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Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for gamma-carboxyglutamic acid.

Brown, Mark A. LU ; Hambe, B; Furie, B; Furie, B C; Stenflo, Johan LU and Stenberg, Leisa LU (2002) In Toxicon 40(4). p.447-453
Abstract
gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and... (More)
gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and immunofluorescence assays. Amino acid analyses were also performed on most samples. A survey of 21 snake species from 12 genera detected gamma-carboxylated polypeptides only in venom of snakes from the elapid subfamily Acanthophiinae. Gla-containing polypeptides were also observed in cone snail venom but not in venom or toxic salivary secretions from several other organisms. The Gla-specific antibody used here provides a simple immunochemical means to detect gamma-carboxylated polypeptides in venom and may allow new species to be identified that utilize Gla in the biosynthesis of toxic polypeptides. (Less)
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keywords
Biological Assay : methods, Anura, Antibodies Monoclonal : diagnostic use, Blood Proteins : metabolism, Elapid Venoms : chemistry, Blotting Western, Factor Xa : analysis : metabolism, Fluorescent Antibody Technique, Mollusk Venoms : chemistry, Peptides : analysis : chemistry, Salivary Glands : chemistry, Support Non-U.S. Gov't, Snakes, Snails, Vitamin K : pharmacology, Animal, Amphibian Venoms : chemistry, 1-Carboxyglutamic Acid : analysis : immunology : pharmacology
in
Toxicon
volume
40
issue
4
pages
447 - 453
publisher
Elsevier
external identifiers
  • pmid:11738238
  • wos:000173462600008
  • scopus:0036135796
ISSN
0041-0101
DOI
10.1016/S0041-0101(01)00233-1
language
English
LU publication?
yes
id
5a27c91c-f650-4833-8480-e76c732f7754 (old id 107033)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11738238&dopt=Abstract
date added to LUP
2007-07-24 12:58:21
date last changed
2017-01-01 04:59:47
@article{5a27c91c-f650-4833-8480-e76c732f7754,
  abstract     = {gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and immunofluorescence assays. Amino acid analyses were also performed on most samples. A survey of 21 snake species from 12 genera detected gamma-carboxylated polypeptides only in venom of snakes from the elapid subfamily Acanthophiinae. Gla-containing polypeptides were also observed in cone snail venom but not in venom or toxic salivary secretions from several other organisms. The Gla-specific antibody used here provides a simple immunochemical means to detect gamma-carboxylated polypeptides in venom and may allow new species to be identified that utilize Gla in the biosynthesis of toxic polypeptides.},
  author       = {Brown, Mark A. and Hambe, B and Furie, B and Furie, B C and Stenflo, Johan and Stenberg, Leisa},
  issn         = {0041-0101},
  keyword      = {Biological Assay : methods,Anura,Antibodies Monoclonal : diagnostic use,Blood Proteins : metabolism,Elapid Venoms : chemistry,Blotting Western,Factor Xa : analysis : metabolism,Fluorescent Antibody Technique,Mollusk Venoms : chemistry,Peptides : analysis : chemistry,Salivary Glands : chemistry,Support Non-U.S. Gov't,Snakes,Snails,Vitamin K : pharmacology,Animal,Amphibian Venoms : chemistry,1-Carboxyglutamic Acid : analysis : immunology : pharmacology},
  language     = {eng},
  number       = {4},
  pages        = {447--453},
  publisher    = {Elsevier},
  series       = {Toxicon},
  title        = {Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for gamma-carboxyglutamic acid.},
  url          = {http://dx.doi.org/10.1016/S0041-0101(01)00233-1},
  volume       = {40},
  year         = {2002},
}