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Transplantation of full-thickness retina in the normal porcine eye: surgical and morphologic aspects.

Ghosh, Fredrik LU and Arnér, Karin LU (2002) In Retina 22(4). p.478-486
Abstract
PURPOSE: To report a surgical technique for transplantation of full-thickness neuroretinal sheets into the subretinal space of a large animal with a vascularized retina and to establish the light microscopic morphology of such specimens. METHODS: Twelve normal pigs underwent transplantation of a neuroretinal sheet from a neonatal donor into the subretinal space by means of a vitrectomy-based technique. After a survival of 33 to 72 days, eye specimens were studied with a light microscope. RESULTS: In most eyes, the transplants displayed a laminated morphology, with photoreceptor outer segments facing the host retinal pigment epithelium. These grafts had normal outer retinal layers, while the inner layers were less developed. The host retina... (More)
PURPOSE: To report a surgical technique for transplantation of full-thickness neuroretinal sheets into the subretinal space of a large animal with a vascularized retina and to establish the light microscopic morphology of such specimens. METHODS: Twelve normal pigs underwent transplantation of a neuroretinal sheet from a neonatal donor into the subretinal space by means of a vitrectomy-based technique. After a survival of 33 to 72 days, eye specimens were studied with a light microscope. RESULTS: In most eyes, the transplants displayed a laminated morphology, with photoreceptor outer segments facing the host retinal pigment epithelium. These grafts had normal outer retinal layers, while the inner layers were less developed. The host retina straddling the graft showed evidence of photoreceptor degeneration, but the inner layers were well preserved. CONCLUSION: Full-thickness neuroretinal sheets can be transplanted to the subretinal space of a large animal eye with a vascularized retina. The grafts survive well and display mostly photoreceptors, which in combination with the well-preserved host inner retina may be of importance in attempts at reconstructing the retina in photoreceptor degenerative disease. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Retina
volume
22
issue
4
pages
478 - 486
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000177437200013
  • pmid:12172116
  • scopus:0036670704
ISSN
0275-004X
language
English
LU publication?
yes
id
a95a7349-2720-4966-b966-7bea0014a1c8 (old id 109867)
alternative location
http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12172116&dopt=Abstract
date added to LUP
2007-07-09 09:10:24
date last changed
2017-08-27 05:32:04
@article{a95a7349-2720-4966-b966-7bea0014a1c8,
  abstract     = {PURPOSE: To report a surgical technique for transplantation of full-thickness neuroretinal sheets into the subretinal space of a large animal with a vascularized retina and to establish the light microscopic morphology of such specimens. METHODS: Twelve normal pigs underwent transplantation of a neuroretinal sheet from a neonatal donor into the subretinal space by means of a vitrectomy-based technique. After a survival of 33 to 72 days, eye specimens were studied with a light microscope. RESULTS: In most eyes, the transplants displayed a laminated morphology, with photoreceptor outer segments facing the host retinal pigment epithelium. These grafts had normal outer retinal layers, while the inner layers were less developed. The host retina straddling the graft showed evidence of photoreceptor degeneration, but the inner layers were well preserved. CONCLUSION: Full-thickness neuroretinal sheets can be transplanted to the subretinal space of a large animal eye with a vascularized retina. The grafts survive well and display mostly photoreceptors, which in combination with the well-preserved host inner retina may be of importance in attempts at reconstructing the retina in photoreceptor degenerative disease.},
  author       = {Ghosh, Fredrik and Arnér, Karin},
  issn         = {0275-004X},
  language     = {eng},
  number       = {4},
  pages        = {478--486},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Retina},
  title        = {Transplantation of full-thickness retina in the normal porcine eye: surgical and morphologic aspects.},
  volume       = {22},
  year         = {2002},
}