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The role of poly(ethyleneimine) in stabilization against metal-catalyzed oxidation of proteins: a case study with lactate dehydrogenase.

Breccia, Javier D; Andersson, Maria LU and Hatti-Kaul, Rajni LU (2002) In Biochimica et Biophysica Acta. General Subjects 1570(3). p.165-173
Abstract
The protection provided by poly(ethyleneimine) (PEI) to muscle lactate dehydrogenase (LDH) in metal-catalyzed oxidation (MCO) systems (CuSO(4) or FeCl(2) combined with H(2)O(2)) was studied, and comparisons were made with the chelators EDTA and desferal, respectively. The analytical chelating capacity of PEI was estimated to be around 1 mol Cu(2+)/10 mol ethyleneimine for all molecular weights of the polymer. The effect of [PEI monomer]/[metal ion] molar ratio on the oxidatively induced aggregation of LDH exhibited a similar trend as that of the other chelators; aggregation was enhanced at lower ratios and subsequently decreased until it was undetectable with increasing ratio. In contrast, the LDH activity showed a monotonic increase with... (More)
The protection provided by poly(ethyleneimine) (PEI) to muscle lactate dehydrogenase (LDH) in metal-catalyzed oxidation (MCO) systems (CuSO(4) or FeCl(2) combined with H(2)O(2)) was studied, and comparisons were made with the chelators EDTA and desferal, respectively. The analytical chelating capacity of PEI was estimated to be around 1 mol Cu(2+)/10 mol ethyleneimine for all molecular weights of the polymer. The effect of [PEI monomer]/[metal ion] molar ratio on the oxidatively induced aggregation of LDH exhibited a similar trend as that of the other chelators; aggregation was enhanced at lower ratios and subsequently decreased until it was undetectable with increasing ratio. In contrast, the LDH activity showed a monotonic increase with increasing concentrations of the chelator. Total protection to the enzyme by PEI was provided at concentrations lower than that needed for full chelation of the copper ions, i.e. at [PEI monomer]/[Cu(2+)] ratio above 9 in case of PEI 2000, and above 7 for PEI 25000 and 2.6 x 10(6), respectively. The polymer also provided protection against oxidation in an iron-based MCO system. Hydroxyl radical formation during the MCO reaction was inhibited in the presence of PEI. The polymer of higher molecular weights also exhibited a stronger free radical scavenging effect. (Less)
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organization
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publication status
published
subject
keywords
Non-U.S. Gov't, Iron : metabolism, Lactate Dehydrogenase : metabolism, Hydroxyl Radical : metabolism, Hydrogen Peroxide : metabolism, Free Radicals : metabolism, Enzyme Stability : drug effects, Copper : metabolism, Animal, Catalysis, Muscles : enzymology, Oxidation-Reduction, Polyethyleneimine : pharmacology, Protein Conformation : drug effects, Support
in
Biochimica et Biophysica Acta. General Subjects
volume
1570
issue
3
pages
165 - 173
publisher
Elsevier
external identifiers
  • wos:000176018300004
  • pmid:12020806
  • scopus:0037089462
ISSN
0304-4165
DOI
10.1016/S0304-4165(02)00193-9
language
English
LU publication?
yes
id
50c38ab8-1cbb-40cc-b682-06b7c8d0984a (old id 108343)
date added to LUP
2007-07-02 08:58:03
date last changed
2017-10-01 04:54:11
@article{50c38ab8-1cbb-40cc-b682-06b7c8d0984a,
  abstract     = {The protection provided by poly(ethyleneimine) (PEI) to muscle lactate dehydrogenase (LDH) in metal-catalyzed oxidation (MCO) systems (CuSO(4) or FeCl(2) combined with H(2)O(2)) was studied, and comparisons were made with the chelators EDTA and desferal, respectively. The analytical chelating capacity of PEI was estimated to be around 1 mol Cu(2+)/10 mol ethyleneimine for all molecular weights of the polymer. The effect of [PEI monomer]/[metal ion] molar ratio on the oxidatively induced aggregation of LDH exhibited a similar trend as that of the other chelators; aggregation was enhanced at lower ratios and subsequently decreased until it was undetectable with increasing ratio. In contrast, the LDH activity showed a monotonic increase with increasing concentrations of the chelator. Total protection to the enzyme by PEI was provided at concentrations lower than that needed for full chelation of the copper ions, i.e. at [PEI monomer]/[Cu(2+)] ratio above 9 in case of PEI 2000, and above 7 for PEI 25000 and 2.6 x 10(6), respectively. The polymer also provided protection against oxidation in an iron-based MCO system. Hydroxyl radical formation during the MCO reaction was inhibited in the presence of PEI. The polymer of higher molecular weights also exhibited a stronger free radical scavenging effect.},
  author       = {Breccia, Javier D and Andersson, Maria and Hatti-Kaul, Rajni},
  issn         = {0304-4165},
  keyword      = {Non-U.S. Gov't,Iron : metabolism,Lactate Dehydrogenase : metabolism,Hydroxyl Radical : metabolism,Hydrogen Peroxide : metabolism,Free Radicals : metabolism,Enzyme Stability : drug effects,Copper : metabolism,Animal,Catalysis,Muscles : enzymology,Oxidation-Reduction,Polyethyleneimine : pharmacology,Protein Conformation : drug effects,Support},
  language     = {eng},
  number       = {3},
  pages        = {165--173},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta. General Subjects},
  title        = {The role of poly(ethyleneimine) in stabilization against metal-catalyzed oxidation of proteins: a case study with lactate dehydrogenase.},
  url          = {http://dx.doi.org/10.1016/S0304-4165(02)00193-9},
  volume       = {1570},
  year         = {2002},
}