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The human V-preB promoter is a target for coordinated activation by early B cell factor and E47.

Gisler, Ramiro LU and Sigvardsson, Mikael LU (2002) In Journal of Immunology 168(10). p.5130-5138
Abstract
The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity... (More)
The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity of a reporter gene under control of the VpreB promoter in epithelioid HeLa cells, an effect augmented by coexpression of the basic-helix-loop helix transcription factor E47. The ability to interact directly with E47 was shared by the promoters controlling the human mb-1 and B29 genes. These data indicate that the human VpreB promoter is a direct target for activation by EBF and E47 and that functional collaboration between these proteins may be of great importance in human B cell development. (Less)
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@article{62687724-562e-48f2-96c8-9c274f70f788,
  abstract     = {The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity of a reporter gene under control of the VpreB promoter in epithelioid HeLa cells, an effect augmented by coexpression of the basic-helix-loop helix transcription factor E47. The ability to interact directly with E47 was shared by the promoters controlling the human mb-1 and B29 genes. These data indicate that the human VpreB promoter is a direct target for activation by EBF and E47 and that functional collaboration between these proteins may be of great importance in human B cell development.},
  author       = {Gisler, Ramiro and Sigvardsson, Mikael},
  issn         = {1550-6606},
  keyword      = {Trans-Activators : physiology,Non-U.S. Gov't,Support,Promoter Regions (Genetics) : immunology,Mice,Molecular Sequence Data,Membrane Glycoproteins : metabolism,Membrane Glycoproteins : genetics,Jurkat Cells,Immunoglobulin Variable Region : genetics,Human,Hematopoietic Stem Cells : immunology,Hematopoietic Stem Cells : metabolism,Hematopoietic Stem Cells : cytology,Hela Cells,B-Lymphocyte,Light Chain,Gene Rearrangement,Transformed,DNA-Binding Proteins : physiology,Animal,B-Lymphocytes : cytology,Transcription Factors : physiology,Cell Line,Cell Differentiation : immunology,Cell Differentiation : genetics,Base Sequence,B-Lymphocytes : metabolism,B-Lymphocytes : immunology},
  language     = {eng},
  number       = {10},
  pages        = {5130--5138},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {The human V-preB promoter is a target for coordinated activation by early B cell factor and E47.},
  volume       = {168},
  year         = {2002},
}