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Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.

Bal, Karol LU ; Larsson, Lennart LU ; Mielniczuk, Eugenia and Mielniczuk, Zbigniew (2002) In Journal of Microbiological Methods 48(2-3). p.267-270
Abstract
The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muramic Acids : chemistry, Trimethylsilyl Compounds : chemistry, Spectrum Analysis Mass, Peptidoglycan : analysis, Bacteria : chemistry
in
Journal of Microbiological Methods
volume
48
issue
2-3
pages
267 - 270
publisher
Elsevier
external identifiers
  • wos:000173483900016
  • pmid:11777574
  • scopus:0036147685
ISSN
1872-8359
DOI
10.1016/S0167-7012(01)00328-1
language
English
LU publication?
yes
id
4dd67462-04e3-4d4d-8bdf-38ac8102c4a5 (old id 106862)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11777574&dopt=Abstract
date added to LUP
2007-07-09 15:23:56
date last changed
2017-01-01 05:03:36
@article{4dd67462-04e3-4d4d-8bdf-38ac8102c4a5,
  abstract     = {The use of trimethylsilyl (TMS)-derivatisation for determining muramic acid in environmental and clinical samples by gas chromatography-mass spectrometry provides high detection sensitivity; however, questions have been raised as concerns the chemical structure of the entity giving the strong signal of m/z 185. In the present communication we present evidence that this entity results from the formation of a lactam structure of muramic acid upon derivatisation.},
  author       = {Bal, Karol and Larsson, Lennart and Mielniczuk, Eugenia and Mielniczuk, Zbigniew},
  issn         = {1872-8359},
  keyword      = {Muramic Acids : chemistry,Trimethylsilyl Compounds : chemistry,Spectrum Analysis Mass,Peptidoglycan : analysis,Bacteria : chemistry},
  language     = {eng},
  number       = {2-3},
  pages        = {267--270},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {Structure of muramic acid TMS derivative mass spectrum's base ion (m/z=185) used for quantification of bacterial peptidoglycan.},
  url          = {http://dx.doi.org/10.1016/S0167-7012(01)00328-1},
  volume       = {48},
  year         = {2002},
}