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Ly6C expression differentiates plasma cells from other B cell subsets in mice.

Wrammert, Jens LU ; Källberg, Eva LU ; Agace, William LU and Leanderson, Tomas LU (2002) In European Journal of Immunology 32(1). p.97-103
Abstract
Plasma cell differentiation is induced in vitro by lipopolysaccharide (LPS) stimulation but can be blocked by including anti-CD40 antibodies. Using subtractive cDNA hybridization we have identified the cell surface protein Ly6C as differentially expressed on B cells stimulated with LPS only. Ly6C has been shown to be expressed on certain T cell subsets and on subsets of macrophages and NK cells, but not on resting B cells. We show that Ly6C is up-regulated upon LPS stimulation of B cells in vitro and that this up-regulation is blocked by anti-CD40 or anti-Ig antibodies. Furthermore, ELISPOT analysis of cells sorted by magnetic-activated cell sorting show that Ly6C is expressed on ex vivo plasma cells from the spleen and bone marrow. Flow... (More)
Plasma cell differentiation is induced in vitro by lipopolysaccharide (LPS) stimulation but can be blocked by including anti-CD40 antibodies. Using subtractive cDNA hybridization we have identified the cell surface protein Ly6C as differentially expressed on B cells stimulated with LPS only. Ly6C has been shown to be expressed on certain T cell subsets and on subsets of macrophages and NK cells, but not on resting B cells. We show that Ly6C is up-regulated upon LPS stimulation of B cells in vitro and that this up-regulation is blocked by anti-CD40 or anti-Ig antibodies. Furthermore, ELISPOT analysis of cells sorted by magnetic-activated cell sorting show that Ly6C is expressed on ex vivo plasma cells from the spleen and bone marrow. Flow cytometric analysis showed that Ly6C is expressed on splenic plasma cells as well as on lamina propria plasma cells. Finally, Ly6C cross-linking positively up-regulated the amount of immunoglobulin produced by LPS-stimulated splenic B cells in vitro. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
B-Lymphocyte Subsets : cytology : drug effects : metabolism, Animal, Gene Expression, Intestine Small : cytology, Lipopolysaccharides : pharmacology, Membrane Glycoproteins : genetics : metabolism, Mice, Mice Inbred C57BL, Mitogens : pharmacology, Phosphatidylinositols : metabolism, Plasma Cells, Support Non-U.S. Gov't, Spleen : cytology, Biological Markers, Bone Marrow Cells : cytology, Cell Differentiation, Cell Lineage, Cells Cultured, Cross-Linking Reagents
in
European Journal of Immunology
volume
32
issue
1
pages
97 - 103
publisher
John Wiley & Sons
external identifiers
  • wos:000173527400011
  • pmid:11754008
  • scopus:0036149797
ISSN
1521-4141
DOI
10.1002/1521-4141(200201)32:1<97::AID-IMMU97>3.0.CO;2-Y
language
English
LU publication?
yes
id
4e4e3262-b478-4900-9820-cf6b8b57b263 (old id 106954)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11754008&dopt=Abstract
date added to LUP
2007-07-20 10:32:23
date last changed
2017-08-20 03:33:38
@article{4e4e3262-b478-4900-9820-cf6b8b57b263,
  abstract     = {Plasma cell differentiation is induced in vitro by lipopolysaccharide (LPS) stimulation but can be blocked by including anti-CD40 antibodies. Using subtractive cDNA hybridization we have identified the cell surface protein Ly6C as differentially expressed on B cells stimulated with LPS only. Ly6C has been shown to be expressed on certain T cell subsets and on subsets of macrophages and NK cells, but not on resting B cells. We show that Ly6C is up-regulated upon LPS stimulation of B cells in vitro and that this up-regulation is blocked by anti-CD40 or anti-Ig antibodies. Furthermore, ELISPOT analysis of cells sorted by magnetic-activated cell sorting show that Ly6C is expressed on ex vivo plasma cells from the spleen and bone marrow. Flow cytometric analysis showed that Ly6C is expressed on splenic plasma cells as well as on lamina propria plasma cells. Finally, Ly6C cross-linking positively up-regulated the amount of immunoglobulin produced by LPS-stimulated splenic B cells in vitro.},
  author       = {Wrammert, Jens and Källberg, Eva and Agace, William and Leanderson, Tomas},
  issn         = {1521-4141},
  keyword      = {B-Lymphocyte Subsets : cytology : drug effects : metabolism,Animal,Gene Expression,Intestine Small : cytology,Lipopolysaccharides : pharmacology,Membrane Glycoproteins : genetics : metabolism,Mice,Mice Inbred C57BL,Mitogens : pharmacology,Phosphatidylinositols : metabolism,Plasma Cells,Support  Non-U.S. Gov't,Spleen : cytology,Biological Markers,Bone Marrow Cells : cytology,Cell Differentiation,Cell Lineage,Cells Cultured,Cross-Linking Reagents},
  language     = {eng},
  number       = {1},
  pages        = {97--103},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Ly6C expression differentiates plasma cells from other B cell subsets in mice.},
  url          = {http://dx.doi.org/10.1002/1521-4141(200201)32:1<97::AID-IMMU97>3.0.CO;2-Y},
  volume       = {32},
  year         = {2002},
}