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Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.

Smith, Emma LU ; Gisler, Ramiro LU and Sigvardsson, Mikael LU (2002) In Journal of Immunology 169(1). p.261-270
Abstract
The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally... (More)
The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene. (Less)
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keywords
Transcription Factors : physiology, Trans-Activators : biosynthesis, Inbred BALB C, Mice, Cells, Nuclear Proteins : metabolism, Organ Specificity : genetics, Protein Binding : immunology, Organ Specificity : immunology, Trans-Activators : genetics, E-Box Elements : genetics, E-Box Elements : immunology, Exons : genetics, Exons : immunology, Hela Cells, Helix-Loop-Helix Motifs : genetics, Helix-Loop-Helix Motifs : immunology, Human, Molecular Sequence Data, Nuclear Proteins : physiology, Promoter Regions (Genetics) : immunology, Protein Binding : genetics, Support, Non-U.S. Gov't, Trans-Activators : chemistry, Trans-Activators : physiology, Nuclear Proteins : genetics, Inbred C57BL, DNA-Binding Proteins : physiology, DNA-Binding Proteins : genetics, DNA-Binding Proteins : chemistry, DNA-Binding Proteins : biosynthesis, Molecular, Cloning, Cultured, 5' Untranslated Regions : genetics, 5' Untranslated Regions : immunology, Amino Acid Sequence, Animal, B-Lymphocytes : immunology, B-Lymphocytes : metabolism, Base Composition, Base Sequence
in
Journal of Immunology
volume
169
issue
1
pages
261 - 270
publisher
American Association of Immunologists
external identifiers
  • wos:000176360400034
  • pmid:12077253
  • scopus:0036644320
ISSN
1550-6606
language
English
LU publication?
yes
id
b3108e58-0fe5-4947-a574-56bb2fcf015e (old id 108944)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12077253&dopt=Abstract
date added to LUP
2007-07-24 09:41:02
date last changed
2017-01-01 07:11:00
@article{b3108e58-0fe5-4947-a574-56bb2fcf015e,
  abstract     = {The early B cell factor (EBF) is a transcription factor shown crucial for the development of B lymphocytes. The protein is expressed from the earliest stages of B cell development until the mature B cell stage, but the control elements responsible for the regulation of the gene are unknown. In this study, we report of the identification of a promoter region flanking the EBF gene. Several transcription start sites were identified by primer extension analysis in a region approximately 3.1 kb from the predicted ATG. Transient transfections revealed that this region was able to stimulate transcription of a reporter gene in B lymphoid and to a lesser extent, myeloid cells, but not in a pre-T cell line. The promoter was also able to functionally interact with E47, suggesting that the EBF gene may be a direct target for activation by E-proteins. In addition, functional binding of EBF to its own promoter was confirmed by EMSA and transfection assays indicating that the EBF protein may be involved in an autoregulatory loop. Finally, a tissue-restricted factor was able to bind an upstream regulatory region in B-lineage cells, further supporting the idea that the cloned promoter participates in the regulation of stage and lineage specific expression of the EBF gene.},
  author       = {Smith, Emma and Gisler, Ramiro and Sigvardsson, Mikael},
  issn         = {1550-6606},
  keyword      = {Transcription Factors : physiology,Trans-Activators : biosynthesis,Inbred BALB C,Mice,Cells,Nuclear Proteins : metabolism,Organ Specificity : genetics,Protein Binding : immunology,Organ Specificity : immunology,Trans-Activators : genetics,E-Box Elements : genetics,E-Box Elements : immunology,Exons : genetics,Exons : immunology,Hela Cells,Helix-Loop-Helix Motifs : genetics,Helix-Loop-Helix Motifs : immunology,Human,Molecular Sequence Data,Nuclear Proteins : physiology,Promoter Regions (Genetics) : immunology,Protein Binding : genetics,Support,Non-U.S. Gov't,Trans-Activators : chemistry,Trans-Activators : physiology,Nuclear Proteins : genetics,Inbred C57BL,DNA-Binding Proteins : physiology,DNA-Binding Proteins : genetics,DNA-Binding Proteins : chemistry,DNA-Binding Proteins : biosynthesis,Molecular,Cloning,Cultured,5' Untranslated Regions : genetics,5' Untranslated Regions : immunology,Amino Acid Sequence,Animal,B-Lymphocytes : immunology,B-Lymphocytes : metabolism,Base Composition,Base Sequence},
  language     = {eng},
  number       = {1},
  pages        = {261--270},
  publisher    = {American Association of Immunologists},
  series       = {Journal of Immunology},
  title        = {Cloning and characterization of a promoter flanking the early B cell factor (EBF) gene indicates roles for E-proteins and autoregulation in the control of EBF expression.},
  volume       = {169},
  year         = {2002},
}