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Selective infection of E. coli as a function of a specific molecular interaction.

Nilsson, Nina; Karlsson, Fredrik LU ; Rakonjac, Jasna and Borrebaeck, Carl LU (2002) In Journal of Molecular Recognition 15(1). p.27-32
Abstract
Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected,... (More)
Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Protein Engineering, Peptide Library, Escherichia coli Infections/*microbiology, Bacteriophages/metabolism/*physiology, Escherichia coli/metabolism/*physiology, Support, Non-U.S. Gov't, U.S. Gov't, Non-P.H.S.
in
Journal of Molecular Recognition
volume
15
issue
1
pages
27 - 32
publisher
John Wiley & Sons
external identifiers
  • wos:000174191600005
  • scopus:0036006148
ISSN
1099-1352
DOI
10.1002/jmr.557
language
English
LU publication?
yes
id
b7fae7de-7bd0-49e0-996e-44a98e795c6e (old id 106655)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11870919&dopt=Abstract
date added to LUP
2007-07-09 13:27:41
date last changed
2017-01-01 04:47:22
@article{b7fae7de-7bd0-49e0-996e-44a98e795c6e,
  abstract     = {Selective infection of phage is when the bacterial infection depends on the specific molecular interaction between an antigen and a phage-displayed protein sequence such as an antibody. Engineering of the normal infection into pathways, directed by a specific protein--protein interaction, has raised several mechanistic questions. Here, we address the type of display and the affinity between the interacting pairs. The deleted phage R408d3 was used for the first time in selective infection and was shown to exhibit a superior performance compared to the VCSM13 phage. Furthermore, the affinity between the interacting pairs also affected the selective infection process and a correlation between affinity and infection efficiency was detected, thus implying that selective infection is the method of choice for selection of rare high-affinity interactions in molecular libraries.},
  author       = {Nilsson, Nina and Karlsson, Fredrik and Rakonjac, Jasna and Borrebaeck, Carl},
  issn         = {1099-1352},
  keyword      = {Protein Engineering,Peptide Library,Escherichia coli Infections/*microbiology,Bacteriophages/metabolism/*physiology,Escherichia coli/metabolism/*physiology,Support,Non-U.S. Gov't,U.S. Gov't,Non-P.H.S.},
  language     = {eng},
  number       = {1},
  pages        = {27--32},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Molecular Recognition},
  title        = {Selective infection of E. coli as a function of a specific molecular interaction.},
  url          = {http://dx.doi.org/10.1002/jmr.557},
  volume       = {15},
  year         = {2002},
}