Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.
(2002) In Insect Molecular Biology 11(2). p.167-174- Abstract
- We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107762
- author
- Rosén, Monika LU ; Kamnert, I and Edström, J-E
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animal, Northern, Non-U.S. Gov't, Support, Nucleic Acid, Repetitive Sequences, RNA, Genes, Insect, DNA Probes, DNA, Chironomidae : genetics, Blotting, Centrifugation, Isopycnic
- in
- Insect Molecular Biology
- volume
- 11
- issue
- 2
- pages
- 167 - 174
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000175251800007
- scopus:0036011002
- ISSN
- 1365-2583
- DOI
- 10.1046/j.1365-2583.2002.00322.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Invertebral Developmental Biology, Udo Haecker's group (013212048)
- id
- 7b78c8bb-da64-456e-a29e-d120b2c704a7 (old id 107762)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11966882&dopt=Abstract
- date added to LUP
- 2016-04-01 16:33:15
- date last changed
- 2022-01-28 20:28:39
@article{7b78c8bb-da64-456e-a29e-d120b2c704a7, abstract = {{We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.}}, author = {{Rosén, Monika and Kamnert, I and Edström, J-E}}, issn = {{1365-2583}}, keywords = {{Animal; Northern; Non-U.S. Gov't; Support; Nucleic Acid; Repetitive Sequences; RNA; Genes; Insect; DNA Probes; DNA; Chironomidae : genetics; Blotting; Centrifugation; Isopycnic}}, language = {{eng}}, number = {{2}}, pages = {{167--174}}, publisher = {{Wiley-Blackwell}}, series = {{Insect Molecular Biology}}, title = {{Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.}}, url = {{http://dx.doi.org/10.1046/j.1365-2583.2002.00322.x}}, doi = {{10.1046/j.1365-2583.2002.00322.x}}, volume = {{11}}, year = {{2002}}, }