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Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells.

Bülow, Elinor LU ; Bengtsson, Niklas; Calafat, Jero; Gullberg, Urban LU and Olsson, Inge LU (2002) In Journal of Leukocyte Biology 72(1). p.147-153
Abstract
Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells.... (More)
Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form. (Less)
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keywords
Cytoplasmic Granules : metabolism, Cytoplasmic Granules : ultrastructure, Mast Cells : metabolism, Lysosomes : metabolism, Mast Cells : ultrastructure, Myeloid Cells : ultrastructure, Myeloid Cells : metabolism, Mice, Myeloid Progenitor Cells : metabolism, Neutrophils : chemistry, Protein Transport, Rats, Support, Non-U.S. Gov't, Transfection, Cell Line, Antimicrobial Cationic Peptides : metabolism, Antimicrobial Cationic Peptides : genetics, Antimicrobial Cationic Peptides : analysis, Animal
in
Journal of Leukocyte Biology
volume
72
issue
1
pages
147 - 153
publisher
Society for Leukocyte Biology
external identifiers
  • pmid:12101274
  • wos:000176769100019
  • scopus:0036658737
ISSN
1938-3673
language
English
LU publication?
yes
id
3822954b-4f01-40f4-904b-8f8d7e27c54a (old id 109139)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12101274&dopt=Abstract
http://www.jleukbio.org/cgi/content/full/72/1/147
date added to LUP
2007-07-09 12:21:19
date last changed
2017-01-01 05:18:23
@article{3822954b-4f01-40f4-904b-8f8d7e27c54a,
  abstract     = {Neutrophil granulocytes carry storage organelles, e.g., azurophil and specific granules. Poorly understood are the mechanisms for retrieval from constitutive secretion followed by sorting for storage. Therefore, we asked whether the specific granule protein human cathelicidin (hCAP-18) could be sorted for storage in other granules when the biosynthetic window is widened to allow this. We observed that hCAP-18 was targeted for storage in lysosome-related organelles when expressed constitutively in the rat basophilic leukemia and the mouse promyelocytic (MPRO) cell lines. In addition, premature release of the antibiotic C-terminal peptide LL-37 was observed. Retention of hCAP-18 was diminished by induction of differentiation of MPRO cells. In conclusion, a specific granule protein with native conformation may be sorted for storage in lysosome-related organelles of myeloid cells and converted prematurely to a supposedly biologically active form.},
  author       = {Bülow, Elinor and Bengtsson, Niklas and Calafat, Jero and Gullberg, Urban and Olsson, Inge},
  issn         = {1938-3673},
  keyword      = {Cytoplasmic Granules : metabolism,Cytoplasmic Granules : ultrastructure,Mast Cells : metabolism,Lysosomes : metabolism,Mast Cells : ultrastructure,Myeloid Cells : ultrastructure,Myeloid Cells : metabolism,Mice,Myeloid Progenitor Cells : metabolism,Neutrophils : chemistry,Protein Transport,Rats,Support,Non-U.S. Gov't,Transfection,Cell Line,Antimicrobial Cationic Peptides : metabolism,Antimicrobial Cationic Peptides : genetics,Antimicrobial Cationic Peptides : analysis,Animal},
  language     = {eng},
  number       = {1},
  pages        = {147--153},
  publisher    = {Society for Leukocyte Biology},
  series       = {Journal of Leukocyte Biology},
  title        = {Sorting of neutrophil-specific granule protein human cathelicidin, hCAP-18, when constitutively expressed in myeloid cells.},
  volume       = {72},
  year         = {2002},
}