The human V-preB promoter is a target for coordinated activation by early B cell factor and E47.
(2002) In Journal of Immunology 168(10). p.5130-5138- Abstract
- The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity... (More)
- The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity of a reporter gene under control of the VpreB promoter in epithelioid HeLa cells, an effect augmented by coexpression of the basic-helix-loop helix transcription factor E47. The ability to interact directly with E47 was shared by the promoters controlling the human mb-1 and B29 genes. These data indicate that the human VpreB promoter is a direct target for activation by EBF and E47 and that functional collaboration between these proteins may be of great importance in human B cell development. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108016
- author
- Gisler, Ramiro LU and Sigvardsson, Mikael LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Trans-Activators : physiology, Non-U.S. Gov't, Support, Promoter Regions (Genetics) : immunology, Mice, Molecular Sequence Data, Membrane Glycoproteins : metabolism, Membrane Glycoproteins : genetics, Jurkat Cells, Immunoglobulin Variable Region : genetics, Human, Hematopoietic Stem Cells : immunology, Hematopoietic Stem Cells : metabolism, Hematopoietic Stem Cells : cytology, Hela Cells, B-Lymphocyte, Light Chain, Gene Rearrangement, Transformed, DNA-Binding Proteins : physiology, Animal, B-Lymphocytes : cytology, Transcription Factors : physiology, Cell Line, Cell Differentiation : immunology, Cell Differentiation : genetics, Base Sequence, B-Lymphocytes : metabolism, B-Lymphocytes : immunology
- in
- Journal of Immunology
- volume
- 168
- issue
- 10
- pages
- 5130 - 5138
- publisher
- American Association of Immunologists
- external identifiers
-
- wos:000175480100038
- scopus:0037093872
- ISSN
- 1550-6606
- language
- English
- LU publication?
- yes
- id
- 62687724-562e-48f2-96c8-9c274f70f788 (old id 108016)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11994467&dopt=Abstract
- date added to LUP
- 2016-04-01 15:26:09
- date last changed
- 2022-04-30 06:59:00
@article{62687724-562e-48f2-96c8-9c274f70f788,
abstract = {{The development of mature B lymphoid cells involves a highly orchestrated regulation of stage- and lineage-specific genes. In this study, we report an analysis of the human surrogate L chain VpreB promoter. The promoter has an overall homology of 56% to the mouse counterpart and displays a preB cell-restricted activity in transient transfections in cell lines. The promoter harbors three independent binding sites for early B cell factor (EBF) as defined by EMSA and supershift experiments. These sites were important for the full function of the promoter in a preB cell line, and chromatin immunoprecipitation experiments indicate that EBF interacts with the promoter in vivo. In addition to this, ectopic expression of EBF induces the activity of a reporter gene under control of the VpreB promoter in epithelioid HeLa cells, an effect augmented by coexpression of the basic-helix-loop helix transcription factor E47. The ability to interact directly with E47 was shared by the promoters controlling the human mb-1 and B29 genes. These data indicate that the human VpreB promoter is a direct target for activation by EBF and E47 and that functional collaboration between these proteins may be of great importance in human B cell development.}},
author = {{Gisler, Ramiro and Sigvardsson, Mikael}},
issn = {{1550-6606}},
keywords = {{Trans-Activators : physiology; Non-U.S. Gov't; Support; Promoter Regions (Genetics) : immunology; Mice; Molecular Sequence Data; Membrane Glycoproteins : metabolism; Membrane Glycoproteins : genetics; Jurkat Cells; Immunoglobulin Variable Region : genetics; Human; Hematopoietic Stem Cells : immunology; Hematopoietic Stem Cells : metabolism; Hematopoietic Stem Cells : cytology; Hela Cells; B-Lymphocyte; Light Chain; Gene Rearrangement; Transformed; DNA-Binding Proteins : physiology; Animal; B-Lymphocytes : cytology; Transcription Factors : physiology; Cell Line; Cell Differentiation : immunology; Cell Differentiation : genetics; Base Sequence; B-Lymphocytes : metabolism; B-Lymphocytes : immunology}},
language = {{eng}},
number = {{10}},
pages = {{5130--5138}},
publisher = {{American Association of Immunologists}},
series = {{Journal of Immunology}},
title = {{The human V-preB promoter is a target for coordinated activation by early B cell factor and E47.}},
url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11994467&dopt=Abstract}},
volume = {{168}},
year = {{2002}},
}