Advanced

Antibody-mediated neutralization of cytomegalovirus: modulation of efficacy induced through the IgG constant region.

Furebring, Christina LU ; Speckner, Andrea; Mach, Michael; Sandlie, Inger; Norderhaug, Lars; Borrebaeck, Carl LU ; Turesson, Helene LU and Ohlin, Mats LU (2002) In Molecular Immunology 38(11). p.833-840
Abstract
Antibodies can neutralize the infectious properties of human cytomegalovirus (CMV). In vivo, the major neutralization determinants are located on glycoprotein B (gB). Recombinant human antibodies, that carry different constant regions (IgG1, IgG3 and the synthetic variant IgG3mA) against two of these epitopes were investigated for their ability to recruit the complement cascade for destruction of the virus. It was shown that all variants of an antibody against the antigenic domain (AD)-2 epitope displayed a similar neutralization activity despite the fact that improved C1q binding was observed for IgG3 and IgG3mA over the IgG1 variant. In contrast, an antibody against the AD-1 epitope carrying the normal IgG3 constant region, was less... (More)
Antibodies can neutralize the infectious properties of human cytomegalovirus (CMV). In vivo, the major neutralization determinants are located on glycoprotein B (gB). Recombinant human antibodies, that carry different constant regions (IgG1, IgG3 and the synthetic variant IgG3mA) against two of these epitopes were investigated for their ability to recruit the complement cascade for destruction of the virus. It was shown that all variants of an antibody against the antigenic domain (AD)-2 epitope displayed a similar neutralization activity despite the fact that improved C1q binding was observed for IgG3 and IgG3mA over the IgG1 variant. In contrast, an antibody against the AD-1 epitope carrying the normal IgG3 constant region, was less efficient than its IgG1 counterpart in neutralizing the virus in the absence of complement. However, it restored its activity in the presence of complement to the level of the naturally occurring IgG1 version. The same antibody was substantially more potent in neutralizing the virus in the presence of complement if it carried the IgG3mA constant region. This demonstrates the importance of the constant domain for the biological activity of AD-1 specific antibodies, a factor that should be taken into account when using antibody-based therapeutics or when inducing antibodies by vaccination. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neutralization Tests, Immunoglobulin G : immunology, Molecular Sequence Data, Immunoglobulin Constant Region : immunology, Human, Cytomegalovirus : immunology, Complement : physiology, Base Sequence, Viral : immunology, Antibodies, Recombinant Proteins : immunology, Support, Non-U.S. Gov't
in
Molecular Immunology
volume
38
issue
11
pages
833 - 840
publisher
Pergamon
external identifiers
  • PMID:11922941
  • WOS:000175293100004
  • Scopus:0036199486
ISSN
1872-9142
DOI
10.1016/S0161-5890(01)00119-5
language
English
LU publication?
yes
id
e118684c-0393-48b6-af7e-32823ec20de8 (old id 107287)
date added to LUP
2007-07-02 14:13:59
date last changed
2017-01-01 07:53:07
@article{e118684c-0393-48b6-af7e-32823ec20de8,
  abstract     = {Antibodies can neutralize the infectious properties of human cytomegalovirus (CMV). In vivo, the major neutralization determinants are located on glycoprotein B (gB). Recombinant human antibodies, that carry different constant regions (IgG1, IgG3 and the synthetic variant IgG3mA) against two of these epitopes were investigated for their ability to recruit the complement cascade for destruction of the virus. It was shown that all variants of an antibody against the antigenic domain (AD)-2 epitope displayed a similar neutralization activity despite the fact that improved C1q binding was observed for IgG3 and IgG3mA over the IgG1 variant. In contrast, an antibody against the AD-1 epitope carrying the normal IgG3 constant region, was less efficient than its IgG1 counterpart in neutralizing the virus in the absence of complement. However, it restored its activity in the presence of complement to the level of the naturally occurring IgG1 version. The same antibody was substantially more potent in neutralizing the virus in the presence of complement if it carried the IgG3mA constant region. This demonstrates the importance of the constant domain for the biological activity of AD-1 specific antibodies, a factor that should be taken into account when using antibody-based therapeutics or when inducing antibodies by vaccination.},
  author       = {Furebring, Christina and Speckner, Andrea and Mach, Michael and Sandlie, Inger and Norderhaug, Lars and Borrebaeck, Carl and Turesson, Helene and Ohlin, Mats},
  issn         = {1872-9142},
  keyword      = {Neutralization Tests,Immunoglobulin G : immunology,Molecular Sequence Data,Immunoglobulin Constant Region : immunology,Human,Cytomegalovirus : immunology,Complement : physiology,Base Sequence,Viral : immunology,Antibodies,Recombinant Proteins : immunology,Support,Non-U.S. Gov't},
  language     = {eng},
  number       = {11},
  pages        = {833--840},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Antibody-mediated neutralization of cytomegalovirus: modulation of efficacy induced through the IgG constant region.},
  url          = {http://dx.doi.org/10.1016/S0161-5890(01)00119-5},
  volume       = {38},
  year         = {2002},
}