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Inhibitory effect on arterial injury-induced neointimal formation by adoptive B-cell transfer in Rag-1 knockout mice.

Dimayuga, Paul; Cercek, Bojan; Oguchi, Sumito; Nordin Fredrikson, Gunilla LU ; Yano, Juliana; Shah, Prediman K; Jovinge, Stefan LU and Nilsson, Jan LU (2002) In Arteriosclerosis, Thrombosis and Vascular Biology 22(4). p.9-644
Abstract
We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P<0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice... (More)
We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P<0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076+/-0.0039 [n=9] versus 0.020+/-0.0160 [n=8] mm(2), respectively; P<0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087+/-0.0037 [n=8] versus 0.0312+/-0.0174 [n=7] mm(2), respectively; P<0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury. (Less)
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keywords
Knockout, Mice, Male, Immunoglobulin M : blood, Immunoglobulin G : blood, Cellular, Immunity, Homeodomain Proteins : drug effects, Diet, Cholesterol : blood, Carotid Arteries : pathology, Carotid Arteries : immunology, B-Lymphocytes : transplantation, B-Lymphocytes : immunology, Adoptive Transfer, Animal, Models, Support, Non-U.S. Gov't, Tunica Intima : growth & development, Tunica Intima : immunology, Tunica Intima : injuries
in
Arteriosclerosis, Thrombosis and Vascular Biology
volume
22
issue
4
pages
9 - 644
publisher
American Heart Association
external identifiers
  • scopus:0036123835
ISSN
1524-4636
language
English
LU publication?
yes
id
9267346b-36c4-4e1f-816b-61cefa5b6272 (old id 107624)
alternative location
http://dx.doi.org/10.1161/01.ATV.0000012455.62765.BF
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11950704&dopt=Abstract
date added to LUP
2007-07-17 14:50:32
date last changed
2017-05-21 03:29:08
@article{9267346b-36c4-4e1f-816b-61cefa5b6272,
  abstract     = {We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P&lt;0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P&lt;0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076+/-0.0039 [n=9] versus 0.020+/-0.0160 [n=8] mm(2), respectively; P&lt;0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087+/-0.0037 [n=8] versus 0.0312+/-0.0174 [n=7] mm(2), respectively; P&lt;0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.},
  author       = {Dimayuga, Paul and Cercek, Bojan and Oguchi, Sumito and Nordin Fredrikson, Gunilla and Yano, Juliana and Shah, Prediman K and Jovinge, Stefan and Nilsson, Jan},
  issn         = {1524-4636},
  keyword      = {Knockout,Mice,Male,Immunoglobulin M : blood,Immunoglobulin G : blood,Cellular,Immunity,Homeodomain Proteins : drug effects,Diet,Cholesterol : blood,Carotid Arteries : pathology,Carotid Arteries : immunology,B-Lymphocytes : transplantation,B-Lymphocytes : immunology,Adoptive Transfer,Animal,Models,Support,Non-U.S. Gov't,Tunica Intima : growth & development,Tunica Intima : immunology,Tunica Intima : injuries},
  language     = {eng},
  number       = {4},
  pages        = {9--644},
  publisher    = {American Heart Association},
  series       = {Arteriosclerosis, Thrombosis and Vascular Biology},
  title        = {Inhibitory effect on arterial injury-induced neointimal formation by adoptive B-cell transfer in Rag-1 knockout mice.},
  volume       = {22},
  year         = {2002},
}