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Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.

Rosén, Monika LU and Edström, Jan-Erik (2002) In Chromosome Research 10(1). p.21-31
Abstract
Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for... (More)
Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
Animal, Chironomidae : genetics, Chromosomes : ultrastructure, DNA : genetics, Nucleic Acid Denaturation, Exodeoxyribonucleases, Oligodeoxyribonucleotides, Telomere : genetics, Support Non-U.S. Gov't, Base Sequence
in
Chromosome Research
volume
10
issue
1
pages
21 - 31
publisher
Springer
external identifiers
  • wos:000173846800003
  • scopus:0036164616
ISSN
1573-6849
DOI
10.1023/A:1014257808705
language
English
LU publication?
yes
id
e3c87797-6972-4c7e-ac36-a3073bcf80f6 (old id 106784)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11863067&dopt=Abstract
date added to LUP
2007-07-23 13:09:03
date last changed
2017-01-01 05:16:46
@article{e3c87797-6972-4c7e-ac36-a3073bcf80f6,
  abstract     = {Single-stranded overhangs of the G-rich strand belong to the conserved features of telomeres composed of short telomeric repeats. These structures are thought to be essential for the maintenance of proper telomeric structure and function and the mechanism of their generation is telomerase-independent. We have examined the presence of single-stranded overhangs in Chironomus tentans, a dipteran insect lacking canonical telomeres that uses 350-bp repeats to terminate its chromosomes. Using a non-denaturing in-gel hybridization technique, we found that C. tentans telomeres are unlikely to have single-stranded overhangs longer than 30 nt found in most other higher eukaryotes. These differences might reflect special capping mechanisms for telomeres terminated with long complex repeats.},
  author       = {Rosén, Monika and Edström, Jan-Erik},
  issn         = {1573-6849},
  keyword      = {Animal,Chironomidae : genetics,Chromosomes : ultrastructure,DNA : genetics,Nucleic Acid Denaturation,Exodeoxyribonucleases,Oligodeoxyribonucleotides,Telomere : genetics,Support Non-U.S. Gov't,Base Sequence},
  language     = {eng},
  number       = {1},
  pages        = {21--31},
  publisher    = {Springer},
  series       = {Chromosome Research},
  title        = {Chromosome ends in Chironomus tentans do not have long single-stranded overhangs characterizing canonical telomeres.},
  url          = {http://dx.doi.org/10.1023/A:1014257808705},
  volume       = {10},
  year         = {2002},
}