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Scintillation proximity assay using molecularly imprinted microspheres.

Ye, Lei LU ; Surugiu, Ioana and Haupt, Karsten Olaf LU (2002) In Analytical Chemistry 74(5). p.959-964
Abstract
Molecularly imprinted microspheres were prepared as antibody binding mimics and used in scintillation proximity assay of a beta-adrenergic antagonist, (S)-propranolol. By using small polymer beads, we were able to place an organic scintillator and an "antenna" component in close proximity to the imprinted binding sites. When the radioactive template bound to the polymer, radiation energy was effectively transferred, via the antenna component, to the scintillator to generate a fluorescence signal. Using molecularly imprinted microspheres instead of antibodies, we have demonstrated competitive scintillation proximity assays for (S)-propranolol in both organic and aqueous solvents. The experimental results were further validated by normal... (More)
Molecularly imprinted microspheres were prepared as antibody binding mimics and used in scintillation proximity assay of a beta-adrenergic antagonist, (S)-propranolol. By using small polymer beads, we were able to place an organic scintillator and an "antenna" component in close proximity to the imprinted binding sites. When the radioactive template bound to the polymer, radiation energy was effectively transferred, via the antenna component, to the scintillator to generate a fluorescence signal. Using molecularly imprinted microspheres instead of antibodies, we have demonstrated competitive scintillation proximity assays for (S)-propranolol in both organic and aqueous solvents. The experimental results were further validated by normal ligand binding analysis, where liquid scintillation counting was used for quantification. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Isotope Labeling, Kinetics, Microspheres, Molecular Mimicry, Solvents, Propranolol : chemistry, Spectrometry, Fluorescence, Indicators and Reagents
in
Analytical Chemistry
volume
74
issue
5
pages
959 - 964
publisher
The American Chemical Society
external identifiers
  • pmid:11924998
  • wos:000174187900011
  • scopus:0036500308
ISSN
1520-6882
DOI
10.1021/ac015629e
language
English
LU publication?
yes
id
7f5115a4-a132-427a-900c-139b9f23c5a7 (old id 107314)
date added to LUP
2007-07-17 08:36:18
date last changed
2017-12-10 03:39:11
@article{7f5115a4-a132-427a-900c-139b9f23c5a7,
  abstract     = {Molecularly imprinted microspheres were prepared as antibody binding mimics and used in scintillation proximity assay of a beta-adrenergic antagonist, (S)-propranolol. By using small polymer beads, we were able to place an organic scintillator and an "antenna" component in close proximity to the imprinted binding sites. When the radioactive template bound to the polymer, radiation energy was effectively transferred, via the antenna component, to the scintillator to generate a fluorescence signal. Using molecularly imprinted microspheres instead of antibodies, we have demonstrated competitive scintillation proximity assays for (S)-propranolol in both organic and aqueous solvents. The experimental results were further validated by normal ligand binding analysis, where liquid scintillation counting was used for quantification.},
  author       = {Ye, Lei and Surugiu, Ioana and Haupt, Karsten Olaf},
  issn         = {1520-6882},
  keyword      = {Isotope Labeling,Kinetics,Microspheres,Molecular Mimicry,Solvents,Propranolol : chemistry,Spectrometry,Fluorescence,Indicators and Reagents},
  language     = {eng},
  number       = {5},
  pages        = {959--964},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Scintillation proximity assay using molecularly imprinted microspheres.},
  url          = {http://dx.doi.org/10.1021/ac015629e},
  volume       = {74},
  year         = {2002},
}