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Origins and projections of nerve fibres in rat pyloric sphincter.

Lindeström, Lille-Mor LU and Ekblad, Eva LU (2002) In Autonomic Neuroscience: Basic & Clinical2000-01-01+01:00 97(2). p.73-82
Abstract
Nerve fibres play an important role in the regulation of gastric emptying. The aims of this study were to clarify the distribution, projections and origin of neuronal type nitric oxide synthase (NOS)-, tyrosine hydroxylase (TH)-, vesicular acetylcholine transporter (VAchT)- and peptide-containing nerve fibres of the rat pyloric sphincter. Extrinsic and local denervations of the sphincter were performed in order to reveal the origin and projections of the various nerve fibre populations. Pylorus from control and denervated animals were processed for the immunocytochemical demonstration of cholecystokinin (CCK), enkephalin, gastrin-releasing peptide (GRP), somatostatin, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), pituitary... (More)
Nerve fibres play an important role in the regulation of gastric emptying. The aims of this study were to clarify the distribution, projections and origin of neuronal type nitric oxide synthase (NOS)-, tyrosine hydroxylase (TH)-, vesicular acetylcholine transporter (VAchT)- and peptide-containing nerve fibres of the rat pyloric sphincter. Extrinsic and local denervations of the sphincter were performed in order to reveal the origin and projections of the various nerve fibre populations. Pylorus from control and denervated animals were processed for the immunocytochemical demonstration of cholecystokinin (CCK), enkephalin, gastrin-releasing peptide (GRP), somatostatin, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), pituitary adenylate cyclase-activating peptide (PACAP), substance P (SP), vasoactive intestinal peptide (VIP), galanin, NOS, VAchT and TH. VAchT, TH, nNOS, and all of the peptides investigated were found in nerve fibres innervating the pyloric sphincter, and coexistence of several putative neurotransmitters were revealed. Extrinsic denervation caused a total loss of NPY/TH-, SP/CGRP- and SP/CGRP/VIP/NOS/PACAP-containing nerve fibres. Local denervation immediately proximal to the sphincter markedly reduced the numbers of VIP/NOS/galanin- and VIP/NOS/galanin/PACAP +/- NPY-containing fibres within the sphincter suggesting an origin of these fibres in myenteric ganglia in the antral region; denervation at the level of the oxyntic-pyloric border had no effect. Local denervation immediately distal to the sphincter caused a marked decrease in VAchT-, SP/enkephalin-, enkephalin-, somatostatin-, CCK- and GRP-containing fibres within the sphincter suggesting that these emanate from the duodenum. The latter procedure also reduced the number of SP/CGRP-containing fibres of extrinsic origin within the pyloric sphincter. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Neuropeptides, Nitric oxide synthase, Tyrosine hydroxylase, Vesicular acetylcholine transporter, Enteric nervous system, Pylorus
in
Autonomic Neuroscience: Basic & Clinical2000-01-01+01:00
volume
97
issue
2
pages
73 - 82
publisher
Elsevier
external identifiers
  • pmid:12132647
  • wos:000177002900001
  • scopus:0037205090
ISSN
1872-7484
DOI
10.1016/S1566-0702(02)00056-5
language
English
LU publication?
yes
id
bb038653-82ee-4af9-bec3-3e6a7411b9f5 (old id 109529)
date added to LUP
2007-07-16 16:03:34
date last changed
2017-01-01 05:13:51
@article{bb038653-82ee-4af9-bec3-3e6a7411b9f5,
  abstract     = {Nerve fibres play an important role in the regulation of gastric emptying. The aims of this study were to clarify the distribution, projections and origin of neuronal type nitric oxide synthase (NOS)-, tyrosine hydroxylase (TH)-, vesicular acetylcholine transporter (VAchT)- and peptide-containing nerve fibres of the rat pyloric sphincter. Extrinsic and local denervations of the sphincter were performed in order to reveal the origin and projections of the various nerve fibre populations. Pylorus from control and denervated animals were processed for the immunocytochemical demonstration of cholecystokinin (CCK), enkephalin, gastrin-releasing peptide (GRP), somatostatin, calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), pituitary adenylate cyclase-activating peptide (PACAP), substance P (SP), vasoactive intestinal peptide (VIP), galanin, NOS, VAchT and TH. VAchT, TH, nNOS, and all of the peptides investigated were found in nerve fibres innervating the pyloric sphincter, and coexistence of several putative neurotransmitters were revealed. Extrinsic denervation caused a total loss of NPY/TH-, SP/CGRP- and SP/CGRP/VIP/NOS/PACAP-containing nerve fibres. Local denervation immediately proximal to the sphincter markedly reduced the numbers of VIP/NOS/galanin- and VIP/NOS/galanin/PACAP +/- NPY-containing fibres within the sphincter suggesting an origin of these fibres in myenteric ganglia in the antral region; denervation at the level of the oxyntic-pyloric border had no effect. Local denervation immediately distal to the sphincter caused a marked decrease in VAchT-, SP/enkephalin-, enkephalin-, somatostatin-, CCK- and GRP-containing fibres within the sphincter suggesting that these emanate from the duodenum. The latter procedure also reduced the number of SP/CGRP-containing fibres of extrinsic origin within the pyloric sphincter.},
  author       = {Lindeström, Lille-Mor and Ekblad, Eva},
  issn         = {1872-7484},
  keyword      = {Neuropeptides,Nitric oxide synthase,Tyrosine hydroxylase,Vesicular acetylcholine transporter,Enteric nervous system,Pylorus},
  language     = {eng},
  number       = {2},
  pages        = {73--82},
  publisher    = {Elsevier},
  series       = {Autonomic Neuroscience: Basic & Clinical2000-01-01+01:00},
  title        = {Origins and projections of nerve fibres in rat pyloric sphincter.},
  url          = {http://dx.doi.org/10.1016/S1566-0702(02)00056-5},
  volume       = {97},
  year         = {2002},
}