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Widespread changes in neurotransmitter expression and number of enteric neurons and interstitial cells of Cajal in lethal spotted mice: an explanation for persisting dysmotility after operation for Hirschsprung's disease?

Sandgren, Katarina; Larsson, Lars Torsten LU and Ekblad, Eva LU (2002) In Digestive Diseases and Sciences 47(5). p.1049-1064
Abstract
Gastrointestinal motor dysfunction persists in a large number of children subjected to surgical treatment for Hirschsprung's disease, indicating abnormalities in the remaining intestine. The aim of the study was to detect possible alterations in frequency and topographic distribution of enteric neurons and interstitial cells of Cajal in an experimental model (the lethal spotted mouse displaying a short rectal aganglionosis) for Hirschsprung's disease. Specimens from the intestinal tract from homozygous (aganglionic) and heterozygous (healthy littermates) were examined using histochemistry, in situ hybridization, and immunohistochemistry. In ileum and colon, ie, regions proximal to the aganglionosis, changes in the expression of... (More)
Gastrointestinal motor dysfunction persists in a large number of children subjected to surgical treatment for Hirschsprung's disease, indicating abnormalities in the remaining intestine. The aim of the study was to detect possible alterations in frequency and topographic distribution of enteric neurons and interstitial cells of Cajal in an experimental model (the lethal spotted mouse displaying a short rectal aganglionosis) for Hirschsprung's disease. Specimens from the intestinal tract from homozygous (aganglionic) and heterozygous (healthy littermates) were examined using histochemistry, in situ hybridization, and immunohistochemistry. In ileum and colon, ie, regions proximal to the aganglionosis, changes in the expression of neuropeptides and neuronal nitric oxide synthase and in the number of enteric neurons and interstitial cells of Cajal could be detected in homozygous versus heterozygous mice. The described changes are suggested to contribute to the dysmotility remaining after surgical resection of the aganglionic segment in Hirschsprung's disease. (Less)
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subject
keywords
Gastrointestinal System : cytology, Enteric Nervous System : cytology, Colon : innervation, Cell Count, Animal, Ileum : innervation, Immunohistochemistry, In Situ Hybridization, Mice, Mutant Strains, Smooth : cytology, Muscle, Neuropeptides : analysis, Neurotransmitters : analysis, Nitric-Oxide Synthase : analysis, Peristalsis : physiology, Rectum : innervation, Hirschsprung Disease : physiopathology, Hirschsprung Disease : surgery
in
Digestive Diseases and Sciences
volume
47
issue
5
pages
1049 - 1064
publisher
Springer
external identifiers
  • pmid:12018900
  • wos:000175501500015
  • scopus:0036232238
ISSN
1573-2568
DOI
10.1023/A:1015085923245
language
English
LU publication?
yes
id
791f880e-2bc2-46a2-a0c1-c9bcebb26bf0 (old id 108312)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=12018900&dopt=Abstract
date added to LUP
2007-07-19 13:53:29
date last changed
2017-09-24 03:32:05
@article{791f880e-2bc2-46a2-a0c1-c9bcebb26bf0,
  abstract     = {Gastrointestinal motor dysfunction persists in a large number of children subjected to surgical treatment for Hirschsprung's disease, indicating abnormalities in the remaining intestine. The aim of the study was to detect possible alterations in frequency and topographic distribution of enteric neurons and interstitial cells of Cajal in an experimental model (the lethal spotted mouse displaying a short rectal aganglionosis) for Hirschsprung's disease. Specimens from the intestinal tract from homozygous (aganglionic) and heterozygous (healthy littermates) were examined using histochemistry, in situ hybridization, and immunohistochemistry. In ileum and colon, ie, regions proximal to the aganglionosis, changes in the expression of neuropeptides and neuronal nitric oxide synthase and in the number of enteric neurons and interstitial cells of Cajal could be detected in homozygous versus heterozygous mice. The described changes are suggested to contribute to the dysmotility remaining after surgical resection of the aganglionic segment in Hirschsprung's disease.},
  author       = {Sandgren, Katarina and Larsson, Lars Torsten and Ekblad, Eva},
  issn         = {1573-2568},
  keyword      = {Gastrointestinal System : cytology,Enteric Nervous System : cytology,Colon : innervation,Cell Count,Animal,Ileum : innervation,Immunohistochemistry,In Situ Hybridization,Mice,Mutant Strains,Smooth : cytology,Muscle,Neuropeptides : analysis,Neurotransmitters : analysis,Nitric-Oxide Synthase : analysis,Peristalsis : physiology,Rectum : innervation,Hirschsprung Disease : physiopathology,Hirschsprung Disease : surgery},
  language     = {eng},
  number       = {5},
  pages        = {1049--1064},
  publisher    = {Springer},
  series       = {Digestive Diseases and Sciences},
  title        = {Widespread changes in neurotransmitter expression and number of enteric neurons and interstitial cells of Cajal in lethal spotted mice: an explanation for persisting dysmotility after operation for Hirschsprung's disease?},
  url          = {http://dx.doi.org/10.1023/A:1015085923245},
  volume       = {47},
  year         = {2002},
}