Tween 20 increased the enantiomer separation. SDS and CTAB could be used up to 1 mM concentration whereas Tween could be used up to 90 mM concentration without causing baseline disturbances. The effects found and demonstrated strongly suggest that these parameters are to be considered during optimisation of an MIP-CEC system.},
  author       = {Schweitz, Leif and Andersson, Lars I and Nilsson, Staffan},
  issn         = {1364-5528},
  language     = {eng},
  number       = {1},
  pages        = {22--28},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Molecularly imprinted CEC sorbents: investigations into polymer preparation and electrolyte composition.},
  url          = {http://dx.doi.org/10.1039/b105104k},
  volume       = {127},
  year         = {2002},
}