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Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells.

Barg, Sebastian LU ; Olofsson, Charlotta LU ; Schriever-Abeln, Jenny; Wendt, Anna LU ; Gebre-Medhin, Samuel LU ; Renström, Erik LU and Rorsman, Patrik LU (2002) In Neuron 33(2). p.287-299
Abstract
Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion... (More)
Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion pore is negligible and only proceeds after complete fusion, and (3) sluggish peptidergic transmission reflects the time course of vesicle emptying. (Less)
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organization
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published
subject
keywords
Peptides/*metabolism, PC12 Cells, Neurosecretory Systems/cytology/*metabolism, Membrane Fusion/*physiology, Luminescent Proteins, Kinetics, Indicators and Reagents, Hydrogen-Ion Concentration, Exocytosis/*physiology, Fluorescence, Rats, Secretory Vesicles/*metabolism, Support, Non-U.S. Gov't, Electric Stimulation, Cell Line, Animal
in
Neuron
volume
33
issue
2
pages
287 - 299
publisher
Cell Press
external identifiers
  • wos:000173387700015
  • pmid:11804575
  • scopus:0037122871
ISSN
0896-6273
DOI
10.1016/S0896-6273(02)00563-9
language
English
LU publication?
yes
id
a6b3a7f9-3ba8-45cb-9600-ae4a2b497f1f (old id 106536)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11804575&dopt=Abstract
date added to LUP
2007-07-10 10:38:39
date last changed
2017-11-26 03:25:28
@article{a6b3a7f9-3ba8-45cb-9600-ae4a2b497f1f,
  abstract     = {Peptidergic neurotransmission is slow compared to that mediated by classical neurotransmitters. We have studied exocytotic membrane fusion and cargo release by simultaneous capacitance measurements and confocal imaging of single secretory vesicles in neuroendocrine cells. Depletion of the readily releasable pool (RRP) correlated with exocytosis of 10%-20% of the docked vesicles. Some remaining vesicles became releasable after recovery of RRP. Expansion of the fusion pore, seen as an increase in luminal pH, occurred after approximately 0.3 s, and peptide release was delayed by another 1-10 s. We conclude that (1) RRP refilling involves chemical modification of vesicles already in place, (2) the release of large neuropeptides via the fusion pore is negligible and only proceeds after complete fusion, and (3) sluggish peptidergic transmission reflects the time course of vesicle emptying.},
  author       = {Barg, Sebastian and Olofsson, Charlotta and Schriever-Abeln, Jenny and Wendt, Anna and Gebre-Medhin, Samuel and Renström, Erik and Rorsman, Patrik},
  issn         = {0896-6273},
  keyword      = {Peptides/*metabolism,PC12 Cells,Neurosecretory Systems/cytology/*metabolism,Membrane Fusion/*physiology,Luminescent Proteins,Kinetics,Indicators and Reagents,Hydrogen-Ion Concentration,Exocytosis/*physiology,Fluorescence,Rats,Secretory Vesicles/*metabolism,Support,Non-U.S. Gov't,Electric Stimulation,Cell Line,Animal},
  language     = {eng},
  number       = {2},
  pages        = {287--299},
  publisher    = {Cell Press},
  series       = {Neuron},
  title        = {Delay between fusion pore opening and peptide release from large dense-core vesicles in neuroendocrine cells.},
  url          = {http://dx.doi.org/10.1016/S0896-6273(02)00563-9},
  volume       = {33},
  year         = {2002},
}