Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity.
(2002) In Clinical and Diagnostic Laboratory Immunology 9(2). p.287-294- Abstract
- This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and... (More)
- This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106435
- author
- Du, Liping LU ; Fukushima, Sachiko ; Sallmyr, Annahita LU ; Manthorpe, Rolf LU and Bredberg, Anders LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Epitopes, Epithelial Cells/cytology/*immunology, Enzyme-Linked Immunosorbent Assay, DNA Damage, Autoimmune Diseases/diagnosis/immunology, Apoptosis/*immunology, Antigen-Antibody Reactions, Antinuclear/*analysis/immunology, Cultured, Antibodies, Human, Hypertonic Solutions, Osmotic Pressure, Support, Non-U.S. Gov't, Tumor Cells
- in
- Clinical and Diagnostic Laboratory Immunology
- volume
- 9
- issue
- 2
- pages
- 287 - 294
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000174457600012
- pmid:11874865
- scopus:0036126110
- ISSN
- 1071-412X
- DOI
- 10.1128/CDLI.9.2.287-294.2002
- language
- English
- LU publication?
- yes
- id
- afa697d2-5af1-4a37-b374-b5255888dea6 (old id 106435)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11874865&dopt=Abstract
- date added to LUP
- 2016-04-01 15:35:55
- date last changed
- 2022-02-27 07:46:50
@article{afa697d2-5af1-4a37-b374-b5255888dea6, abstract = {{This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing.}}, author = {{Du, Liping and Fukushima, Sachiko and Sallmyr, Annahita and Manthorpe, Rolf and Bredberg, Anders}}, issn = {{1071-412X}}, keywords = {{Epitopes; Epithelial Cells/cytology/*immunology; Enzyme-Linked Immunosorbent Assay; DNA Damage; Autoimmune Diseases/diagnosis/immunology; Apoptosis/*immunology; Antigen-Antibody Reactions; Antinuclear/*analysis/immunology; Cultured; Antibodies; Human; Hypertonic Solutions; Osmotic Pressure; Support; Non-U.S. Gov't; Tumor Cells}}, language = {{eng}}, number = {{2}}, pages = {{287--294}}, publisher = {{American Society for Microbiology}}, series = {{Clinical and Diagnostic Laboratory Immunology}}, title = {{Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity.}}, url = {{https://lup.lub.lu.se/search/files/4429262/623575.pdf}}, doi = {{10.1128/CDLI.9.2.287-294.2002}}, volume = {{9}}, year = {{2002}}, }