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Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.

Rosén, Monika LU ; Kamnert, I and Edström, J-E (2002) In Insect Molecular Biology 11(2). p.167-174
Abstract
We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.
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publication status
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subject
keywords
Animal, Northern, Non-U.S. Gov't, Support, Nucleic Acid, Repetitive Sequences, RNA, Genes, Insect, DNA Probes, DNA, Chironomidae : genetics, Blotting, Centrifugation, Isopycnic
in
Insect Molecular Biology
volume
11
issue
2
pages
167 - 174
publisher
Wiley-Blackwell
external identifiers
  • wos:000175251800007
  • scopus:0036011002
ISSN
1365-2583
DOI
10.1046/j.1365-2583.2002.00322.x
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Invertebral Developmental Biology, Udo Haecker's group (013212048)
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7b78c8bb-da64-456e-a29e-d120b2c704a7 (old id 107762)
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http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11966882&dopt=Abstract
date added to LUP
2016-04-01 16:33:15
date last changed
2020-12-29 04:35:30
@article{7b78c8bb-da64-456e-a29e-d120b2c704a7,
  abstract     = {We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.},
  author       = {Rosén, Monika and Kamnert, I and Edström, J-E},
  issn         = {1365-2583},
  language     = {eng},
  number       = {2},
  pages        = {167--174},
  publisher    = {Wiley-Blackwell},
  series       = {Insect Molecular Biology},
  title        = {Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.},
  url          = {http://dx.doi.org/10.1046/j.1365-2583.2002.00322.x},
  doi          = {10.1046/j.1365-2583.2002.00322.x},
  volume       = {11},
  year         = {2002},
}