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Plasma proteins in a standardised skin mini-erosion (II): effects of extraction pressure.

Svedman, Christer; Yu, Bing; Ryan, Terence and Svensson, Henry LU (2002) In BMC Dermatology 2(1). p.4-4
Abstract
BACKGROUND: A standardised suction technique has been used to sample plasma proteins in dermal interstitial fluid (IF) serially for 5 to 6 days from a suction-induced skin mini-erosion. Increased protein concentrations ascribed to inflammation have been shown from day 1 onward. In this study, we assessed the effect of two different extraction pressures on IF sample composition. METHODS: Total protein concentration and the concentrations of insulin, prealbumin, albumin, transferrin, IgG and alpha-2-macroglobulin were assessed daily in healthy volunteers. Samples were extracted at 50 mmHg and 200 mmHg below the atmospheric. RESULTS: At 0 h after forming the erosion, mean total IF protein content (relative to plasma) was lower in the samples... (More)
BACKGROUND: A standardised suction technique has been used to sample plasma proteins in dermal interstitial fluid (IF) serially for 5 to 6 days from a suction-induced skin mini-erosion. Increased protein concentrations ascribed to inflammation have been shown from day 1 onward. In this study, we assessed the effect of two different extraction pressures on IF sample composition. METHODS: Total protein concentration and the concentrations of insulin, prealbumin, albumin, transferrin, IgG and alpha-2-macroglobulin were assessed daily in healthy volunteers. Samples were extracted at 50 mmHg and 200 mmHg below the atmospheric. RESULTS: At 0 h after forming the erosion, mean total IF protein content (relative to plasma) was lower in the samples extracted at -200 mmHg than at -50 mmHg (26 +/-13% (SD) vs 48 +/-9.8%; p < 0.05). There were no significant differences at 24, 48, 72 or 96 h. Of the individual proteins, expressed as area units (AU) for area under the curve (AUC) from 0&#ndash;96 h, albumin was lower in IF sampled at -200 mmHg (2.49 +/- 0.68 vs 3.08 +/- 0.36 AU; p < 0.05), as was transferrin (1.91 +/- 0.52 vs 2.40 +/- 0.42 AU; p < 0.05). Extraction volumes were significantly higher at -200 mmHg (AUC diff: 60%; p < 0.05). CONCLUSIONS: Samples of IF extracted at 0 h at -200 mmHg contained lower protein concentrations, indicating an increased water fraction and an intact sieve function of the vascular wall. The difference in protein concentration extracted at higher and lower pressure from 24 h onward was less pronounced. Lower pressure should be used to sample substances of greater molecular size. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
in
BMC Dermatology
volume
2
issue
1
pages
4 - 4
publisher
BioMed Central
external identifiers
  • scopus:0642379407
ISSN
1471-5945
language
English
LU publication?
yes
id
3821b48c-5dde-4e90-80c9-d77b2d7794ab (old id 106702)
alternative location
http://www.biomedcentral.com/content/pdf/1471-5945-2-4.pdf
date added to LUP
2007-07-30 14:01:49
date last changed
2017-01-01 06:55:10
@article{3821b48c-5dde-4e90-80c9-d77b2d7794ab,
  abstract     = {BACKGROUND: A standardised suction technique has been used to sample plasma proteins in dermal interstitial fluid (IF) serially for 5 to 6 days from a suction-induced skin mini-erosion. Increased protein concentrations ascribed to inflammation have been shown from day 1 onward. In this study, we assessed the effect of two different extraction pressures on IF sample composition. METHODS: Total protein concentration and the concentrations of insulin, prealbumin, albumin, transferrin, IgG and alpha-2-macroglobulin were assessed daily in healthy volunteers. Samples were extracted at 50 mmHg and 200 mmHg below the atmospheric. RESULTS: At 0 h after forming the erosion, mean total IF protein content (relative to plasma) was lower in the samples extracted at -200 mmHg than at -50 mmHg (26 +/-13% (SD) vs 48 +/-9.8%; p &lt; 0.05). There were no significant differences at 24, 48, 72 or 96 h. Of the individual proteins, expressed as area units (AU) for area under the curve (AUC) from 0&amp;#ndash;96 h, albumin was lower in IF sampled at -200 mmHg (2.49 +/- 0.68 vs 3.08 +/- 0.36 AU; p &lt; 0.05), as was transferrin (1.91 +/- 0.52 vs 2.40 +/- 0.42 AU; p &lt; 0.05). Extraction volumes were significantly higher at -200 mmHg (AUC diff: 60%; p &lt; 0.05). CONCLUSIONS: Samples of IF extracted at 0 h at -200 mmHg contained lower protein concentrations, indicating an increased water fraction and an intact sieve function of the vascular wall. The difference in protein concentration extracted at higher and lower pressure from 24 h onward was less pronounced. Lower pressure should be used to sample substances of greater molecular size.},
  author       = {Svedman, Christer and Yu, Bing and Ryan, Terence and Svensson, Henry},
  issn         = {1471-5945},
  language     = {eng},
  number       = {1},
  pages        = {4--4},
  publisher    = {BioMed Central},
  series       = {BMC Dermatology},
  title        = {Plasma proteins in a standardised skin mini-erosion (II): effects of extraction pressure.},
  volume       = {2},
  year         = {2002},
}