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Loss of size-selectivity at histamine-induced exudation of plasma proteins in atopic nasal airways.

Greiff, Lennart LU ; Andersson, Morgan LU ; Erjefält, Jonas LU ; Svensson, Christer LU and Persson, Carl LU (2002) In Clinical Physiology and Functional Imaging2002-01-01+01:00 22(1). p.28-31
Abstract
Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19.... (More)
Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19. However, at the histamine challenges the ratios were 25+/-3 and 22+/-2, respectively, which did not differ from that of circulating plasma (22+/-2). We conclude that there is minor and size-selective luminal entry of plasma proteins at baseline. However, at concentration-dependent exudative responses to histamine, plasma proteins enter the airway lumen without being sieved. These data indicate that inflammatory stimulus-induced extravasation, lamina propria distribution and paracellular epithelial passage of plasma occur with minimal size-selectivity. Inferentially, the full immunological capacity of plasma proteins may readily be made available at the surface of human intact airway mucosa. (Less)
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keywords
Dose-Response Relationship, Drug, Histamine : administration & dosage, Histamine : pharmacology, Human, Hypersensitivity : metabolism, Nasal Lavage Fluid : chemistry, Nasal Mucosa : drug effects, alpha-Macroglobulins : metabolism, Non-U.S. Gov't, Support, Serum Albumin : metabolism, Nasal Mucosa : metabolism, Permeability, Blood Proteins : metabolism, Adult
in
Clinical Physiology and Functional Imaging2002-01-01+01:00
volume
22
issue
1
pages
28 - 31
publisher
Wiley Online Library
external identifiers
  • wos:000174885800008
  • pmid:12003096
  • scopus:0036246738
ISSN
1475-0961
DOI
10.1046/j.1475-097X.2002.00390.x
language
English
LU publication?
yes
id
ad0c6e2f-2089-4ef0-bd99-bb5ba6b1f976 (old id 108117)
date added to LUP
2007-07-09 11:49:08
date last changed
2017-09-17 04:38:03
@article{ad0c6e2f-2089-4ef0-bd99-bb5ba6b1f976,
  abstract     = {Plasma proteins occur in the airway lumen in inflammatory airway diseases. This study tests the hypothesis that airway microvascular-epithelial exudation of plasma proteins, as induced by a non-injurious inflammatory mediator, is characterized by loss of size-selectivity. Using a nasal pool-device, the nasal mucosa of 10 allergic individuals, without current disease, was sequentially exposed to saline and histamine (40 and 400 microg ml(-1)). Nasal lavage fluid and blood-levels of albumin (69 kD) and alpha2-macroglobulin (720 kD) were determined. Histamine produced concentration-dependent exudation of albumin and alpha2-macroglobulin. The albumin/alpha2-macroglobulin concentration ratio of the saline lavage fluid (baseline) was 40+/-19. However, at the histamine challenges the ratios were 25+/-3 and 22+/-2, respectively, which did not differ from that of circulating plasma (22+/-2). We conclude that there is minor and size-selective luminal entry of plasma proteins at baseline. However, at concentration-dependent exudative responses to histamine, plasma proteins enter the airway lumen without being sieved. These data indicate that inflammatory stimulus-induced extravasation, lamina propria distribution and paracellular epithelial passage of plasma occur with minimal size-selectivity. Inferentially, the full immunological capacity of plasma proteins may readily be made available at the surface of human intact airway mucosa.},
  author       = {Greiff, Lennart and Andersson, Morgan and Erjefält, Jonas and Svensson, Christer and Persson, Carl},
  issn         = {1475-0961},
  keyword      = {Dose-Response Relationship,Drug,Histamine : administration & dosage,Histamine : pharmacology,Human,Hypersensitivity : metabolism,Nasal Lavage Fluid : chemistry,Nasal Mucosa : drug effects,alpha-Macroglobulins : metabolism,Non-U.S. Gov't,Support,Serum Albumin : metabolism,Nasal Mucosa : metabolism,Permeability,Blood Proteins : metabolism,Adult},
  language     = {eng},
  number       = {1},
  pages        = {28--31},
  publisher    = {Wiley Online Library},
  series       = {Clinical Physiology and Functional Imaging2002-01-01+01:00},
  title        = {Loss of size-selectivity at histamine-induced exudation of plasma proteins in atopic nasal airways.},
  url          = {http://dx.doi.org/10.1046/j.1475-097X.2002.00390.x},
  volume       = {22},
  year         = {2002},
}