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Crystallization of a stringent response factor from Aquifex aeolicus.

Kristensen, Ole; Laurberg, Martin LU and Gajhede, Michael (2002) In Acta Crystallographica. Section D: Biological Crystallography 58(Pt 7). p.1198-1200
Abstract
The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Crystallographica. Section D: Biological Crystallography
volume
58
issue
Pt 7
pages
1198 - 1200
publisher
International Union of Crystallography
external identifiers
  • pmid:12077442
  • wos:000176342000017
  • scopus:0035997134
ISSN
1399-0047
DOI
10.1107/S0907444902008417
language
English
LU publication?
yes
id
ad25f86d-6c77-495e-aa4f-bf1c5a9d26e5 (old id 108949)
date added to LUP
2007-07-09 15:45:26
date last changed
2017-01-01 07:19:39
@article{ad25f86d-6c77-495e-aa4f-bf1c5a9d26e5,
  abstract     = {The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.},
  author       = {Kristensen, Ole and Laurberg, Martin and Gajhede, Michael},
  issn         = {1399-0047},
  language     = {eng},
  number       = {Pt 7},
  pages        = {1198--1200},
  publisher    = {International Union of Crystallography},
  series       = {Acta Crystallographica. Section D: Biological Crystallography},
  title        = {Crystallization of a stringent response factor from Aquifex aeolicus.},
  url          = {http://dx.doi.org/10.1107/S0907444902008417},
  volume       = {58},
  year         = {2002},
}