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The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.

Leandersson, Karin LU ; Ivars, Fredrik LU and Sigvardsson, Mikael LU (2002) In European Journal of Immunology 32(3). p.911-920
Abstract
The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose-... (More)
The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Gene Expression Regulation : genetics, Hela Cells, Genes T-Cell Receptor alpha, Hamsters, Enhancer Elements (Genetics), Electrophoretic Mobility Shift Assay, DNA-Binding Proteins/biosynthesis : genetics : physiology, Binding Sites, Animal, Promoter Regions (Genetics), Protein Binding, Protein Precursors : genetics, Proto-Oncogene Proteins : genetics : physiology, RNA Messenger : genetics, Recombinant Fusion Proteins : genetics : metabolism, Regulatory Sequences Nucleic Acid, Trans-Activation (Genetics), Trans-Activators : genetics : physiology, Transfection, Transcription Factors : physiology, Helix-Loop-Helix Motifs, Human, Mesocricetus, Mice, Mice Inbred C57BL
in
European Journal of Immunology
volume
32
issue
3
pages
911 - 920
publisher
John Wiley & Sons
external identifiers
  • wos:000174439000035
  • pmid:11870636
  • scopus:0036201826
ISSN
1521-4141
DOI
10.1002/1521-4141(200203)32:3<911::AID-IMMU911>3.0.CO;2-H
language
English
LU publication?
yes
id
006d4971-fc7b-42ab-9869-f71705eee06a (old id 106687)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11870636&dopt=Abstract
date added to LUP
2007-07-18 16:13:34
date last changed
2017-01-01 04:48:18
@article{006d4971-fc7b-42ab-9869-f71705eee06a,
  abstract     = {The pre-Talpha (pTalpha[?]) gene encodes a protein that forms the pre-TCR together with the newly expressed TCR beta chain during a defined stage of early T cell development. The restricted expression of this gene has been suggested to depend on a promoter and an enhancer element containing E boxes, which are potential binding sites for basic helix-loop-helix (bHLH) proteins. We here report that increased expression of E47 and E12 mRNA in CD4(--)CD8(--)TCR(--) thymocytes correlates with the initiation of pTalpha[?] transcription. Furthermore, electrophoretic mobility shift assays suggested that E47 binds directly to both the pTalpha[?] promoter and enhancer in vitro. Ectopic expression of E47 in non-lymphoid HeLa cells resultedin a dose- and template-dependent activation of these control elements. In addition to this, ectopic expression of E47 in combination with the ets-protein Pu.1 and[?]/[?]or the Pu.1-interacting protein(Pip-1), resulted in a synergistic effect on the activity of the pTalpha[?] control elements. Thus, we suggest that E47, Pu.1 and Pip-1 are directly involved in the regulation of pTalpha expression inearly T cell development.},
  author       = {Leandersson, Karin and Ivars, Fredrik and Sigvardsson, Mikael},
  issn         = {1521-4141},
  keyword      = {Gene Expression Regulation : genetics,Hela Cells,Genes T-Cell Receptor alpha,Hamsters,Enhancer Elements (Genetics),Electrophoretic Mobility Shift Assay,DNA-Binding Proteins/biosynthesis : genetics : physiology,Binding Sites,Animal,Promoter Regions (Genetics),Protein Binding,Protein Precursors : genetics,Proto-Oncogene Proteins : genetics : physiology,RNA Messenger : genetics,Recombinant Fusion Proteins : genetics : metabolism,Regulatory Sequences Nucleic Acid,Trans-Activation (Genetics),Trans-Activators : genetics : physiology,Transfection,Transcription Factors : physiology,Helix-Loop-Helix Motifs,Human,Mesocricetus,Mice,Mice Inbred C57BL},
  language     = {eng},
  number       = {3},
  pages        = {911--920},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {The pTalpha promoter and enhancer are direct targets for transactivation by E box-binding proteins.},
  url          = {http://dx.doi.org/10.1002/1521-4141(200203)32:3<911::AID-IMMU911>3.0.CO;2-H},
  volume       = {32},
  year         = {2002},
}