Crystallization of a stringent response factor from Aquifex aeolicus.
(2002) In Acta Crystallographica. Section D: Biological Crystallography 58(Pt 7). p.1198-1200- Abstract
- The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/108949
- author
- Kristensen, Ole ; Laurberg, Martin LU and Gajhede, Michael
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- 58
- issue
- Pt 7
- pages
- 1198 - 1200
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:12077442
- wos:000176342000017
- scopus:0035997134
- ISSN
- 1399-0047
- DOI
- 10.1107/S0907444902008417
- language
- English
- LU publication?
- yes
- id
- ad25f86d-6c77-495e-aa4f-bf1c5a9d26e5 (old id 108949)
- date added to LUP
- 2016-04-01 16:57:07
- date last changed
- 2022-01-28 23:17:30
@article{ad25f86d-6c77-495e-aa4f-bf1c5a9d26e5, abstract = {{The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.}}, author = {{Kristensen, Ole and Laurberg, Martin and Gajhede, Michael}}, issn = {{1399-0047}}, language = {{eng}}, number = {{Pt 7}}, pages = {{1198--1200}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Acta Crystallographica. Section D: Biological Crystallography}}, title = {{Crystallization of a stringent response factor from Aquifex aeolicus.}}, url = {{http://dx.doi.org/10.1107/S0907444902008417}}, doi = {{10.1107/S0907444902008417}}, volume = {{58}}, year = {{2002}}, }