Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells.
(2002) In Journal of Leukocyte Biology 72(1). p.147-153- Abstract
- Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells.... (More)
- Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109139
- author
- Bülow, Elinor LU ; Bengtsson, Niklas ; Calafat, Jero ; Gullberg, Urban LU and Olsson, Inge LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cytoplasmic Granules : metabolism, Cytoplasmic Granules : ultrastructure, Mast Cells : metabolism, Lysosomes : metabolism, Mast Cells : ultrastructure, Myeloid Cells : ultrastructure, Myeloid Cells : metabolism, Mice, Myeloid Progenitor Cells : metabolism, Neutrophils : chemistry, Protein Transport, Rats, Support, Non-U.S. Gov't, Transfection, Cell Line, Antimicrobial Cationic Peptides : metabolism, Antimicrobial Cationic Peptides : genetics, Antimicrobial Cationic Peptides : analysis, Animal
- in
- Journal of Leukocyte Biology
- volume
- 72
- issue
- 1
- pages
- 147 - 153
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:12101274
- wos:000176769100019
- scopus:0036658737
- ISSN
- 1938-3673
- language
- English
- LU publication?
- yes
- id
- 3822954b-4f01-40f4-904b-8f8d7e27c54a (old id 109139)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12101274&dopt=Abstract
- http://www.jleukbio.org/cgi/content/full/72/1/147
- date added to LUP
- 2016-04-01 12:37:15
- date last changed
- 2022-01-27 07:33:58
@article{3822954b-4f01-40f4-904b-8f8d7e27c54a, abstract = {{Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form.}}, author = {{Bülow, Elinor and Bengtsson, Niklas and Calafat, Jero and Gullberg, Urban and Olsson, Inge}}, issn = {{1938-3673}}, keywords = {{Cytoplasmic Granules : metabolism; Cytoplasmic Granules : ultrastructure; Mast Cells : metabolism; Lysosomes : metabolism; Mast Cells : ultrastructure; Myeloid Cells : ultrastructure; Myeloid Cells : metabolism; Mice; Myeloid Progenitor Cells : metabolism; Neutrophils : chemistry; Protein Transport; Rats; Support; Non-U.S. Gov't; Transfection; Cell Line; Antimicrobial Cationic Peptides : metabolism; Antimicrobial Cationic Peptides : genetics; Antimicrobial Cationic Peptides : analysis; Animal}}, language = {{eng}}, number = {{1}}, pages = {{147--153}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Leukocyte Biology}}, title = {{Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells.}}, url = {{http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12101274&dopt=Abstract}}, volume = {{72}}, year = {{2002}}, }