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Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity.

Du, Liping LU ; Fukushima, Sachiko; Sallmyr, Annahita LU ; Manthorpe, Rolf LU and Bredberg, Anders LU (2002) In Clinical and Diagnostic Laboratory Immunology 9(2). p.287-294
Abstract
This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and... (More)
This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Epitopes, Epithelial Cells/cytology/*immunology, Enzyme-Linked Immunosorbent Assay, DNA Damage, Autoimmune Diseases/diagnosis/immunology, Apoptosis/*immunology, Antigen-Antibody Reactions, Antinuclear/*analysis/immunology, Cultured, Antibodies, Human, Hypertonic Solutions, Osmotic Pressure, Support, Non-U.S. Gov't, Tumor Cells
in
Clinical and Diagnostic Laboratory Immunology
volume
9
issue
2
pages
287 - 294
publisher
American Society for Microbiology
external identifiers
  • wos:000174457600012
  • pmid:11874865
  • scopus:0036126110
ISSN
1071-412X
DOI
10.1128/CDLI.9.2.287-294.2002
language
English
LU publication?
yes
id
afa697d2-5af1-4a37-b374-b5255888dea6 (old id 106435)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11874865&dopt=Abstract
date added to LUP
2007-07-04 12:08:38
date last changed
2017-01-01 06:42:49
@article{afa697d2-5af1-4a37-b374-b5255888dea6,
  abstract     = {This study of stress-related antinuclear antibody (ANA) reactivity was undertaken with the objective of improving clinical ANA testing. ANA was determined by parallel enzyme-linked immunosorbent assays of crude nuclear protein antigen extracted from HEp-2 cells either grown under optimal conditions (providing nonstress ANA antigen) or exposed to stress (providing stress ANA antigen). The stress stimuli used were gamma radiation (causing DNA damage) and a hypertonic environment (causing apoptosis). Signs of stress-related ANA reactivity were seen among connective tissue disease (CTD) patients (including patients with systemic lupus erythematosus; mixed CTD; calcinosis, Reynaud's phenomenon, esophageal motility disorders, sclerodactyly, and telangiectasia; scleroderma; and Sjögren's syndrome): 11% showed stress-positive ANA (i.e., a significantly stronger ANA reactivity with the extract from stressed cells), whereas 21% showed a markedly weaker reaction with the stress antigen. In contrast, among ANA screening patient sera, with no diagnosis of CTD, the fraction showing stress-positive ANA was higher (7 to 8%, depending on the type of stress) than among those showing a lower reactivity with stress antigen (1.5 to 2.5%). Only one serum among 89 (1%) tested sera from healthy individuals showed a stress-related ANA reaction. This demonstration of stress-related ANA suggests a means to improve the performance of clinical ANA testing.},
  author       = {Du, Liping and Fukushima, Sachiko and Sallmyr, Annahita and Manthorpe, Rolf and Bredberg, Anders},
  issn         = {1071-412X},
  keyword      = {Epitopes,Epithelial Cells/cytology/*immunology,Enzyme-Linked Immunosorbent Assay,DNA Damage,Autoimmune Diseases/diagnosis/immunology,Apoptosis/*immunology,Antigen-Antibody Reactions,Antinuclear/*analysis/immunology,Cultured,Antibodies,Human,Hypertonic Solutions,Osmotic Pressure,Support,Non-U.S. Gov't,Tumor Cells},
  language     = {eng},
  number       = {2},
  pages        = {287--294},
  publisher    = {American Society for Microbiology},
  series       = {Clinical and Diagnostic Laboratory Immunology},
  title        = {Exposure of HEp-2 Cells to Stress Conditions Influences Antinuclear Antibody Reactivity.},
  url          = {http://dx.doi.org/10.1128/CDLI.9.2.287-294.2002},
  volume       = {9},
  year         = {2002},
}