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Phosphatidylethanol in rat organs after ethanol exposure.

Aradottir, Steina LU ; Lundqvist, Christofer and Alling, Christer LU (2002) In Alcoholism: Clinical and Experimental Research 26(4). p.514-518
Abstract
BACKGROUND: Phosphatidylethanol (PEth) is an abnormal phospholipid formed in mammalian cells that have been exposed to ethanol. It has been suggested that PEth mediates some of the damaging effects of ethanol on cells. This study was performed to investigate the level of PEth in organs of rats after in vivo alcohol exposure. METHODS: Three exposure models were studied: (1) acute, intraperitoneal injection of ethanol (n = 3 x 3); (2) chronic, forced ethanol drinking (n = 6); and (3) chronic, free choice of ethanol (n = 20). PEth was analyzed by high-performance liquid chromatography after lipid extraction of the organs. RESULTS: One acute injection gave detectable PEth levels in most organs analyzed, with maximal levels reached after 2 hr.... (More)
BACKGROUND: Phosphatidylethanol (PEth) is an abnormal phospholipid formed in mammalian cells that have been exposed to ethanol. It has been suggested that PEth mediates some of the damaging effects of ethanol on cells. This study was performed to investigate the level of PEth in organs of rats after in vivo alcohol exposure. METHODS: Three exposure models were studied: (1) acute, intraperitoneal injection of ethanol (n = 3 x 3); (2) chronic, forced ethanol drinking (n = 6); and (3) chronic, free choice of ethanol (n = 20). PEth was analyzed by high-performance liquid chromatography after lipid extraction of the organs. RESULTS: One acute injection gave detectable PEth levels in most organs analyzed, with maximal levels reached after 2 hr. The highest levels were reached in intestines, stomach, and lung. No PEth was detected in skeletal muscle, pancreas, or testis. The two exposure models for oral intake of ethanol also gave detectable PEth levels in most organs. The highest levels were reached in stomach, lung, and spleen. PEth was detected in muscle only in animals with heavy total alcohol intake. CONCLUSIONS: PEth is formed in most organs of rats exposed to ethanol acutely or chronically. Variations in PEth level and rates of PEth formation and PEth degradation are organ specific. (Less)
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keywords
Support, Male, Rats, Sprague-Dawley, Tissue Distribution : physiology, Tissue Distribution : drug effects, Non-U.S. Gov't, Oral, Administration, Alcohol Drinking : metabolism, Animal, Central Nervous System Depressants : administration & dosage, Central Nervous System Depressants : pharmacokinetics, Choice Behavior : drug effects, Comparative Study, Drug, Dose-Response Relationship, Ethanol : administration & dosage, Injections, Glycerophospholipids : metabolism, Intraperitoneal, Ethanol : pharmacokinetics
in
Alcoholism: Clinical and Experimental Research
volume
26
issue
4
pages
514 - 518
publisher
Wiley-Blackwell
external identifiers
  • wos:000175175300012
  • pmid:11981128
  • scopus:0036117989
ISSN
0145-6008
language
English
LU publication?
yes
id
f27861c6-eb52-4936-a960-4eb4e08d219c (old id 107881)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11981128&dopt=Abstract
date added to LUP
2007-07-04 15:53:41
date last changed
2017-04-16 03:38:03
@article{f27861c6-eb52-4936-a960-4eb4e08d219c,
  abstract     = {BACKGROUND: Phosphatidylethanol (PEth) is an abnormal phospholipid formed in mammalian cells that have been exposed to ethanol. It has been suggested that PEth mediates some of the damaging effects of ethanol on cells. This study was performed to investigate the level of PEth in organs of rats after in vivo alcohol exposure. METHODS: Three exposure models were studied: (1) acute, intraperitoneal injection of ethanol (n = 3 x 3); (2) chronic, forced ethanol drinking (n = 6); and (3) chronic, free choice of ethanol (n = 20). PEth was analyzed by high-performance liquid chromatography after lipid extraction of the organs. RESULTS: One acute injection gave detectable PEth levels in most organs analyzed, with maximal levels reached after 2 hr. The highest levels were reached in intestines, stomach, and lung. No PEth was detected in skeletal muscle, pancreas, or testis. The two exposure models for oral intake of ethanol also gave detectable PEth levels in most organs. The highest levels were reached in stomach, lung, and spleen. PEth was detected in muscle only in animals with heavy total alcohol intake. CONCLUSIONS: PEth is formed in most organs of rats exposed to ethanol acutely or chronically. Variations in PEth level and rates of PEth formation and PEth degradation are organ specific.},
  author       = {Aradottir, Steina and Lundqvist, Christofer and Alling, Christer},
  issn         = {0145-6008},
  keyword      = {Support,Male,Rats,Sprague-Dawley,Tissue Distribution : physiology,Tissue Distribution : drug effects,Non-U.S. Gov't,Oral,Administration,Alcohol Drinking : metabolism,Animal,Central Nervous System Depressants : administration & dosage,Central Nervous System Depressants : pharmacokinetics,Choice Behavior : drug effects,Comparative Study,Drug,Dose-Response Relationship,Ethanol : administration & dosage,Injections,Glycerophospholipids : metabolism,Intraperitoneal,Ethanol : pharmacokinetics},
  language     = {eng},
  number       = {4},
  pages        = {514--518},
  publisher    = {Wiley-Blackwell},
  series       = {Alcoholism: Clinical and Experimental Research},
  title        = {Phosphatidylethanol in rat organs after ethanol exposure.},
  volume       = {26},
  year         = {2002},
}