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Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148

Whiteford, James R ; Xian, Xiaojie LU ; Chaussade, Claire ; Vanhaesebroeck, Bart ; Nourshargh, Sussan and Couchman, John R (2011) In Molecular Biology of the Cell 22(19). p.24-3609
Abstract

Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential... (More)

Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential implication of the C2β isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for β1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Cell Adhesion, Cell Line, Cytoskeleton/genetics, Fibroblasts/cytology, Gene Expression Regulation, Humans, Inflammation/genetics, Integrin beta1/genetics, Jurkat Cells, Ligands, Lung/cytology, Mice, Neovascularization, Physiologic/genetics, Phosphatidylinositol 3-Kinase/metabolism, Protein Interaction Domains and Motifs/genetics, RNA, Small Interfering, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 3/genetics, Signal Transduction, Syndecan-2/genetics, src-Family Kinases/metabolism
in
Molecular Biology of the Cell
volume
22
issue
19
pages
24 - 3609
publisher
American Society for Cell Biology
external identifiers
  • scopus:80053364451
  • pmid:21813734
ISSN
1939-4586
DOI
10.1091/mbc.E11-02-0099
language
English
LU publication?
no
id
10803864-3db9-4fc5-bc36-57056741745e
date added to LUP
2021-04-22 16:20:32
date last changed
2024-06-29 12:25:20
@article{10803864-3db9-4fc5-bc36-57056741745e,
  abstract     = {{<p>Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential implication of the C2β isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for β1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.</p>}},
  author       = {{Whiteford, James R and Xian, Xiaojie and Chaussade, Claire and Vanhaesebroeck, Bart and Nourshargh, Sussan and Couchman, John R}},
  issn         = {{1939-4586}},
  keywords     = {{Animals; Cell Adhesion; Cell Line; Cytoskeleton/genetics; Fibroblasts/cytology; Gene Expression Regulation; Humans; Inflammation/genetics; Integrin beta1/genetics; Jurkat Cells; Ligands; Lung/cytology; Mice; Neovascularization, Physiologic/genetics; Phosphatidylinositol 3-Kinase/metabolism; Protein Interaction Domains and Motifs/genetics; RNA, Small Interfering; Rats; Receptor-Like Protein Tyrosine Phosphatases, Class 3/genetics; Signal Transduction; Syndecan-2/genetics; src-Family Kinases/metabolism}},
  language     = {{eng}},
  number       = {{19}},
  pages        = {{24--3609}},
  publisher    = {{American Society for Cell Biology}},
  series       = {{Molecular Biology of the Cell}},
  title        = {{Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148}},
  url          = {{http://dx.doi.org/10.1091/mbc.E11-02-0099}},
  doi          = {{10.1091/mbc.E11-02-0099}},
  volume       = {{22}},
  year         = {{2011}},
}