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In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.

Dzhambazov, Balik LU ; Daskalova, Sashka; Monteva, Adriana and Popov, Nikola (2002) In Biological and Pharmaceutical Bulletin 25(4). p.499-504
Abstract
Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The... (More)
Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Biological and Pharmaceutical Bulletin
volume
25
issue
4
pages
499 - 504
publisher
The Pharmaceutical Society of Japan
external identifiers
  • wos:000174690200019
  • pmid:11995932
  • scopus:0036547619
ISSN
0918-6158
DOI
10.1248/bpb.25.499
language
English
LU publication?
yes
id
2fef4bc4-d25d-48bc-8c39-1cb505558daa (old id 108050)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11995932&dopt=Abstract
date added to LUP
2007-07-11 13:37:54
date last changed
2017-11-05 03:35:55
@article{2fef4bc4-d25d-48bc-8c39-1cb505558daa,
  abstract     = {Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible.},
  author       = {Dzhambazov, Balik and Daskalova, Sashka and Monteva, Adriana and Popov, Nikola},
  issn         = {0918-6158},
  language     = {eng},
  number       = {4},
  pages        = {499--504},
  publisher    = {The Pharmaceutical Society of Japan},
  series       = {Biological and Pharmaceutical Bulletin},
  title        = {In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.},
  url          = {http://dx.doi.org/10.1248/bpb.25.499},
  volume       = {25},
  year         = {2002},
}