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Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.

Rosén, Monika LU ; Kamnert, I and Edström, J-E (2002) In Insect Molecular Biology 11(2). p.167-174
Abstract
We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal, Northern, Non-U.S. Gov't, Support, Nucleic Acid, Repetitive Sequences, RNA, Genes, Insect, DNA Probes, DNA, Chironomidae : genetics, Blotting, Centrifugation, Isopycnic
in
Insect Molecular Biology
volume
11
issue
2
pages
167 - 174
publisher
Wiley-Blackwell
external identifiers
  • wos:000175251800007
  • scopus:0036011002
ISSN
1365-2583
DOI
10.1046/j.1365-2583.2002.00322.x
language
English
LU publication?
yes
id
7b78c8bb-da64-456e-a29e-d120b2c704a7 (old id 107762)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11966882&dopt=Abstract
date added to LUP
2007-07-23 13:01:27
date last changed
2017-01-01 07:08:25
@article{7b78c8bb-da64-456e-a29e-d120b2c704a7,
  abstract     = {We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.},
  author       = {Rosén, Monika and Kamnert, I and Edström, J-E},
  issn         = {1365-2583},
  keyword      = {Animal,Northern,Non-U.S. Gov't,Support,Nucleic Acid,Repetitive Sequences,RNA,Genes,Insect,DNA Probes,DNA,Chironomidae : genetics,Blotting,Centrifugation,Isopycnic},
  language     = {eng},
  number       = {2},
  pages        = {167--174},
  publisher    = {Wiley-Blackwell},
  series       = {Insect Molecular Biology},
  title        = {Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.},
  url          = {http://dx.doi.org/10.1046/j.1365-2583.2002.00322.x},
  volume       = {11},
  year         = {2002},
}