Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A divalent antibody format is required for neutralization of human cytomegalovirus via antigenic domain 2 on glycoprotein B

Lantto, Johan LU ; Fletcher, Jean M and Ohlin, Mats LU orcid (2002) In Journal of General Virology 83(Pt 8). p.2001-2005
Abstract
Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can... (More)
Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can be mediated by dimeric scFv, while monomeric fragments cannot mediate neutralization of the virus, despite a slow dissociation from the intact glycoprotein. This finding is discussed in the context of possible mechanisms for antibody-mediated virus neutralization. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
83
issue
Pt 8
pages
2001 - 2005
publisher
Microbiology Society
external identifiers
  • pmid:12124464
  • wos:000176989900019
  • scopus:0036322438
ISSN
1465-2099
language
English
LU publication?
yes
id
dd6176c6-8f80-467a-89c6-467693d42e6e (old id 109429)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12124464&dopt=Abstract
date added to LUP
2016-04-01 15:25:53
date last changed
2022-03-30 01:15:09
@article{dd6176c6-8f80-467a-89c6-467693d42e6e,
  abstract     = {{Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can be mediated by dimeric scFv, while monomeric fragments cannot mediate neutralization of the virus, despite a slow dissociation from the intact glycoprotein. This finding is discussed in the context of possible mechanisms for antibody-mediated virus neutralization.}},
  author       = {{Lantto, Johan and Fletcher, Jean M and Ohlin, Mats}},
  issn         = {{1465-2099}},
  language     = {{eng}},
  number       = {{Pt 8}},
  pages        = {{2001--2005}},
  publisher    = {{Microbiology Society}},
  series       = {{Journal of General Virology}},
  title        = {{A divalent antibody format is required for neutralization of human cytomegalovirus via antigenic domain 2 on glycoprotein B}},
  url          = {{https://lup.lub.lu.se/search/files/4391149/623636.pdf}},
  volume       = {{83}},
  year         = {{2002}},
}