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n-CoDeR concept: unique types of antibodies for diagnostic use and therapy.

Carlsson, Roland LU and Söderlind, Eskil LU (2001) In Expert Review of Molecular Diagnostics 1(1). p.8-102
Abstract
The n-CoDeR recombinant antibody gene libraries are built on a single master framework, into which diverse in vivo-formed complementarity determining regions (CDRs) are allowed to recombine. These CDRs are sampled from in vivo-processed and proof-read gene sequences, thus ensuring an optimal level of correctly folded and functional molecules. By the modularized assembly process, up to six CDRs can be varied at the same time, providing a possibility for the creation of a hitherto undescribed genetic and functional variation. The n-CoDeR antibody gene libraries can be used to select highly specific, human antibody fragments with specificities to virtually any antigen, including carbohydrates and human self-proteins and with affinities down... (More)
The n-CoDeR recombinant antibody gene libraries are built on a single master framework, into which diverse in vivo-formed complementarity determining regions (CDRs) are allowed to recombine. These CDRs are sampled from in vivo-processed and proof-read gene sequences, thus ensuring an optimal level of correctly folded and functional molecules. By the modularized assembly process, up to six CDRs can be varied at the same time, providing a possibility for the creation of a hitherto undescribed genetic and functional variation. The n-CoDeR antibody gene libraries can be used to select highly specific, human antibody fragments with specificities to virtually any antigen, including carbohydrates and human self-proteins and with affinities down into the subnanomolar range. Furthermore, combining CDRs sampled from in vivo-processed sequences into a single framework result in molecules exhibiting a lower immunogenicity compared to normal human immunoglobulins, as determined by computer analyses. The distinguished features of the n-CoDeR libraries in the therapeutic and diagnostic areas are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal, Antibodies : diagnostic use, Antibodies : genetics, Antibodies : therapeutic use, Gene Library, Human, Immunoglobulin Fragments : genetics
in
Expert Review of Molecular Diagnostics
volume
1
issue
1
pages
8 - 102
publisher
Future Drugs Ltd
external identifiers
  • scopus:0035558749
ISSN
1744-8352
language
English
LU publication?
yes
id
f8d4aeac-dc2a-4600-9246-4a089acbd05b (old id 107135)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11901793&dopt=Abstract
date added to LUP
2007-06-27 11:36:21
date last changed
2018-05-29 10:03:48
@article{f8d4aeac-dc2a-4600-9246-4a089acbd05b,
  abstract     = {The n-CoDeR recombinant antibody gene libraries are built on a single master framework, into which diverse in vivo-formed complementarity determining regions (CDRs) are allowed to recombine. These CDRs are sampled from in vivo-processed and proof-read gene sequences, thus ensuring an optimal level of correctly folded and functional molecules. By the modularized assembly process, up to six CDRs can be varied at the same time, providing a possibility for the creation of a hitherto undescribed genetic and functional variation. The n-CoDeR antibody gene libraries can be used to select highly specific, human antibody fragments with specificities to virtually any antigen, including carbohydrates and human self-proteins and with affinities down into the subnanomolar range. Furthermore, combining CDRs sampled from in vivo-processed sequences into a single framework result in molecules exhibiting a lower immunogenicity compared to normal human immunoglobulins, as determined by computer analyses. The distinguished features of the n-CoDeR libraries in the therapeutic and diagnostic areas are discussed.},
  author       = {Carlsson, Roland and Söderlind, Eskil},
  issn         = {1744-8352},
  keyword      = {Animal,Antibodies : diagnostic use,Antibodies : genetics,Antibodies : therapeutic use,Gene Library,Human,Immunoglobulin Fragments : genetics},
  language     = {eng},
  number       = {1},
  pages        = {8--102},
  publisher    = {Future Drugs Ltd},
  series       = {Expert Review of Molecular Diagnostics},
  title        = {n-CoDeR concept: unique types of antibodies for diagnostic use and therapy.},
  volume       = {1},
  year         = {2001},
}