In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.
(2002) In Biological and Pharmaceutical Bulletin 25(4). p.499-504- Abstract
- Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The... (More)
- Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108050
- author
- Dzhambazov, Balik LU ; Daskalova, Sashka ; Monteva, Adriana and Popov, Nikola
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biological and Pharmaceutical Bulletin
- volume
- 25
- issue
- 4
- pages
- 499 - 504
- publisher
- The Pharmaceutical Society of Japan
- external identifiers
-
- wos:000174690200019
- pmid:11995932
- scopus:0036547619
- ISSN
- 0918-6158
- DOI
- 10.1248/bpb.25.499
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 2fef4bc4-d25d-48bc-8c39-1cb505558daa (old id 108050)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11995932&dopt=Abstract
- date added to LUP
- 2016-04-01 11:57:47
- date last changed
- 2022-04-28 22:34:13
@article{2fef4bc4-d25d-48bc-8c39-1cb505558daa,
abstract = {{Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 microg/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 microg/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible.}},
author = {{Dzhambazov, Balik and Daskalova, Sashka and Monteva, Adriana and Popov, Nikola}},
issn = {{0918-6158}},
language = {{eng}},
number = {{4}},
pages = {{499--504}},
publisher = {{The Pharmaceutical Society of Japan}},
series = {{Biological and Pharmaceutical Bulletin}},
title = {{In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts.}},
url = {{http://dx.doi.org/10.1248/bpb.25.499}},
doi = {{10.1248/bpb.25.499}},
volume = {{25}},
year = {{2002}},
}