The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.
(2002) In European Journal of Immunology 32(3). p.911-920- Abstract
- The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose-... (More)
- The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106687
- author
- Leandersson, Karin LU ; Ivars, Fredrik LU and Sigvardsson, Mikael LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gene Expression Regulation : genetics, Hela Cells, Genes T-Cell Receptor alpha, Hamsters, Enhancer Elements (Genetics), Electrophoretic Mobility Shift Assay, DNA-Binding Proteins/biosynthesis : genetics : physiology, Binding Sites, Animal, Promoter Regions (Genetics), Protein Binding, Protein Precursors : genetics, Proto-Oncogene Proteins : genetics : physiology, RNA Messenger : genetics, Recombinant Fusion Proteins : genetics : metabolism, Regulatory Sequences Nucleic Acid, Trans-Activation (Genetics), Trans-Activators : genetics : physiology, Transfection, Transcription Factors : physiology, Helix-Loop-Helix Motifs, Human, Mesocricetus, Mice, Mice Inbred C57BL
- in
- European Journal of Immunology
- volume
- 32
- issue
- 3
- pages
- 911 - 920
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000174439000035
- pmid:11870636
- scopus:0036201826
- ISSN
- 1521-4141
- DOI
- 10.1002/1521-4141(200203)32:3<911::AID-IMMU911>3.0.CO;2-H
- language
- English
- LU publication?
- yes
- id
- 006d4971-fc7b-42ab-9869-f71705eee06a (old id 106687)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11870636&dopt=Abstract
- date added to LUP
- 2016-04-01 12:04:03
- date last changed
- 2022-10-26 06:59:14
@article{006d4971-fc7b-42ab-9869-f71705eee06a, abstract = {{The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development.}}, author = {{Leandersson, Karin and Ivars, Fredrik and Sigvardsson, Mikael}}, issn = {{1521-4141}}, keywords = {{Gene Expression Regulation : genetics; Hela Cells; Genes T-Cell Receptor alpha; Hamsters; Enhancer Elements (Genetics); Electrophoretic Mobility Shift Assay; DNA-Binding Proteins/biosynthesis : genetics : physiology; Binding Sites; Animal; Promoter Regions (Genetics); Protein Binding; Protein Precursors : genetics; Proto-Oncogene Proteins : genetics : physiology; RNA Messenger : genetics; Recombinant Fusion Proteins : genetics : metabolism; Regulatory Sequences Nucleic Acid; Trans-Activation (Genetics); Trans-Activators : genetics : physiology; Transfection; Transcription Factors : physiology; Helix-Loop-Helix Motifs; Human; Mesocricetus; Mice; Mice Inbred C57BL}}, language = {{eng}}, number = {{3}}, pages = {{911--920}}, publisher = {{John Wiley & Sons Inc.}}, series = {{European Journal of Immunology}}, title = {{The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.}}, url = {{http://dx.doi.org/10.1002/1521-4141(200203)32:3<911::AID-IMMU911>3.0.CO;2-H}}, doi = {{10.1002/1521-4141(200203)32:3<911::AID-IMMU911>3.0.CO;2-H}}, volume = {{32}}, year = {{2002}}, }