Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.

Smith, Emma; Gisler, Ramiro; Sigvardsson, Mikael

Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.

Mark

Smith, Emma ^LU ; Gisler, Ramiro ^LU and Sigvardsson, Mikael ^LU (2002) In Journal of Immunology 169(1). p.261-270

Abstract: The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally... (More); The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene. (Less)

Please use this url to cite or link to this publication: https://lup.lub.lu.se/record/108944

author

Smith, Emma ^LU ; Gisler, Ramiro ^LU and Sigvardsson, Mikael ^LU

organization

publishing date

2002

type

Contribution to journal

publication status

published

subject

Immunology in the medical area

keywords

Transcription Factors : physiology, Trans-Activators : biosynthesis, Inbred BALB C, Mice, Cells, Nuclear Proteins : metabolism, Organ Specificity : genetics, Protein Binding : immunology, Organ Specificity : immunology, Trans-Activators : genetics, E-Box Elements : genetics, E-Box Elements : immunology, Exons : genetics, Exons : immunology, Hela Cells, Helix-Loop-Helix Motifs : genetics, Helix-Loop-Helix Motifs : immunology, Human, Molecular Sequence Data, Nuclear Proteins : physiology, Promoter Regions (Genetics) : immunology, Protein Binding : genetics, Support, Non-U.S. Gov't, Trans-Activators : chemistry, Trans-Activators : physiology, Nuclear Proteins : genetics, Inbred C57BL, DNA-Binding Proteins : physiology, DNA-Binding Proteins : genetics, DNA-Binding Proteins : chemistry, DNA-Binding Proteins : biosynthesis, Molecular, Cloning, Cultured, 5' Untranslated Regions : genetics, 5' Untranslated Regions : immunology, Amino Acid Sequence, Animal, B-Lymphocytes : immunology, B-Lymphocytes : metabolism, Base Composition, Base Sequence

in

Journal of Immunology

volume

169

issue

1

pages

261 - 270

publisher

American Association of Immunologists

external identifiers

wos:000176360400034
pmid:12077253
scopus:0036644320

ISSN

1550-6606

language

English

LU publication?

yes

id

b3108e58-0fe5-4947-a574-56bb2fcf015e (old id 108944)

alternative location

http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12077253&dopt=Abstract

date added to LUP

2016-04-01 16:38:53

date last changed

2022-08-22 21:51:25

@article{b3108e58-0fe5-4947-a574-56bb2fcf015e,
  abstract     = {{The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene.}},
  author       = {{Smith, Emma and Gisler, Ramiro and Sigvardsson, Mikael}},
  issn         = {{1550-6606}},
  keywords     = {{Transcription Factors : physiology; Trans-Activators : biosynthesis; Inbred BALB C; Mice; Cells; Nuclear Proteins : metabolism; Organ Specificity : genetics; Protein Binding : immunology; Organ Specificity : immunology; Trans-Activators : genetics; E-Box Elements : genetics; E-Box Elements : immunology; Exons : genetics; Exons : immunology; Hela Cells; Helix-Loop-Helix Motifs : genetics; Helix-Loop-Helix Motifs : immunology; Human; Molecular Sequence Data; Nuclear Proteins : physiology; Promoter Regions (Genetics) : immunology; Protein Binding : genetics; Support; Non-U.S. Gov't; Trans-Activators : chemistry; Trans-Activators : physiology; Nuclear Proteins : genetics; Inbred C57BL; DNA-Binding Proteins : physiology; DNA-Binding Proteins : genetics; DNA-Binding Proteins : chemistry; DNA-Binding Proteins : biosynthesis; Molecular; Cloning; Cultured; 5' Untranslated Regions : genetics; 5' Untranslated Regions : immunology; Amino Acid Sequence; Animal; B-Lymphocytes : immunology; B-Lymphocytes : metabolism; Base Composition; Base Sequence}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{261--270}},
  publisher    = {{American Association of Immunologists}},
  series       = {{Journal of Immunology}},
  title        = {{Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.}},
  url          = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12077253&dopt=Abstract}},
  volume       = {{169}},
  year         = {{2002}},
}

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Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.