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Recovery of recombinant cutinase using detergent foam

Fernandes, Sheryl; Mattiasson, Bo LU and Hatti-Kaul, Rajni LU (2002) In Biotechnology Progress 18(1). p.116-123
Abstract
Foam generated by vigorous stirring of a nonionic detergent, Triton X-114, was used for the recovery of recombinant cutinase expressed by Saccharomyces cerevisiae. The enzyme with a hydrophobic fusion tag, (Trp-Pro)(4), was recovered with a higher yield as compared to the wild-type cutinase, indicating the involvement of hydrophobic interactions in protein isolation with the foam. The influence of various factors including volume, dilution, pH, different additives, and cell concentration in the medium on enzyme recovery was investigated. Interaction of the enzyme with detergent was monitored using fluorescence spectroscopy. No significant changes in protein conformation after the isolation procedure were observed using circular dichroism.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
18
issue
1
pages
116 - 123
publisher
The American Chemical Society
external identifiers
  • wos:000173819100017
  • scopus:0036008327
ISSN
1520-6033
DOI
10.1021/bp010161m
language
English
LU publication?
yes
id
702388dc-d9c3-4ad2-a98e-2cc646b15713 (old id 106246)
date added to LUP
2007-07-02 10:03:37
date last changed
2017-01-01 07:12:18
@article{702388dc-d9c3-4ad2-a98e-2cc646b15713,
  abstract     = {Foam generated by vigorous stirring of a nonionic detergent, Triton X-114, was used for the recovery of recombinant cutinase expressed by Saccharomyces cerevisiae. The enzyme with a hydrophobic fusion tag, (Trp-Pro)(4), was recovered with a higher yield as compared to the wild-type cutinase, indicating the involvement of hydrophobic interactions in protein isolation with the foam. The influence of various factors including volume, dilution, pH, different additives, and cell concentration in the medium on enzyme recovery was investigated. Interaction of the enzyme with detergent was monitored using fluorescence spectroscopy. No significant changes in protein conformation after the isolation procedure were observed using circular dichroism.},
  author       = {Fernandes, Sheryl and Mattiasson, Bo and Hatti-Kaul, Rajni},
  issn         = {1520-6033},
  language     = {eng},
  number       = {1},
  pages        = {116--123},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Recovery of recombinant cutinase using detergent foam},
  url          = {http://dx.doi.org/10.1021/bp010161m},
  volume       = {18},
  year         = {2002},
}