Ectopic localization of matrix metalloproteinase-9 in chronic cutaneous wounds.
(2002) In Human Pathology 33(3). p.355-364- Abstract
- It has been hypothesized that excessive activity of matrix metalloproteinases (MMPs), in particular the gelatinases MMP-9 and MMP-2, contributes to poor healing of chronic skin ulcers. We compared MMP-9 and MMP-2 in wound margin biopsies of standardized acute partial-thickness wounds in healthy volunteers (n = 6) and in venous leg ulcer patients (n = 12) with those of chronic wounds of different etiologies (n = 34) by a combination of specific analyses of activity and protein localization. We also studied MMP-14 by immunohistochemistry and in situ hybridization in parallel. Neither MMP-9 (P =.814) nor MMP-2 (P =.742) endogenous activities differed significantly between acute and chronic wound tissues. Acute wound healing was characterized... (More)
- It has been hypothesized that excessive activity of matrix metalloproteinases (MMPs), in particular the gelatinases MMP-9 and MMP-2, contributes to poor healing of chronic skin ulcers. We compared MMP-9 and MMP-2 in wound margin biopsies of standardized acute partial-thickness wounds in healthy volunteers (n = 6) and in venous leg ulcer patients (n = 12) with those of chronic wounds of different etiologies (n = 34) by a combination of specific analyses of activity and protein localization. We also studied MMP-14 by immunohistochemistry and in situ hybridization in parallel. Neither MMP-9 (P =.814) nor MMP-2 (P =.742) endogenous activities differed significantly between acute and chronic wound tissues. Acute wound healing was characterized by induction of MMP-9 in the advancing epithelium. In chronic wounds, prominent MMP-9 immunostaining was seen in neutrophils and macrophages in the ulcer bed, but virtually no MMP-9 was detected in wound edge keratinocytes. MMP-2 was increased and activated with acute wound age. MMP-2 was found abundantly in dermal fibroblasts and endothelial cells beneath, but not in new epithelium of acute and chronic wounds. MMP-14 mRNA or protein was detected solely in the stroma of both acute and chronic wounds. In conclusion, the overall activity of gelatinases MMP-9 and MMP-2 was not increased in chronic wounds compared to normally healing wound tissues. Chronic nonhealing wounds may not be caused by excessive gelatinase activity, but are distinguished from healing wounds by an unfavorable distribution and persistance of MMP-9. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107853
- author
- Mirastschijski, Ursula LU ; Impola, Ulla ; Jahkola, Tiina ; Karlsmark, Tonny ; AGren, Magnus S and Saarialho-Kere, Ulpu
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gelatinase A : genetics, Female, Chronic Disease, Cultured, Cells, 80 and over, Aged, Adult, Acute Disease, Leg Ulcer : pathology, Male, Metalloendopeptidases : genetics, Metalloendopeptidases : metabolism, Middle Age, Messenger : metabolism, RNA, Skin : enzymology, Skin : injuries, Skin : pathology, Support, Non-U.S. Gov't, Wounds and Injuries : pathology, Wound Healing : physiology, Wounds and Injuries : enzymology, Gelatinase A : metabolism, Gelatinase B : genetics, Gelatinase B : metabolism, Human, Immunohistochemistry, In Situ Hybridization, Leg Ulcer : enzymology
- in
- Human Pathology
- volume
- 33
- issue
- 3
- pages
- 355 - 364
- publisher
- Elsevier
- external identifiers
-
- wos:000175340300014
- pmid:11979378
- scopus:0036239786
- ISSN
- 1532-8392
- DOI
- 10.1053/hupa.2002.32221
- language
- English
- LU publication?
- yes
- id
- f08c4f3e-69df-49f5-a131-646b02aff3d2 (old id 107853)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11979378&dopt=Abstract
- date added to LUP
- 2016-04-01 12:09:53
- date last changed
- 2022-01-26 23:41:57
@article{f08c4f3e-69df-49f5-a131-646b02aff3d2,
abstract = {{It has been hypothesized that excessive activity of matrix metalloproteinases (MMPs), in particular the gelatinases MMP-9 and MMP-2, contributes to poor healing of chronic skin ulcers. We compared MMP-9 and MMP-2 in wound margin biopsies of standardized acute partial-thickness wounds in healthy volunteers (n = 6) and in venous leg ulcer patients (n = 12) with those of chronic wounds of different etiologies (n = 34) by a combination of specific analyses of activity and protein localization. We also studied MMP-14 by immunohistochemistry and in situ hybridization in parallel. Neither MMP-9 (P =.814) nor MMP-2 (P =.742) endogenous activities differed significantly between acute and chronic wound tissues. Acute wound healing was characterized by induction of MMP-9 in the advancing epithelium. In chronic wounds, prominent MMP-9 immunostaining was seen in neutrophils and macrophages in the ulcer bed, but virtually no MMP-9 was detected in wound edge keratinocytes. MMP-2 was increased and activated with acute wound age. MMP-2 was found abundantly in dermal fibroblasts and endothelial cells beneath, but not in new epithelium of acute and chronic wounds. MMP-14 mRNA or protein was detected solely in the stroma of both acute and chronic wounds. In conclusion, the overall activity of gelatinases MMP-9 and MMP-2 was not increased in chronic wounds compared to normally healing wound tissues. Chronic nonhealing wounds may not be caused by excessive gelatinase activity, but are distinguished from healing wounds by an unfavorable distribution and persistance of MMP-9.}},
author = {{Mirastschijski, Ursula and Impola, Ulla and Jahkola, Tiina and Karlsmark, Tonny and AGren, Magnus S and Saarialho-Kere, Ulpu}},
issn = {{1532-8392}},
keywords = {{Gelatinase A : genetics; Female; Chronic Disease; Cultured; Cells; 80 and over; Aged; Adult; Acute Disease; Leg Ulcer : pathology; Male; Metalloendopeptidases : genetics; Metalloendopeptidases : metabolism; Middle Age; Messenger : metabolism; RNA; Skin : enzymology; Skin : injuries; Skin : pathology; Support; Non-U.S. Gov't; Wounds and Injuries : pathology; Wound Healing : physiology; Wounds and Injuries : enzymology; Gelatinase A : metabolism; Gelatinase B : genetics; Gelatinase B : metabolism; Human; Immunohistochemistry; In Situ Hybridization; Leg Ulcer : enzymology}},
language = {{eng}},
number = {{3}},
pages = {{355--364}},
publisher = {{Elsevier}},
series = {{Human Pathology}},
title = {{Ectopic localization of matrix metalloproteinase-9 in chronic cutaneous wounds.}},
url = {{http://dx.doi.org/10.1053/hupa.2002.32221}},
doi = {{10.1053/hupa.2002.32221}},
volume = {{33}},
year = {{2002}},
}