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Harmful azo colorants in leather. Determination based on their cleavage and extraction of corresponding carcinogenic aromatic amines using modern extraction techniques.

Sparr Eskilsson, Cecilia LU ; Davidsson, Richard LU and Mathiasson, Lennart LU (2002) In Journal of Chromatography A 955(2). p.215-227
Abstract
This study concerns the possibilities of using microwave-assisted extraction (MAE) or supercritical fluid extraction (SFE) for detection of harmful azo colorants in leather. After degreasing of the leather sample with SFE there follows a reductive cleavage of the azo colorants to their corresponding aromatic amines in the MAE or SFE equipment. The aromatic amines are subsequently extracted using either MAE or SFE and then finally determined by liquid chromatography with diode-array detection. The results have been compared with recoveries obtained using the German DIN method 53316. This standard method, based on conventional solvent extraction, is used in several European countries. Overall much better recoveries were obtained using MAE or... (More)
This study concerns the possibilities of using microwave-assisted extraction (MAE) or supercritical fluid extraction (SFE) for detection of harmful azo colorants in leather. After degreasing of the leather sample with SFE there follows a reductive cleavage of the azo colorants to their corresponding aromatic amines in the MAE or SFE equipment. The aromatic amines are subsequently extracted using either MAE or SFE and then finally determined by liquid chromatography with diode-array detection. The results have been compared with recoveries obtained using the German DIN method 53316. This standard method, based on conventional solvent extraction, is used in several European countries. Overall much better recoveries were obtained using MAE or SFE. With both MAE and SFE the amine recoveries of spiked leather samples were generally above 50%. The average recoveries were 62% for MAE and 60% for SFE (solvent collection) compared to 24% with the DIN method. For genuine leather samples the recoveries decreased, especially for benzidine. In this case the average values for MAE, SFE and DIN were 54, 38 and 19%, respectively. The quantification limits in leather samples using MAE or SFE were below 1 mg/kg for all amines investigated. The within-laboratory precision was generally better than 10%, varying somewhat with the analyte considered. With the proposed methodology, the amount of hazardous organic solvents used could be decreased and the sample throughput increased with at least a factor of two with less manual handling compared to the DIN method. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Azo dyes, aromatic, Amines
in
Journal of Chromatography A
volume
955
issue
2
pages
215 - 227
publisher
Elsevier
external identifiers
  • wos:000175793200006
  • pmid:12075925
  • scopus:0037052787
ISSN
0021-9673
DOI
10.1016/S0021-9673(02)00323-0
language
English
LU publication?
yes
id
c50d72f0-7350-448f-9243-a5d16f8d3e4e (old id 108954)
date added to LUP
2007-06-27 16:07:23
date last changed
2017-12-10 04:31:10
@article{c50d72f0-7350-448f-9243-a5d16f8d3e4e,
  abstract     = {This study concerns the possibilities of using microwave-assisted extraction (MAE) or supercritical fluid extraction (SFE) for detection of harmful azo colorants in leather. After degreasing of the leather sample with SFE there follows a reductive cleavage of the azo colorants to their corresponding aromatic amines in the MAE or SFE equipment. The aromatic amines are subsequently extracted using either MAE or SFE and then finally determined by liquid chromatography with diode-array detection. The results have been compared with recoveries obtained using the German DIN method 53316. This standard method, based on conventional solvent extraction, is used in several European countries. Overall much better recoveries were obtained using MAE or SFE. With both MAE and SFE the amine recoveries of spiked leather samples were generally above 50%. The average recoveries were 62% for MAE and 60% for SFE (solvent collection) compared to 24% with the DIN method. For genuine leather samples the recoveries decreased, especially for benzidine. In this case the average values for MAE, SFE and DIN were 54, 38 and 19%, respectively. The quantification limits in leather samples using MAE or SFE were below 1 mg/kg for all amines investigated. The within-laboratory precision was generally better than 10%, varying somewhat with the analyte considered. With the proposed methodology, the amount of hazardous organic solvents used could be decreased and the sample throughput increased with at least a factor of two with less manual handling compared to the DIN method.},
  author       = {Sparr Eskilsson, Cecilia and Davidsson, Richard and Mathiasson, Lennart},
  issn         = {0021-9673},
  keyword      = {Azo dyes,aromatic,Amines},
  language     = {eng},
  number       = {2},
  pages        = {215--227},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Harmful azo colorants in leather. Determination based on their cleavage and extraction of corresponding carcinogenic aromatic amines using modern extraction techniques.},
  url          = {http://dx.doi.org/10.1016/S0021-9673(02)00323-0},
  volume       = {955},
  year         = {2002},
}