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Cyclin D1 amplification in chromosomal band 11q13 is associated with overrepresentation of 3q21-q29 in head and neck carcinomas.

Jin, Yuesheng LU ; Jin, Charlotte LU ; Wennerberg, Johan LU ; Höglund, Mattias LU and Mertens, Fredrik LU (2002) In International Journal of Cancer 98(3). p.475-479
Abstract
Eight cytogenetically characterized head and neck squamous cell carcinomas (HNSCCs) with CCND1 amplification in the form of a homogeneously staining region (hsr) in 11q13 were studied by COBRA FISH and FISH with specific probes to identify and characterize chromosomal segments added to the derivative chromosomes 11. In 4 of the tumors, it could be recognized that the material added was derived from the long arm of chromosome 3. The rearrangements were interpreted as der(11)hsr(11)(q13)t(3;11)(q21;q13) in 3 cases and as der(11)hsr(11)(q13)t(3;11)(q14;q13) in 1 case. In the other 4 cases, material from chromosomes 1, 16, or 19 was added to the derivative chromosomes 11. By further FISH analysis with 14 YAC clones spanning 3q13-q21 in the 4... (More)
Eight cytogenetically characterized head and neck squamous cell carcinomas (HNSCCs) with CCND1 amplification in the form of a homogeneously staining region (hsr) in 11q13 were studied by COBRA FISH and FISH with specific probes to identify and characterize chromosomal segments added to the derivative chromosomes 11. In 4 of the tumors, it could be recognized that the material added was derived from the long arm of chromosome 3. The rearrangements were interpreted as der(11)hsr(11)(q13)t(3;11)(q21;q13) in 3 cases and as der(11)hsr(11)(q13)t(3;11)(q14;q13) in 1 case. In the other 4 cases, material from chromosomes 1, 16, or 19 was added to the derivative chromosomes 11. By further FISH analysis with 14 YAC clones spanning 3q13-q21 in the 4 tumors with der(11)hsr(11)t(3;11), it could be shown that they had different breakpoints at the molecular level, excluding the possibility that a particular gene was rearranged by the translocations. More surprisingly, gain of the 3q21-q29 segment was found in all 8 tumors with hsr in 11q13 and loss of 3p was seen in 7 of the tumors. These findings strongly indicate a synergistic effect of CCND1 amplification, loss of distal 11q, 3q gain and 3p deletion in HNSCC development and also suggests a mechanistic link between intrachromosomal amplification at 11q13 and recombination with distal 3q. (Less)
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keywords
Head and Neck Neoplasms : metabolism, Male, Human, In Situ Hybridization, Fluorescence, Middle Age, Head and Neck Neoplasms : genetics, Gene Amplification, Female, Neoplasm : metabolism, DNA, Cyclin D1 : metabolism, Cyclin D1 : genetics, Pair 3 : genetics, Chromosomes, Pair 11 : genetics, Squamous Cell : metabolism, Carcinoma, Squamous Cell : genetics, Aged, Biopsy, Support, Non-U.S. Gov't
in
International Journal of Cancer
volume
98
issue
3
pages
475 - 479
publisher
John Wiley & Sons
external identifiers
  • pmid:11920603
  • wos:000174113800023
  • scopus:0037139397
ISSN
0020-7136
DOI
10.1002/ijc.10225
language
English
LU publication?
yes
id
b05654d7-56b9-421d-8c61-0194ba7e2d9a (old id 107261)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11920603&dopt=Abstract
date added to LUP
2007-07-07 08:56:49
date last changed
2017-01-01 04:45:21
@article{b05654d7-56b9-421d-8c61-0194ba7e2d9a,
  abstract     = {Eight cytogenetically characterized head and neck squamous cell carcinomas (HNSCCs) with CCND1 amplification in the form of a homogeneously staining region (hsr) in 11q13 were studied by COBRA FISH and FISH with specific probes to identify and characterize chromosomal segments added to the derivative chromosomes 11. In 4 of the tumors, it could be recognized that the material added was derived from the long arm of chromosome 3. The rearrangements were interpreted as der(11)hsr(11)(q13)t(3;11)(q21;q13) in 3 cases and as der(11)hsr(11)(q13)t(3;11)(q14;q13) in 1 case. In the other 4 cases, material from chromosomes 1, 16, or 19 was added to the derivative chromosomes 11. By further FISH analysis with 14 YAC clones spanning 3q13-q21 in the 4 tumors with der(11)hsr(11)t(3;11), it could be shown that they had different breakpoints at the molecular level, excluding the possibility that a particular gene was rearranged by the translocations. More surprisingly, gain of the 3q21-q29 segment was found in all 8 tumors with hsr in 11q13 and loss of 3p was seen in 7 of the tumors. These findings strongly indicate a synergistic effect of CCND1 amplification, loss of distal 11q, 3q gain and 3p deletion in HNSCC development and also suggests a mechanistic link between intrachromosomal amplification at 11q13 and recombination with distal 3q.},
  author       = {Jin, Yuesheng and Jin, Charlotte and Wennerberg, Johan and Höglund, Mattias and Mertens, Fredrik},
  issn         = {0020-7136},
  keyword      = {Head and Neck Neoplasms : metabolism,Male,Human,In Situ Hybridization,Fluorescence,Middle Age,Head and Neck Neoplasms : genetics,Gene Amplification,Female,Neoplasm : metabolism,DNA,Cyclin D1 : metabolism,Cyclin D1 : genetics,Pair 3 : genetics,Chromosomes,Pair 11 : genetics,Squamous Cell : metabolism,Carcinoma,Squamous Cell : genetics,Aged,Biopsy,Support,Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {475--479},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Cyclin D1 amplification in chromosomal band 11q13 is associated with overrepresentation of 3q21-q29 in head and neck carcinomas.},
  url          = {http://dx.doi.org/10.1002/ijc.10225},
  volume       = {98},
  year         = {2002},
}