Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148
(2011) In Molecular Biology of the Cell 22(19). p.24-3609- Abstract
Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential... (More)
Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential implication of the C2β isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for β1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.
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- author
- Whiteford, James R ; Xian, Xiaojie LU ; Chaussade, Claire ; Vanhaesebroeck, Bart ; Nourshargh, Sussan and Couchman, John R
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Cell Adhesion, Cell Line, Cytoskeleton/genetics, Fibroblasts/cytology, Gene Expression Regulation, Humans, Inflammation/genetics, Integrin beta1/genetics, Jurkat Cells, Ligands, Lung/cytology, Mice, Neovascularization, Physiologic/genetics, Phosphatidylinositol 3-Kinase/metabolism, Protein Interaction Domains and Motifs/genetics, RNA, Small Interfering, Rats, Receptor-Like Protein Tyrosine Phosphatases, Class 3/genetics, Signal Transduction, Syndecan-2/genetics, src-Family Kinases/metabolism
- in
- Molecular Biology of the Cell
- volume
- 22
- issue
- 19
- pages
- 24 - 3609
- publisher
- American Society for Cell Biology
- external identifiers
-
- pmid:21813734
- scopus:80053364451
- ISSN
- 1939-4586
- DOI
- 10.1091/mbc.E11-02-0099
- language
- English
- LU publication?
- no
- id
- 10803864-3db9-4fc5-bc36-57056741745e
- date added to LUP
- 2021-04-22 16:20:32
- date last changed
- 2024-07-13 13:41:56
@article{10803864-3db9-4fc5-bc36-57056741745e, abstract = {{<p>Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the protein tyrosine phosphatase receptor CD148 is shown to be a key intermediary in cell adhesion to S2ED, with downstream β1 integrin-mediated adhesion and cytoskeletal organization. We show that S2ED is a novel ligand for CD148 and identify the region proximal to the transmembrane domain of syndecan-2 as the site of interaction with CD148. A mechanism for the transduction of the signal from CD148 to β1 integrins is elucidated requiring Src kinase and potential implication of the C2β isoform of phosphatidylinositol 3 kinase. Our data uncover a novel pathway for β1 integrin-mediated adhesion of importance in cellular processes such as angiogenesis and inflammation.</p>}}, author = {{Whiteford, James R and Xian, Xiaojie and Chaussade, Claire and Vanhaesebroeck, Bart and Nourshargh, Sussan and Couchman, John R}}, issn = {{1939-4586}}, keywords = {{Animals; Cell Adhesion; Cell Line; Cytoskeleton/genetics; Fibroblasts/cytology; Gene Expression Regulation; Humans; Inflammation/genetics; Integrin beta1/genetics; Jurkat Cells; Ligands; Lung/cytology; Mice; Neovascularization, Physiologic/genetics; Phosphatidylinositol 3-Kinase/metabolism; Protein Interaction Domains and Motifs/genetics; RNA, Small Interfering; Rats; Receptor-Like Protein Tyrosine Phosphatases, Class 3/genetics; Signal Transduction; Syndecan-2/genetics; src-Family Kinases/metabolism}}, language = {{eng}}, number = {{19}}, pages = {{24--3609}}, publisher = {{American Society for Cell Biology}}, series = {{Molecular Biology of the Cell}}, title = {{Syndecan-2 is a novel ligand for the protein tyrosine phosphatase receptor CD148}}, url = {{http://dx.doi.org/10.1091/mbc.E11-02-0099}}, doi = {{10.1091/mbc.E11-02-0099}}, volume = {{22}}, year = {{2011}}, }